Microbiology and Parasitology (Laboratory) - BIOL 014 PDF

Polytechnic University of the Philippines College of Science Department of Biology MICROBIOLOGY AND PARASITOLOGY (LABORATORY) BIOL 014 Laboratory Discussion 1: Introduction to Basic Laboratory Practices Trisha Mae M. Cantillano  Importance of Microorganisms in the Ecosyst...

Polytechnic University of the Philippines College of Science Department of Biology MICROBIOLOGY AND PARASITOLOGY (LABORATORY) BIOL 014 Laboratory Discussion 1: Introduction to Basic Laboratory Practices Trisha Mae M. Cantillano  Importance of Microorganisms in the Ecosystem Microorganisms are inherent part of any ecosystem.; They are ubiquitous and have profound effects on the functioning of the earth; Extensive research in microbiology has revealed that almost all ecosystem services are mediated by microbial metabolism including biogeochemical cycling of materials, degradation of pollutants, wastewater treatment, and production of oxygen. T.M.M. Cantillano Introduction to Basic Laboratory Practices  How should we see the microbes in the lab? T.M.M. Cantillano Introduction to Basic Microbiological Laboratory Practices  Importance of Studying Parasites Parasites are responsible for many diseases, especially in tropical and developing regions. Studying parasites is crucial for monitoring and controlling outbreaks, as parasites have zoonotic potential Understanding the lifecycle of parasites enable development of treatments, vaccines, and control measures T.M.M. Cantillano Introduction to Basic Laboratory Practices  Importance of learning basic practices in the lab Basic practices in a laboratory are essential to ensure the safety of laboratory personnel, prevent contamination of samples, and obtain accurate and reliable results. T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biohazard, Biosafety and Biorisk in Microbiology Biohazard – biological substances that pose a threat to health of other living organisms. Biosafety – set of rules or measures that are taken to ensure the safe handling of biological materials to prevent harm to humans, animals, or/and the environment Biorisks – is the likelihood of exposure to a biological hazard, toxin, or infectious agent that could cause harm (e.g.: accidental release of pathogens; loss of biological materials for malicious purposes; potential contamination). T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biohazard Management Pathogenic microorganisms such as bacteria (e.g., Escherichia coli, Salmonella), viruses (e.g., HIV, Ebola), fungi (e.g., Aspergillus), and parasites (e.g., Plasmodium). Toxins produced by biological agents, such as botulinum toxin or ricin. Specimens or cultures containing infectious agents that can cause harm to humans, animals, or the environment if not handled properly. T.M.M. Cantillano Introduction to Basic Microbiological Laboratory Practices  Biosafety Management Personal Protective Equipment (PPE) Hand washing and Hand Hygiene Work Area Cleaning Sterilization Waste Disposal Familiarization of materials, equipment and Procedure (MEP) T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biosafety Management Personal Protective Equipment (PPE) Hand washing and Hand Hygiene Work Area Cleaning Sterilization Waste Disposal Familiarization of materials, equipment and Procedure (MEP) T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biosafety Management Personal Protective Equipment (PPE) Hand washing and Hand Hygiene Work Area Cleaning Sterilization Waste Disposal Familiarization of materials, equipment and Procedure (MEP) T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biosafety Management Personal Protective Equipment (PPE) Hand washing and Hand Hygiene Work Area Cleaning Sterilization Waste Disposal Familiarization of materials, equipment and Procedure (MEP) T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biosafety Management Personal Protective Equipment (PPE) Hand washing and Hand Hygiene Work Area Cleaning Sterilization Waste Disposal Familiarization of materials, equipment and Procedure (MEP) T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biosafety Management T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biorisks Management and Biosafety levels Primary Containment Barriers – includes the physical barriers that you use to protect yourself Secondary Barriers – structural aspects of the laboratory that make the working environment safer T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biorisks Management and Biosafety levels Note that: Risk groups DO NOT always equate with Biosafety Levels, and Biosafety Levels DO NOT always equate with the type of Biosafety Cabinet T.M.M. Cantillano Introduction to Basic Laboratory Practices  Biorisks Management and Biosafety levels You can visit: https://my.absa.org/Riskgroups T.M.M. Cantillano Introduction to Basic Laboratory Practices  Laboratory Waste Disposal Items that are contaminated with microorganisms must be autoclaved before disposal or washing Needles and syringes must be thrown into the sharp's container Disposable petri dishes and other disposable plastic items used in culturing microorganisms must be disinfected before disposal T.M.M. Cantillano Introduction to Basic Laboratory Practices