Experimental Pulmonary Edema in Rabbits PDF

Experimental Pulmonary Edema in Rabbits 家兔实验性肺水肿 jiā tù shí yàn xìng fèi shuǐ zhǒng  Purpose and Principle Purpose: To learn the method of replication of experimental pulmonary edema and to understand the manifestations and mechanisms of edema.  Co...

Experimental Pulmonary Edema in Rabbits 家兔实验性肺水肿 jiā tù shí yàn xìng fèi shuǐ zhǒng  Purpose and Principle Purpose: To learn the method of replication of experimental pulmonary edema and to understand the manifestations and mechanisms of edema.  Concept of edema Edema is an abnormal accumulation of fluid in the tissue space or in one or more cavities of the body.  Pathogenesis of edema Extr bloo inters a- d titial body Intra- Intra- body vessel Extra- Extra- body vessel uè)(guǎn)(nèi)(wài)(yè)(tǐ) (jiāo)(huàn)(shī)(píng)(hén ⒈ 血管内外液体交换失平衡： Imbalance of exchange between intra- and extra-vascular fluid bloo inters d titial Four factors affect this exchange : 1. Capillary hydrostatic pressure 2. Plasma colloid osmotic pressure 3. Microvascular permeability 4. lymphatic drainage obstruction Effective filtration pressure =(Capillary filtration pressure + tissue colloidal pressure)– (tissue hydrostatic pressure+ Capillary colloidal osmotic pressure) nèi)(wài)(yè)(tǐ)(jiāo)(huàn)(shī)(píng)(h ⒉ 体内外液体交换失平衡： Imbalance of exchange between intra- and extra-body fluid Extr (nà)(shuǐ)(zhū)(liú) a- 钠、水潴留 body sodium and water are Int Retained by ra- reabsorptio n  Pulmonary Edema accumulation of fluid in the tissue space of lung  Methods and Procedures 1, normal saline solution massively and rapidly into marginal ear vein Imbalance of exchange between intra- and extra-body 2, adrenaline fluid solution elevated blood pressure Imbalance of exchange between intra- 1 Rabbit weight. Scales 2 anesthesia ： Inject 30% urethane from marginal ear vein slowly, 3.3ml/kg, then fix it on operating table. （ observe Corneal reflection and Muscle tension ） 3 Observe normal breath. Times/min 4 Inject normal saline solution: 50ml-100ml. rapidly 5 Adrenaline solution: 0.1%adrenaline 2ml + normal solution 18 ml. 6 Observe breath, lips colour, moist rales （ used by stethoscope ） , foamy fluid （ from mouth and nose ） 7 After the death, get the lungs out of the body. (Ligate trachea before cut the lung)  Weight of Lung lungs Weight (g) of Coefficient= Normal: 4-5 body (kg) Size Weight Colour Edge  Questions 1. the mechanisms of the pulmonary edema. 2. the reason of pink foamy fluid out of the mouth and in lungs.