Life Sciences Past Paper PDF - Paper 2 - 25/2023

## LIFE SCIENCES - Paper 2 **Roll No.** (Write Roll Number from left side exactly as in the Admit Card) **25/2023** **Signature of Invigilator** **Question Booklet Series** X **PAPER-II** **Question Booklet No.** **Subject Code:** 14 **LIFE SCIENCES** **Time:** 2 Hours **Maximum Marks:...

## LIFE SCIENCES - Paper 2 **Roll No.** (Write Roll Number from left side exactly as in the Admit Card) **25/2023** **Signature of Invigilator** **Question Booklet Series** X **PAPER-II** **Question Booklet No.** **Subject Code:** 14 **LIFE SCIENCES** **Time:** 2 Hours **Maximum Marks:** 200 **Instructions for the Candidates** 1. Write your Roll Number in the space provided on the top of this page as well as on the OMR Sheet provided. 2. At the commencement of the examination, the question booklet will be given to you. In the first 5 minutes, you are requested to open the booklet and verify it: * To have access to the Question Booklet, tear off the paper seal on the edge of this cover page. * Faulty booklet, if detected, should be got replaced immediately by a correct booklet from the invigilator within the period of 5 (five) minutes. Afterwards, neither the Question Booklet will be replaced nor any extra time will be given. * Verify whether the Question Booklet No. is identical with OMR Answer Sheet No.; if not, the full set is to be replaced. * After this verification is over, the Question Booklet Series and Question Booklet Number should be entered on the OMR Sheet. 3. This paper consists of One Hundred (100) multiple-choice type questions. All the questions are compulsory. Each question carries two marks. 4. Each Question has four alternative responses marked: A B C D. You have to darken the circle as indicated below on the correct response against each question. Example: A B C D where (C) is the correct response. 5. Your responses to the questions are to be indicated correctly in the OMR Sheet. If you mark your response at any place other than in the circle in the OMR Sheet, it will not be evaluated. 6. Rough work is to be done at the end of this booklet. 7. If you write your Name, Phone Number or put any mark on any part of the OMR Sheet, except in the space allotted for the relevant entries, which may disclose your identity, or use abusive language or employ any other unfair means, such as change of response by scratching or using white fluid, you will render yourself liable to disqualification. 8. Do not tamper or fold the OMR Sheet in any way. If you do so, your OMR Sheet will not be evaluated. 9. You have to return the Original OMR Sheet to the invigilator at the end of the examination compulsorily and must not carry it with you outside the Examination Hall. You are, however, allowed to carry question booklet and duplicate copy of OMR Sheet after completion of examination. 10. Use only Black Ball point pen. 11. Use of any calculator, mobile phone, electronic devices/gadgets etc. is strictly prohibited. 12. There is no negative marks for incorrect answer. **[Please Turn Over]** ## Life Sciences - Paper II **X-3** **14-II** **1. Which one of the following has a biradially symmetrical body?** * (A) Planaria * (B) Fasciola * (C) Brachionus * (D) Metridium **2. The membrane phospholipids form bilayer when water is available on all sides, due to** * (A) its amphipathic nature. * (B) presence of unsaturated fatty acids. * (C) presence of unsaturated as well as saturated fatty acids in its tail. * (D) asymmetric distribution of phospholipids and also due to presence of cholesterol. **3. Which one of the following is not a step in Southern Blot technique?** * (A) Separation of the DNA fragments on a gel. * (B) Ligation of the DNA to a vector. * (C) Transfer of the DNA fragments to a nitrocellulose membrane. * (D) Hybridization of the DNA with a labelled probe. **4. The population density of an insect species increases from 50 to 60 in one month. If the birth rate for the period is 0.4, then the death rate is** * (A) 0.2 * (B) 0.16 * (C) 0.14 * (D) 0.4 **5. A major goal of ‘Golden Rice Project' is to supply consumers in rice based societies with recommended daily intake of** * (A) Iron * (B) Vitamin C * (C) Vitamin A * (D) Vitamin D **6. For induction of somatic embryogenesis in higher plants, the cultured friable callus tissue needs to be exposed to** * (A) high concentration of auxin for short duration. * (B) high concentration of ABA for short duration. * (C) high concentration of cytokinin for long duration. * (D) low concentration of auxin for long duration. **7. Glucose-6-phosphate is a substrate or a product in all of the following pathways except** * (A) Glycolysis * (B) Gluconeogenesis * (C) β-oxidation * (D) Glycogenesis **8. Which of the following statements is correct?** * (i) Numerical taxonomy aims at determining phenetic relationships between organisms or taxa. * (ii) APG system of classification is established by taking the information from recent molecular studies. * (iii) Phylogenetic classification is constructed in such a way that all the descendants of a common ancestor should be placed in the same group, i.e., group should be monophyletic. * (A) Only (i) * (B) Only (ii) * (C) (ii) and (iii) * (D) (i), (ii) and (iii) **[ Please Turn Over ]** **X-4** **14-II** **9. Match the non-protein coding RNA molecules present in genome with their function.** | Non-protein coding RNA | Function | |---|---| | (a) snRNA | (i) X-chromosome inactivation| | (b) snoRNA | (ii) Protein synthesis | | (c) Xist | (iii) Regulation of gene expression| | (d) mi RNA | (iv) Pre rRNA splicing| | (e) rRNA | (v) mRNA splicing| **Codes:** | | (a) | (b) | (c) | (d) | (e) | |---|---|---|---|---|---| | (A) | (ii) | (iii) | (iv) | (v) | (i) | | (B) | (iii) | (v) | (iv) | (ii) | (i)| | (C) | (v) | (iv) | (i) | (iii) | (ii)| | (D) | (iv) | (i) | (iii) | (v) | (ii)| **10. Which one of the following complexes of Electron Transport System (ETS) does not account for the pumping out of protons from the mitochondrial matrix?** * (A) Complex I * (B) Complex II * (C) Complex III * (D) Complex IV **11. Chemical that is required for maintaining the protoplasts in intact condition during protoplast culture is** * (A) DMSO * (B) Mannitol * (C) PVP * (D) Sucrose **12. Individuals with greater mass have a smaller surface area to volume ratio, which helps to conserve heat. This is known as** * (A) Allen's rule * (B) Bergmann's rule * (C) Cope's rule * (D) Gloger's rule **13. In a cell fusion experiment, a cell in S-phase was fused with cells in (a) G₁-Phase, (b) G2-phase, (c) M-phase. The fused cells were grown in medium containing tritium labelled thymidine. Maximum radiolabel will be obtained in S-phase cell fused with** * (A) G₁-phase cell * (B) G2-phase cell * (C) M-phase cell * (D) G2 and M-phase cells **14. In antibody molecules, variable regions are present in** * (A) heavy chains only. * (B) light chains only. * (C) both the heavy and light chains. * (D) single heavy chain and single light chain. **15. Which of the following is a constitutive promoter for expression of transgene in monocotyledonous plants?** * (A) 35S promoter * (B) Ubiquitine gene promoter * (C) Oleosin gene promoter * (D) Rubisco gene promoter **16. The major detoxification reactions in phase-1 involved all of the following except** * (A) Oxidation * (B) Acetylation * (C) Epoxidation * (D) Hydrolysis **17. Which of the following diseases is associated with protein misfolding?** * (A) Cystic fibrosis * (B) Transmissible spongiform encephalopathy * (C) Phenylketoneuria * (D) Gaucher disease **X-5** **14-II** **18. Which one of the following statements is not true for the retinal protein bacteriorhodopsin?** * (A) It is the major photosynthetic pigment of the bacterium *Rhodopseudomonas palustris*. * (B) It converts the energy of green light (500-600 nm) into an electrochemical proton gradient. * (C) It functions as a light driven proton pump, transporting protons out of the cell. * (D) It is the only protein of the purple membrane of *Halobacterium salinarium*. **19. The pygmy hog and the hangul are the representatives of the following Indian States:** * (A) Sikkim and Assam * (B) Assam and Jammu and Kashmir * (C) Manipur and Sikkim * (D) Mizoram and Tripura **20. Which of the following statements is not true about Brown fat tissue?** * (A) It is abundant in mitochondria. * (B) It is specialized in heat generation. * (C) It is specialized for storage of fat. * (D) It is vital for survival of hybernating animals. **21. In a brackishwater shrimp farm, a farmer detected some shrimps with white spots of variable sizes prominently seen on the carapace. It could be caused by a** * (A) Baculovirus * (B) Picornavirus * (C) Reovirus * (D) Parvovirus **22. In a diploid organism segregation of the two alleles, after a recombination event between the given gene and the centromere of the chromosome, occur at** * (A) Leptotene * (B) Anaphase I * (C) Diplotene * (D) Anaphase II **23. Which one of the following serves as the source of CO2 for the Calvin cycle in C4 plants?** * (A) OAA * (B) Malic acid * (C) PEP * (D) RuBP **24. Which one of the following neurotransmitters is responsible for awakening from sleep?** * (A) Serotonin * (B) Dopamine * (C) Acetylcholine * (D) GABA **25. Acquisition of skills for playing piano is an example of** * (A) non-declarative memory * (B) declarative memory * (C) sensory habituation * (D) operant sensitization **26. Methotrexate, an anticancer drug, is an inhibitor of** * (A) DNA polymerase * (B) Dihydrofolate reductase * (C) DNA-topoisomerase * (D) Telomerase **[Please Turn Over]** **X-6** **14-II** **27. Which one of the following does not contribute to the productivity in the marine ecosystem?** * (A) Phytoplankton * (B) Diatoms and dinoflagellates * (C) Algae * (D) Molluscs and Crustaceans **28. Which one of the following statements about the nucleolus is false?** * (A) It is surrounded by a lipid bilayer membrane. * (B) It appears due to the synthetic activity of NOR region of chromosomes. * (C) It is the site of synthesis of rRNAs. * (D) Ribosome assembly begins in the nucleolus. **29. The aggregated mass of sterile cells located at the inside base of capsule of *Pellia* is called** * (A) Elaterophore * (B) Involucre * (C) Perigynum * (D) Pseudoelater **30. All of the following statements are true with respect to T-lymphocytes, except:** * (A) they arise from bone marrow and mature in the thymus. * (B) during maturation, T cells acquire a receptor called T-cell receptor on the membrane. * (C) the secretory product of T-cells include antibodies belonging to different classes. * (D) the cytotoxic T-cells are also called CD8+ cells as they have a CD8 receptor on their membrane. **31. If a *Drosophila* species has 3 pairs of metacentric chromosomes and 1 pair of telocentric chromosome, this species will have** * (A) 7 arms in polytene chromosomes. * (B) 5 arms in polytene chromosomes. * (C) 6 arms in polytene chromosomes. * (D) 9 arms in polytene chromosomes. **32. Out of following enzymes mentioned below, which one is activated by acid hydrolysis of the proenzyme form?** * (A) Pepsin * (B) Trypsin * (C) Chymotrypsin * (D) Elastage **33. Which of the following statements is not correct for the phenomenon of photoblastism?** * (A) The phenomenon of photoblastism was discovered in Lettuce seeds. * (B) Germination of photoblastic lettuce seeds is regulated by the pigment phytochrome. * (C) Phytochrome regulated lettuce seed germination is thought to be mediated by auxin. * (D) Red light irradiation induces the germination of lettuce seeds. **34. A phytohormone which is produced by plants under water deficient condition and plays a key role in the tolerance response of plants to drought is** * (A) Ethylene * (B) ABA * (C) GA3 * (D) Kinetin **X-7** **14-II** **35. Which one of the following learning processes belongs to “Associative Learning" type?** * (A) Habituation * (B) Sensitization * (C) Classical conditioning * (D) Post-synaptic facilitation **36. The restriction enzyme EcoRI recognizes and cleaves at 5' GAATTC 3' What would be the approximate number of DNA fragments produced by cleaving E. coli genome (size ~ 4.60 × 10º bp) with EcoRI?** 3' CTTAAG 5'' * (A) 546 * (B) 723 * (C) 1123 * (D) 976 **37. If you wish to study the region of binding of a transcription factor to the promoter DNA, which one of the following techniques will be most appropriate?** * (A) Chromosome walking * (B) DNA footprinting * (C) Microarray * (D) Immunoprecipitation **38. Which one of the following statements about Analysis of Variance (ANOVA) is not correct?** * (A) It is commonly used for dividing the variance into components. * (B) It is used for determining whether the differences from the mean are significant, i.e., larger than expected by chance. * (C) The analysis involves partitioning the variance and comparing the role of various factors (environmental, genetical etc.). * (D) It is calculated by adding the series of values and dividing the total by the number of values. **39. If a sample of DNA is found to have the base composition (mole ratios) as follow: Adenine-40; Thymine-22; Guanine-21 and Cytosine -17, then which one of the following conclusions will be most appropriate?** * (A) The given DNA is a single stranded molecule * (B) It is a double stranded molecule * (C) It has high melting point * (D) It is a retroviral genome **40. In red algae and in some charophycean green algae, the elongated apical portion of the female gamete (carpogonium or oogonium) that is receptive to male gamete is called** * (A) Trichogyne * (B) Trichome * (C) Trichocysts * (D) Tube cell **41. Which one of the following statements is not correct?** * (A) Hybridization between different species usually results in sterile offsprings, due to failure of pairing at meiosis. * (B) Cleistogamy is an outbreeding device. * (C) Sporophytic self-incompatibility results from genetic composition of tissues such as style and stigma which prevent self fertilization. * (D) Continuous selfing results reduced variation in populations, and accumulation of deleterious alleles. **42. What would be the frequency of occurence of Restriction site for Pst I (3'GACGTC ( 3) 5' in a DNA from thermophile with G + C content of 80% in its genome?** * (A) 3906 * (B) 3406 * (C) 6250 * (D) 3000 **[Please Turn Over]** **X-8** **14-II** **43. "Agent orange" used as leaf defoliator by US army during Vietnam war was** * (A) 2, 4-D and NAA * (B) 2, 4-D and 2, 4, 5T * (C) Ethylene * (D) Kinetin **44. Which one of the following brain centers in human is called the trouble shooter in visual function?** * (A) Lateral Geniculate Nucleus * (B) Amygdala * (C) Hippocampus * (D) Superior Colliculus **45. White tailed deers are most active during the twilight hours of dusk and dawn. Which one of the circadian rhythms describes them?** * (A) Diurnal * (B) Duskurnal * (C) Crepuscular * (D) Nocturnal **47. The following are some of the statements given for dosage compensation:** * (i) In XX individuals inactivation of one of the X-chromosome occurs at early stages of development, not at 1 cell stage * (ii) * (iii) * (iv) * (v) **Which one of the options given below has all correct statements for dosage compensation in mammals?** * (A) (i), (ii) and (iv) * (B) (iii) and (iv) * (C) (i), (ii), (iv) and (v) * (D) (i), (ii) and (v) **46. Which one of the following cutaneous receptors is not activated by steady pressure stimulus?** * (A) Krause's bulbs * (B) Markel's discs * (C) Ruffini's endings * (D) Meissner's corpuscle **48. In human, it is usually predicted that half of the babies born will be male and half will be female. Which one of the following is the most appropriate explanation?** * (A) Because of the random segregation of X-chromosomes during female meiosis. * (B) Because of the random segregation of X and Y chromosomes during meiosis. * (C) Because all eggs contain X chromosome. * (D) Because on an average, one half of all eggs produce females **X-9** **14-II** **49. Membrane potential in intact mitochondria will favour finding of which one of the following compounds after passage through the inner mitochondrial membrane?** * (A) An ionophore * (B) An electro-neutral dye * (C) A cationic dye * (D) An anionic dye **50. A series of five cell lines were created by human-mouse somatic-cell hybridizations. Each cell line was examined for the presence of human chromosomes and for the production of an enzyme. The following results were obtained:** | Cell lines | Gene product present | Human chromosomes present | |---|---|---| | a | + | 1 2 3 4 5 6 7 8 9 | | b | + | 1 2 3 4 5 6 7 8 | | c | + | 1 2 3 4 5 6 7 | | d | + | 1 2 3 4 5 6 | | e | + | 1 2 3 4 5 | **On the basis of the above results, the gene is mapped to chromosome number** * (A) 4 * (B) 5 * (C) 6 * (D) 2 **51. At which of the following sites, the partial pressure of CO2 is highest?** * (A) Alveolar gas * (B) Systemic arterial blood * (C) Exhaled gas * (D) Systemic venous blood **52. Which amino acid in a polypeptide undergoes N-linked glycosylation in the lumen of the rough endoplasmic reticulum?** * (A) Serine * (B) Threonine * (C) Asparagine * (D) Glutamine **53. Which one of the following marker systems reveals higher polymorphism?** * (A) RAPD * (B) AFLP * (C) ISSR * (D) SNP **54. *Drosophila melanogaster* and *Drosophila simulans* are morphologically similar but reproductively isolated species. They are known as** * (A) Sympatric species * (B) Allopatric species * (C) Sibling species * (D) Incipient species **55. Ex-situ bioremediation techniques involve excavation and subsequent transportation of pollutants to another site for treatment. Which of the following techniques is not a part of this process?** * (A) Bioreactor * (B) Biopile * (C) Windrow * (D) Bioventing **56. There are two statements, one is Assertion (A) and another is Reason (R).** **Assertion (A):** The carbon dioxide content of the atmosphere has been rising in past few decades due to burning of fossil fuels. **Reason (R):** On an average there is an increase in temperature of 0.4 to 0.5°C per decade. **Select the correct answer from the options given below:** * (A) Both (A) and (R) are correct and (R) is the correct result of (A). * (B) Both (A) and (R) are correct and (R) is not the correct result of (A). * (C) (A) is correct but (R) is incorrect. * (D) (A) is incorrect but (R) is correct. **[ Please Turn Over ]** **X-10** **14-II** **57. Which one of the following characters of *Anthoceros* is shared with that of green algae?** * (A) Stoma-like slime pores * (B) Intercalary meristem at the base of sporophyte * (C) Amphithecial origin of archesporium * (D) Each cell with single chloroplast having pyrenoids **58. In *Escherichia coli* the mismatch repair system repairs the mis-incorporated base which is not removed by the proof reading activity of DNA polymerase. While doing so the repair system has to decide which DNA strand is newly synthesized and which DNA strand is parental. Mismatch repair system does it by recognizing** * (A) GATC sequence nearby. * (B) specific repetitious sequence. * (C) hemimethylated GATC sequence nearby. * (D) specific pallindromic sequence nearby **59. The deposition of pollen on the stigma of another flower of the same plant is called** * (A) Homogamy * (B) Dichogamy * (C) Geitonogamy * (D) Xenogamy **60. Which one of the following options has characteristics of a non-poisonous snake?** * (A) Presence of cylindrical tail, broad transverse belly scales and 4th infra-labial scale largest. * (B) Cylindrical tail, broad transverse scales and 3rd supra-labial touches eye and nose. * (C) Presence of cylindrical tail and small belly scales. * (D) Cylindrical tail, broad transverse belly scales and a loreal pit between eye and nostril. **61. Juvenile hormone (JH) regulates development and reproduction in arthropods. Chemically it is a** * (A) derivative of amino acid. * (B) terpenoid. * (C) protein. * (D) flavonoid. **62. Mutable Collagenous Tissue (MCT) is the characteristic of the phylum:** * (A) Annelida * (B) Arthropoda * (C) Mollusca * (D) Echinodermata **63. Which of the following techniques would be the most suitable to identify a micro-deletion?** * (A) G-banding * (B) R-banding * (C) Fluorescence in situ hybridization * (D) Inversion analysis **64. Fangs are specialized teeth used for venom deliverance in animals. In snakes, solenoglyphous fangs can be seen in** * (A) Vipers * (B) Kraits * (C) Cobra * (D) Hog-nose snake **65. Baffles are metal strips attached radially to fermenter wall. The major function of the baffles is to** * (A) introduce sterile air in the medium of the fermenter tank. * (B) break the vortex formed during the agitation process. * (C) introduce the anti-foaming agent in the fermentation medium. * (D) filter the air that is coming inside the fermenter and to prevent contamination **X-11** **14-II** **66. Which of the phytohormones is primarily involved in stomatal closure under drought condition?** * (A) Ethylene * (B) ABA * (C) IAA * (D) GA3 **67. Which moiety of haemoglobin molecule binds to 2, 3, bisphosphoglycerate at pH 7.0?** * (A) Amino terminal * (B) Carboxy terminal * (C) Sulfhydryl group * (D) Alcoholic group **68. The Gram-negative, motile bacterial species *Pseudomonas carboxydovorans* is an/a** * (A) aerobic carbon monoxide oxidizing bacteria. * (B) ammonia oxidizing bacteria. * (C) sulfur oxidizing bacteria. * (D) carbon dioxide reducing bacteria. **69. In an experiment with *Xenopus* blastula, some of the animal cap cells that are supposed to be ectodermal in fate, are labelled with fluorescent dye and placed at the floor of the blastocoel. The labelled cells will be fated as** * (A) Endodermal * (B) Ectodermal * (C) Mesodermal * (D) Apoptotic **70. A blueprint for action plan for protection of The Earth, the “Agenda 21” envisages** * (A) scientific understanding of the phenomena of climate change, biodiversity and conservation. * (B) action plan for 21st century in all areas of environment and economic growth in sustainable way. * (C) global consensus on comprehensive management and conservation of forests. * (D) 21st meeting of the conference of parties (COP) to the United Nations Framework Convention on climate change (COP21). **71. Lactic acid component of human sweat attracts the mosquito, *Aedes aegypti* to have a blood meal. This chemical signal emitted by human is an example of** * (A) Pheromone * (B) Kairomone * (C) Synomone * (D) Allomone **72. The following statements are given for the genes/gene products that are involved in giving molecular address to the body parts:** * (i) Contains 60 aa homeo domain that fits mainly in the major groove of the downstream genes. * (ii) Act as transcription factors. * (iii) Have four paralogous groups located in different chromosomes. * (iv) * (v) **Which combination of above statements is the best appropriate for *Drosophila* homeotic genes/gene products?** * (A) (i), (ii), (iii) and (iv) * (B) (i) only * (C) (i), (ii) and (iv) * (D) (i) and (iv) **[ Please Turn Over ]** **X-12** **14-II** **73. Cutting the posterior root of a spinal nerve would** * (A) impair motor control of skeletal muscle. * (B) interfere with the flow of sensory impulses. * (C) interfere with the ability of brain to transmit impulses. * (D) interfere with the circulation of CSF. **74. The fish that lives in riverine water and becoming reproductively mature migrates to the sea to breed there is known as** * (A) Euryhaline fish * (B) Stenohaline fish * (C) Amphihaline potamotocous fish * (D) Amphihaline thalassotocous fish ** 75. Two large plasmids of 80 kb and 100 kb have been isolated from *Rhizobium* sp. Which of the following techniques should be most suitable to resolve these two plasmids?** * (A) PAGE * (B) Pulse field gel electrophoresis * (C) Agarose gel electrophoresis * (D) Capillary gel electrophoresis **76. The CLAVATA3 (CLV3) - WUSCMEL (WUS) signaling pathway has evolved as the central regulatory pathway that** * (A) coordinates stem cell proliferation with differentiation in shoot apical meristem. * (B) maintains the adaxial identity in developing leaf primordia. * (C) stimulates development of nitrogen fixing root nodules. * (D) alters the leaf size and shape during development. **77. A 34 years old man visits a physician with high fever, chill, severe headache and confusion. He has recently returned from Africa after completing an official assignment. A peripheral blood smear reveals multiple ring structures and crescent-shaped gametes. The most likely cause of his complaint is** * (A) *Plasmodium falciparum* * (B) *Plasmodium malariae* * (C) *Plasmodium ovale* * (D) *Plasmodium vivax* **78. 'Molten Globule State' is associated with** * (A) Nucleotide modification * (B) Lipid transport * (C) Carbohydrate synthesis * (D) Protein folding **79. Which one is a wrong match between the helminth and the disease transmitting invertebrate host?** * (A) Guinea worm - snail * (B) Filarial worm - mosquito * (C) Liver fluke - snail * (D) *Schistosoma* - snail **80. After meiosis I, each of the resultant daughter cells possesses:** * (A) Twice as much DNA as gametes contain * (B) As much DNA as gametes contain * (C) Four times as much DNA as gametes contain * (D) Half the amount of DNA as gametes contain **X-13** **14-II** **81. In snail *Lymnaea peregra*, a male homozygous for dextral alleles 's⁺s⁺ ' is crossed with a female homozygous for sinistral alleles 'ss' (s⁺ is dominant while 's' is recessive). When F1 individuals of this cross were crossed among themselves, in F2 all progeny individuals had dextral phenotype.** **Which of the following options gives a correct explanation for this result?** * (A) This is a typical example of cytoplasmic inheritance as the phenotype of female alone is being transmitted. * (B) Exhibiting gene interaction with epistasis. * (C) The trait is quantitative and environment plays a crucial role to aquire the dextral phenotype. * (D) Exhibiting genetic maternal effect. **82. Which of the following pairs of antibiotics interact with sterols in cell membrane to form channels through which small molecules leak from the inside of the fungal cell to the outside?** * (A) Amphotericin and Nystatin * (B) Amphotericin and Bacitracin * (C) Bacitracin and Vancomycin * (D) Nystatin and Cephalosporin **83. If the overhead concentration of ozone at a point above The Earth's surface is 450 DU, what is the thickness in millimeters of pure ozone at 1.0 atm pressure?** * (A) 450 * (B) 45 * (C) 4.5 * (D) 50 **84. Which class of chemical insecticides is characterized by a relatively high degree of environmental resistance?** * (A) Carbamates * (B) Chlorinated hydrocarbons * (C) Synthetic pyrethroids * (D) Organophosphates **85. A sample contains four proteins with following properties:** * Protein 1: MW 14000, PI = 10 * Protein 2: MW 62000, PI = 4 * Protein 3: MW 29000, PI = 8 * Protein 4: MW 9000, PI = 5 **What will be the order of emergence of these proteins when the mixture of four is chromatographed in anion exchange column at pH 7.0?** * (A) Protein 1 and 3 will not bind to the column * Protein 2 will be eluted first followed by protein 4 * (B) Protein 1 and 3 will not bind to the column * Protein 4 will be eluted first followed by protein 2 * (C) Protein 2 and 4 will not bind to the column * Protein 1 will be eluted first followed by protein 3 * (D) Protein 2 and 4 will not bind to the column * Protein 3 will be eluted first followed by protein 1 **[Please Turn Over]** **X-14** **14-II** **86. Three different Hfr donor strains are mixed with separate samples of an F- strain and the following mapping data are obtained from the studies of interrupted conjugation:** | Hfr 1 | Genes b+ d+ c+ f+ g+ | Time (min) 5 10 15 25 60 | |---|---|---| | Hfr 2 | Genes e+ f+ c d+ b+ | Time (min) 6 26 36 41 46 | | Hfr 3 | Genes c+ f+ e g+b+ | Time (min) 8 18 38 53 78 | **Which of the following options gives the correct constructed map?>** * (a) 5'→3' * (b) 3'→5' * (c) 5'→3' * (d)