Hemostasis and Blood Coagulation PDF

PAGE 1 UNIVERSITÄTSMEDIZIN NEUMARKT A. M. https://edu.umch.de www.umfst.ro CAMPUS HAMBURG PRESENTER: Assist. Raluca 2024 Luna V Niculescu TITLE: Hemos...

PAGE 1 UNIVERSITÄTSMEDIZIN NEUMARKT A. M. https://edu.umch.de www.umfst.ro CAMPUS HAMBURG PRESENTER: Assist. Raluca 2024 Luna V Niculescu TITLE: Hemostasis and Blood Coagulation Associate professor Adina Stoian  PAGE 2 Hemostasis Events The term hemostasis means prevention of blood loss. Whenever a vessel is damaged or ruptured, hemostasis is achieved by several mechanisms: (1) vascular constriction (2) formation of a platelet plug (3) formation of a blood clot as a result of blood coagulation (4) eventual growth of fibrous tissue into the blood clot to close the hole in the vessel permanently.  PAGE 3 Vascular Constriction Immediately after a blood vessel has been cut or ruptured, the trauma to the vessel wall causes smooth muscle in the wall to contract; this instantaneously reduces the flow of blood from the ruptured vessel. The contraction results from: (1) local myogenic spasm (2) local factors from the traumatized tissues and blood platelets (3) nervous reflexes. The nervous reflexes are initiated by pain nerve impulses or other sensory impulses that originate from the traumatized vessel or nearby tissues. Vasoconstriction contributes to hemostasis because it collapses some vessels that have an intravascular pressure below the critical closing pressure. However, even more vasoconstriction probably results from local myogenic contraction of the blood vessels initiated by the direct damage to the vascular wall.  PAGE 4 Vascular Constriction Vessel constriction is also promoted by chemical products of platelet plug formation and of coagulation. For example, activated platelets release the vasoconstrictors thromboxane A 2 and serotonin (5-HT), vasoconstrictor substances Moreover, thrombin, a major product of the clotting machinery, triggers the endothelium to generate endothelin 1 (ET-1), the most powerful physiological vasoconstrictor. The more severely a vessel is traumatized, the greater the degree of vascular spasm. The spasm can last for many minutes or even hours, during which time the processes of platelet plugging and blood coagulation can take place.  PAGE 5 Formation of the Platelet Plug If the cut in the blood vessel is very small—indeed, many very small vascular holes develop throughout the body each day—the cut is often sealed by a platelet plug rather than by a blood clot. To understand this process, it is important that we first discuss the nature of platelets themselves. Plug formation is a process that includes adhesion, activation, and aggregation. Physical and Chemical Characteristics of Platelets Platelets (also called thrombocytes ) are minute discs 1 to 4 micrometers in diameter. They are formed in the bone marrow from megakaryocytes, which are extremely large hematopoietic cells in the marrow; the megakaryocytes fragment into the minute platelets either in the bone marrow or soon after entering the blood, especially as they squeeze through capillaries. The normal concentration of platelets in the blood is between 150,000 and 300,000 per microliter.  PAGE 6 Blood and hemostasis Seifter, Julian L., MD, Integrated Physiology and Pathophysiology, 7, 100-117 The process of hematopoiesis. Pluripotent stem cells in the bone marrow divide by mitosis and give rise to progeny that commit to one of four basic developmental pathways. The erythroid pathway results in red blood cells. The phagocytic pathway pr... Copyright © 2022 Copyright © 2022 by Elsevier, Inc. All rights reserved.  PAGE 7 Physical and Chemical Characteristics of Platelets Platelets have many functional characteristics of whole cells, even though they do not have nuclei and cannot reproduce. In their cytoplasm are (1) actin and myosin molecules , which are contractile proteins similar to those found in muscle cells, and still another contractile protein, thrombosthenin, that can cause the platelets to contract; (2) residuals of both the endoplasmic reticulum and the Golgi apparatus that synthesize various enzymes and especially store large quantities of calcium ions;  PAGE 8 Physical and Chemical Characteristics of Platelets (3) mitochondria and enzyme systems that are capable of forming adenosine triphosphate (ATP) and adenosine diphosphate (ADP); (4) enzyme systems that synthesize prostaglandins, which are local hormones that cause many vascular and other local tissue reactions; (5) an important protein called fibrin-stabilizing factor, which we discuss later in relation to blood coagulation; and (6) a growth factor that causes vascular endothelial cells, vascular smooth muscle cells, and fibroblasts to multiply and grow, thus causing cellular growth that eventually helps repair damaged vascular walls.  PAGE 9 Physical and Chemical Characteristics of Platelets Thus, the platelet is an active structure. It has a half-life in the blood of 8 to 12 days, so over several weeks its functional processes run out; It is then eliminated from the circulation mainly by the tissue macrophage system. More than one half of the platelets are removed by macrophages in the spleen, where the blood passes through a latticework of tight trabeculae.  PAGE 10 Mechanism of the Platelet Plug Platelet repair of vascular openings is based on several important functions of the platelet. When platelets come in contact with a damaged vascular surface, especially with collagen fibers in the vascular wall, the platelets rapidly change their own characteristics drastically. They begin to swell; they assume irregular forms with numerous irradiating pseudopods protruding from their surfaces; their contractile proteins contract forcefully and cause the release of granules that contain multiple active factors; they become sticky so that they adhere to collagen in the tissues and to a protein called von Willebrand factor that leaks into the traumatized tissue from the plasma; they secrete large quantities of ADP; and their enzymes form thromboxane A2.  PAGE 11 Haemostasis Redhouse White, Gus, BSc (Hons), Crash Course Haematology and Immunology, 6, 67-87 Transient tethering. Circulating von Willebrand factor (vWF) binds exposed subendothelial collagen and is immobilized in an endothelial defect. Platelet GPIb receptors then bind the immobilized vWF. This allows a monolayer of platelets to form ove... Copyright © 2019 © 2019, Elsevier Limited. All rights reserved.  PAGE 12 Mechanism of the Platelet Plug The ADP and thromboxane in turn act on nearby platelets to activate them as well, and the stickiness of these additional platelets causes them to adhere to the original activated platelets. Therefore, at the site of a puncture in a blood vessel wall, the damaged vascular wall activates successively increasing numbers of platelets that attract more and more additional platelets, thus forming a platelet plug. This plug is loose at first, but it is usually successful in blocking blood loss if the vascular opening is small. Then, during the subsequent process of blood coagulation, fibrin threads form. These threads attach tightly to the platelets, thus constructing an unyielding plug.  PAGE 13 Mechanism of the Platelet Plug - Adhesion Platelets do not adhere to themselves, to other blood cells, or to endothelial membranes. One preventive factor may be the negative surface charge on both platelets and endothelial cells. In the case of endothelial cells, the negative surface charge reflects the presence of proteoglycans, mainly heparan sulfate. Platelet adhesion occurs in response to an increase in the shearing force at the surface of platelets or endothelial cells and in response to vessel injury or humoral signals. Platelet adhesion—the binding of platelets to themselves or to other components—is mediated by platelet receptors, which are glycoproteins in the platelet membrane.  PAGE 14 Mechanism of the Platelet Plug - Adhesion They are usually heterodimers linked by disulfide bonds. One ligand naturally present in the blood plasma is von Willebrand factor (vWF), a glycoprotein made by endothelial cells and megakaryocytes. vWF is found in the endothelial cells and in α granules of platelets. High shear, certain cytokines, and hypoxia all trigger the release of vWF from endothelial cells. vWF binds to the platelet receptor known as glycoprotein Ib/Ia (Gp Ib/Ia) A breach of the endothelium exposes platelet receptors to ligands that are components of the subendothelial matrix.  PAGE 15 Mechanism of the Platelet Plug - Activation The binding of these ligands—or of certain other agents (e.g., thrombin)—triggers a conformational change in the platelet receptors that initiates an intracellular signaling cascade, which leads to an exocytotic event known as the release reaction or platelet activation. The signal-transduction cascade involves the activation of phospholipase C and an influx of Ca 2+. Activated platelets exocytose the contents of their dense storage granules, which include ATP, ADP, serotonin, and Ca 2+.  PAGE 16 Mechanism of the Platelet Plug - Activation Activated platelets also exocytose the contents of their α granules, which contain several proteins, including a host of growth factors and three hemostatic factors: vWF and two clotting factors that we will discuss below, clotting factor V and fibrinogen. Activated platelets also use cyclooxygenase to initiate the breakdown of arachidonic acid to thromboxane A2, which they release. Platelet activation is also associated with marked cytoskeletal and morphological changes as the platelet extends first a broad lamellipodium and then many finger-like filopodia.  PAGE 17 Mechanism of the Platelet Plug - Aggregation Signaling molecules released by activated platelets amplify the platelet activation response. ADP, serotonin, and thromboxane A2 all activate additional platelets, and this recruitment promotes platelet aggregation. Aspirin, an inhibitor of cyclooxygenase, inhibits clotting by reducing the release of thromboxane A2. Another antiplatelet agent—clopidogrel—acts by inhibiting the receptors on the platelet surface. As noted before, vWF released by activated platelets binds to the platelet receptor Gp Ib/Ia, thereby activating even more platelets and forming molecular bridges between platelets. Platelet activation also induces a conformational change in Gp IIb/IIIa, another platelet receptor, endowing it with the capacity to bind fibrinogen. Thus, as a result of the conformational change in Gp IIb/IIIa, the fibrinogen that is always present in blood forms bridges between platelets and participates in the formation of a platelet plug.  PAGE 18 Hemostasis and Blood Coagulation Hall, John E., PhD, Guyton and Hall Textbook of Medical Physiology, Chapter 37, 477-488 Formation of a platelet plug in a severed blood vessel. Endothelial injury and exposure of the vascular extracellular matrix facilitates platelet adhesions and activation, which changes their shape and causes release of adenosine diphosphate (ADP)... Copyright © 2021 Copyright © 2021 by Elsevier, Inc. All rights reserved.  PAGE 19 Importance of the Platelet Mechanism for Closing Vascular Holes The platelet-plugging mechanism is extremely important for closing minute ruptures in very small blood vessels that occur many thousands of times daily. Indeed, multiple small holes through the endothelial cells themselves are often closed by platelets actually fusing with the endothelial cells to form additional endothelial cell membrane. Literally thousands of small hemorrhagic areas develop each day under the skin and throughout the internal tissues of a person who has few blood platelets. This phenomenon does not occur in persons with normal numbers of platelets.  PAGE 20 A controlled cascade of proteolysis creates a blood clot A blood clot is a semisolid mass composed of both platelets and fibrin and—entrapped in the mesh of fibrin—erythrocytes, leukocytes, and serum. A thrombus is also a blood clot, but the term is usually reserved for an intravascular clot. Thus, the blood clot formed at the site of a skin wound would usually not be called a thrombus. The relative composition of thrombi varies with the site of thrombosis. A higher proportion of platelets is present in clots of the arterial circulation, whereas a higher proportion of fibrin is present in clots of the venous circulation. Platelet plug formation and blood clotting are related but distinct events that may occur in parallel or in the absence of one other.  PAGE 21 A controlled cascade of proteolysis creates a blood clot Activated platelets can release small amounts of some of the factors (e.g., Ca2+) that play a role in blood clotting. Conversely, as we have already noted, some clotting factors (e.g., thrombin and fibrinogen) play a role in platelet plug formation. Thus, molecular crosstalk between the machinery involved in platelet plug formation and clot formation helps coordinate hemostasis. The cardiovascular system normally maintains a precarious balance between two pathological states. On the one hand, inadequate clotting would lead to the leakage of blood from the vascular system and, ultimately, to hypovolemia. On the other hand, overactive clotting would lead to thrombosis and, ultimately, to cessation of blood flow.  PAGE 22 A controlled cascade of proteolysis creates a blood clot The cardiovascular system achieves this balance between an antithrombotic (anticoagulant) and a prothrombotic (procoagulant) state by a variety of components of the vascular wall and blood. Promoting an antithrombotic state is a normal layer of endothelial cells, which line all luminal surfaces of the vascular system. Promoting a prothrombotic state are events associated with vascular damage: (1) the failure of endothelial cells to produce the proper antithrombotic factors (2) the physical removal or injury of endothelial cells, which permits the blood to come into contact with thrombogenic factors that lie beneath the endothelium. Also promoting a prothrombotic state is the activation of platelets by any of the ligands that bind to platelet receptors.  PAGE 23 Blood Coagulation in the Ruptured Vessel The third mechanism for hemostasis is formation of the blood clot. The clot begins to develop in 15 to 20 seconds if the trauma to the vascular wall has been severe and in 1 to 2 minutes if the trauma has been minor. Activator substances from the traumatized vascular wall, from platelets, and from blood proteins adhering to the traumatized vascular wall initiate the clotting process. Within 3 to 6 minutes after rupture of a vessel, the entire opening or broken end of the vessel is filled with clot if the vessel opening is not too large. After 20 minutes to an hour, the clot retracts, which closes the vessel still further. Platelets also play an important role in this clot retraction, as discussed later.  PAGE 24 Vascular Constriction Hemostasis and Blood Coagulation Hall, John E., PhD, Guyton and Hall Textbook of Medical Physiology, Chapter 37, 483-494 Clotting process in a traumatized blood vessel. (Modified from Seegers WH: Hemostatic Agents, 1948. Courtesy Charles C. Thomas, Springfield, Ill.) Copyright © 2016 Copyright © 2016 by Elsevier, Inc. All rights reserved.  PAGE 25 Fibrous Organization or Dissolution of the Blood Clot Once a blood clot has formed, it can follow one of two courses: (1) It can become invaded by fibroblasts, which subsequently form connective tissue all through the clot, or 2) it can dissolve. The usual course for a clot that forms in a small hole of a vessel wall is invasion by fibroblasts, beginning within a few hours after the clot is formed (which is promoted at least partially by growth factor secreted by platelets). This process continues to complete organization of the clot into fibrous tissue within about 1 to 2 weeks. Conversely, when excess blood has leaked into the tissues and tissue clots have occurred where they are not needed, special substances within the clot itself usually become activated. These substances function as enzymes to dissolve the clot, as discussed later.  PAGE 26 Mechanism of Blood Coagulation More than 50 important substances that cause or affect blood coagulation have been found in the blood and in the tissues—some that promote coagulation, called procoagulants , and others that inhibit coagulation, called anticoagulants. Whether blood will coagulate depends on the balance between these two groups of substances. In the blood stream, the anticoagulants normally predominate, so the blood does not coagulate while it is circulating in the blood vessels. However, when a vessel is ruptured, procoagulants from the area of tissue damage become “activated” and override the anticoagulants, and then a clot does develop.  PAGE 27 Mechanism of Blood Coagulation Clotting takes place in three essential steps: 1. In response to rupture of the vessel or damage to the blood itself, a complex cascade of chemical reactions occurs in the blood involving more than a dozen blood coagulation factors. The net result is formation of a complex of activated substances collectively called prothrombin activator. 2. The prothrombin activator catalyzes conversion of prothrombin into thrombin. 3. The thrombin acts as an enzyme to convert fibrinogen into fibrin fibers that enmesh platelets, blood cells, and plasma to form the clot. We will first discuss the mechanism by which the blood clot itself is formed, beginning with conversion of prothrombin to thrombin, and then come back to the initiating stages in the clotting process by which prothrombin activator is formed.  PAGE 28 Conversion of Prothrombin to Thrombin First, prothrombin activator is formed as a result of rupture of a blood vessel or as a result of damage to special substances in the blood. Second, the prothrombin activator, in the presence of sufficient amounts of ionic calcium (Ca ++ ), causes conversion of prothrombin to thrombin. Third, the thrombin causes polymerization of fibrinogen molecules into fibrin fibers within another 10 to 15 seconds. Thus, the rate-limiting factor in causing blood coagulation is usually the formation of prothrombin activator and not the subsequent reactions beyond that point, because these terminal steps normally occur rapidly to form the clot. Platelets also play an important role in the conversion of prothrombin to thrombin because much of the prothrombin first attaches to prothrombin receptors on the platelets already bound to the damaged tissue.  PAGE 29 Conversion of Prothrombin to Thrombin Hemostasis and Blood Coagulation Hall, John E., PhD, Guyton and Hall Textbook of Medical Physiology, Chapter 37, 483-494 Schema for conversion of prothrombin to thrombin and polymerization of fibrinogen to form fibrin fibers. Copyright © 2016 Copyright © 2016 by Elsevier, Inc. All rights reserved.  PAGE 30 Prothrombin and Thrombin Prothrombin is a plasma protein, an α2-globulin, having a molecular weight of 68,700. It is present in normal plasma in a concentration of about 15 mg/dl. It is an unstable protein that can split easily into smaller compounds, one of which is thrombin, which has a molecular weight of 33,700, almost exactly one half that of prothrombin. Prothrombin is formed continually by the liver, and it is continually being used throughout the body for blood clotting. If the liver fails to produce prothrombin, in a day or so prothrombin concentration in the plasma falls too low to provide normal blood coagulation. Vitamin K is required by the liver for normal activation of prothrombin, as well as a few other clotting factors. Therefore, either lack of vitamin K or the presence of liver disease that prevents normal prothrombin formation can decrease the prothrombin to such a low level that a bleeding tendency results.  PAGE 31 Conversion of Fibrinogen to Fibrin—Formation of the Clot Fibrinogen Formed in the Liver Is Essential for Clot Formation Fibrinogen is a high-molecular-weight protein (molecular weight = 340,000) that occurs in the plasma in quantities of 100 to 700 mg/dl. Fibrinogen is formed in the liver, and liver disease can decrease the concentration of circulating fibrinogen, as it does the concentration of prothrombin, pointed out earlier. Because of its large molecular size, little fibrinogen normally leaks from the blood vessels into the interstitial fluids, and because fibrinogen is one of the essential factors in the coagulation process, interstitial fluids ordinarily do not coagulate. Yet, when the permeability of the capillaries becomes pathologically increased, fibrinogen does leak into the tissue fluids in sufficient quantities to allow clotting of these fluids in much the same way that plasma and whole blood can clot.  PAGE 32 Action of Thrombin on Fibrinogen to Form Fibrin Thrombin is a protein enzyme with weak proteolytic capabilities. It acts on fibrinogen to remove four low-molecular-weight peptides from each molecule of fibrinogen, forming one molecule of fibrin monomer that has the automatic capability to polymerize with other fibrin monomer molecules to form fibrin fibers. Therefore, many fibrin monomer molecules polymerize within seconds into long fibrin fibers that constitute the reticulum of the blood clot. In the early stages of polymerization, the fibrin monomer molecules are held together by weak noncovalent hydrogen bonding, and the newly forming fibers are not cross-linked with one another; therefore, the resultant clot is weak and can be broken apart with ease.  PAGE 33 Action of Thrombin on Fibrinogen to Form Fibrin However, another process occurs during the next few minutes that greatly strengthens the fibrin reticulum. This process involves a substance called fibrin-stabilizing factor that is present in small amounts in normal plasma globulins but is also released from platelets entrapped in the clot. Before fibrin-stabilizing factor can have an effect on the fibrin fibers, it must be activated. The same thrombin that causes fibrin formation also activates the fibrin-stabilizing factor. This activated substance then operates as an enzyme to cause covalent bonds between more and more of the fibrin monomer molecules, as well as multiple cross-linkages between adjacent fibrin fibers, thus adding tremendously to the three-dimensional strength of the fibrin meshwork.  PAGE 34 Blood Clot. Clot Retraction and Expression of Serum. The clot is composed of a meshwork of fibrin fibers running in all directions and entrapping blood cells, platelets, and plasma. The fibrin fibers also adhere to damaged surfaces of blood vessels; therefore, the blood clot becomes adherent to any vascular opening and thereby prevents further blood loss. Within a few minutes after a clot is formed, it begins to contract and usually expresses most of the fluid from the clot within 20 to 60 minutes. The fluid expressed is called serum because all its fibrinogen and most of the other clotting factors have been removed; in this way, serum differs from plasma. Serum cannot clot because it lacks these factors.  PAGE 35 Blood Clot. Clot Retraction and Expression of Serum. Platelets are necessary for clot retraction to occur. Therefore, failure of clot retraction is an indication that the number of platelets in the circulating blood might be low. Electron micrographs of platelets in blood clots show that they become attached to the fibrin fibers in such a way that they actually bond different fibers together. Furthermore, platelets entrapped in the clot continue to release procoagulant substances, one of the most important of which is fibrin-stabilizing factor, which causes more and more cross-linking bonds between adjacent fibrin fibers.  PAGE 36 Blood Clot. Clot Retraction and Expression of Serum. In addition, the platelets contribute directly to clot contraction by activating platelet thrombosthenin, actin, and myosin molecules, which are all contractile proteins in the platelets and cause strong contraction of the platelet spicules attached to the fibrin. This action also helps compress the fibrin meshwork into a smaller mass. The contraction is activated and accelerated by thrombin, as well as by calcium ions released from calcium stores in the mitochondria, endoplasmic reticulum, and Golgi apparatus of the platelets. As the clot retracts, the edges of the broken blood vessel are pulled together, thus contributing still further to hemostasis.  PAGE 37 Positive Feedback of Clot Formation Once a blood clot has started to develop, it normally extends within minutes into the surrounding blood— that is, the clot initiates a positive feedback to promote more clotting. One of the most important causes of this clot promotion is that the proteolytic action of thrombin allows it to act on many of the other blood-clotting factors in addition to fibrinogen. For instance, thrombin has a direct proteolytic effect on prothrombin, tending to convert this into still more thrombin, and it acts on some of the blood-clotting factors responsible for formation of prothrombin activator. Once a critical amount of thrombin is formed, a positive feedback develops that causes still more blood clotting and more and more thrombin to be formed; thus, the blood clot continues to grow until blood leakage ceases.  PAGE 38 Blood and hemostasis Seifter, Julian L., MD, Integrated Physiology and Pathophysiology, 7, 100-117 Normal hemostasis. (A) After vascular injury, neurohumoral factors induce transient vasoconstriction. (B) Platelets bind via glycoprotein Ib ( GpIb) receptors to von Willebrand factor ( vWF) on exposed extracellular matrix ( ECM) and are activated... Copyright © 2022 Copyright © 2022 by Elsevier, Inc. All rights reserved.  PAGE 39 Initiation of Coagulation: Formation of Prothrombin Activator These mechanisms are set into play by (1) trauma to the vascular wall and adjacent tissues, (2) trauma to the blood, (3) contact of the blood with damaged endothelial cells or with collagen and other tissue elements outside the blood vessel. In each instance, this leads to the formation of prothrombin activator, which then causes prothrombin conversion to thrombin and all the subsequent clotting steps. Prothrombin activator is generally considered to be formed in two ways, although, in reality, the two ways interact constantly with each other: (1) by the extrinsic pathway that begins with trauma to the vascular wall and surrounding tissues and (2) by the intrinsic pathway that begins in the blood.  PAGE 40 Initiation of Coagulation According to the classical view, two distinct sequences can precipitate coagulation: the intrinsic pathway and the extrinsic pathway. It is the intrinsic pathway that becomes activated when blood comes into contact with a negatively charged surface—in the laboratory we can mimic this process by putting blood into a glass test tube. The extrinsic pathway is activated when blood comes in contact with material from damaged cell membranes. In both cases, the precipitating event triggers a chain reaction that converts precursors into activated factors, which in turn catalyze the conversion of other precursors into other activated factors, and so on.  PAGE 41 Initiation of Coagulation Most of these “precursors” are zymogens that give rise to “activated factors” that are serine proteases. Thus, controlled proteolysis plays a central role in amplifying the clotting signals. However, the cascades do not occur in the fluid phase of the blood, where the concentration of each of these factors is low. In the case of the intrinsic pathway, the chain reaction occurs mainly at the membrane of activated platelets. In the case of the extrinsic pathway, the reactions occur mainly at a “tissue factor” that is membrane bound. Both pathways converge on a common pathway that culminates in generation of thrombin and, ultimately, “stable” fibrin. PAGE 42  PAGE 43 Initiation of Coagulation: Formation of Prothrombin Activator In both the extrinsic and the intrinsic pathways, a series of different plasma proteins called blood-clotting factors play a major role. Most of these proteins are inactive forms of proteolytic enzymes. When converted to the active forms, their enzymatic actions cause the successive, cascading reactions of the clotting process. Most of the clotting factors, which are listed in previous table are designated by Roman numerals. To indicate the activated form of the factor, a small letter “a” is added after the Roman numeral, such as Factor VIIIa to indicate the activated state of Factor VIII.  PAGE 44 Extrinsic Pathway for Initiating Clotting (Tissue factor Activation) The right branch of the figure shows the extrinsic pathway, a cascade of protease reactions initiated by factors that are outside the vascular system. Nonvascular cells constitutively express an integral membrane protein called tissue factor (tissue thromboplastin, or factor III), which is a receptor for a plasma protein called factor VII. When an injury to the endothelium allows factor VII to come into contact with tissue factor, the tissue factor nonproteolytically activates factor VII to factor VIIa. Subsequently, tissue factor, factor VIIa, and Ca 2+ form a trimolecular complex that proteolytically cleaves the proenzyme factor X to factor Xa. An interesting feature is that when factor X binds to the trimolecular complex, factor VIIa undergoes a conformational change that prevents it from dissociating from tissue factor. Regardless of whether factor Xa arises by the intrinsic or extrinsic pathway, the cascade proceeds along the common pathway.  PAGE 45 Extrinsic Pathway for Initiating Clotting (Tissue factor Activation) Blood Boulpaep, Emile L., Medical Physiology, Chapter 18, 429-446.e1 Coagulation cascade, showing only the procoagulant factors. TF, tissue factor. Copyright © 2017 Copyright © 2017 by Elsevier, Inc. All rights reserved.  PAGE 46 Extrinsic Pathway for Initiating Clotting The extrinsic pathway for initiating the formation of prothrombin activator begins with a traumatized vascular wall or traumatized extravascular tissues that come in contact with the blood. This condition leads to the following steps, as shown in figure: 1. Release of tissue factor. Traumatized tissue releases a complex of several factors called tissue factor or tissue thromboplastin. This factor is composed especially of phospholipids from the membranes of the tissue plus a lipoprotein complex that functions mainly as a proteolytic enzyme. 2. Activation of Factor X—role of Factor VII and tissue factor. The lipoprotein complex of tissue factor further complexes with blood coagulation Factor VII and, in the presence of calcium ions, acts enzymatically on Factor X to form activated Factor X (Xa).  PAGE 47 Extrinsic Pathway for Initiating Clotting Hemostasis and Blood Coagulation Hall, John E., PhD, Guyton and Hall Textbook of Medical Physiology, Chapter 37, 483-494 Extrinsic pathway for initiating blood clotting. Copyright © 2016 Copyright © 2016 by Elsevier, Inc. All rights reserved.  PAGE 48 Extrinsic Pathway for Initiating Clotting 3. Effect of Xa to form prothrombin activator—role of Factor V. The activated Factor X combines immediately with tissue phospholipids that are part of tissue factors or with additional phospholipids released from platelets, as well as with Factor V, to form the complex called prothrombin activator. Within a few seconds, in the presence of Ca ++, prothrombin is split to form thrombin, and the clotting process proceeds as already explained. At first, the Factor V in the prothrombin activator complex is inactive, but once clotting begins and thrombin begins to form, the proteolytic action of thrombin activates Factor V. This activation then becomes an additional strong accelerator of prothrombin activation. Thus, in the final prothrombin activator complex, activated Factor X is the actual protease that causes splitting of prothrombin to form thrombin; activated Factor V greatly accelerates this protease activity, and platelet phospholipids act as a vehicle that further accelerates the process. Note especially the positive feedback effect of thrombin, acting through Factor V, to accelerate the entire process once it begins.  PAGE 49 Extrinsic Pathway for Initiating Clotting Hemostasis and Blood Coagulation Hall, John E., PhD, Guyton and Hall Textbook of Medical Physiology, Chapter 37, 483-494 Extrinsic pathway for initiating blood clotting. Copyright © 2016 Copyright © 2016 by Elsevier, Inc. All rights reserved.  PAGE 50 Intrinsic Pathway for Initiating Clotting (Surface Contact Activation) The left branch of the next figure shows the intrinsic pathway, a cascade of protease reactions initiated by factors that are all present within blood. When in contact with a negatively charged surface such as glass or the membrane of an activated platelet, a plasma protein called factor XII (Hageman factor) can become factor XIIa —the suffix a indicates that this is the activated form of factor XII. A molecule called high-molecular-weight kininogen (HMWK), a product of platelets that may in fact be attached to the platelet membrane, helps anchor factor XII to the charged surface and thus serves as a cofactor. However, this HMWK-assisted conversion of factor XII to factor XIIa is limited in speed. Once a small amount of factor XIIa accumulates, this protease converts prekallikrein to kallikrein, with HMWK as an anchor. In turn, the newly produced kallikrein accelerates the conversion of factor XII to factor XIIa—an example of positive feedback.  PAGE 51 Intrinsic Pathway for Initiating Clotting (Surface Contact Activation) Blood Boulpaep, Emile L., Medical Physiology, Chapter 18, 429-446.e1 Coagulation cascade, showing only the procoagulant factors. TF, tissue factor. Copyright © 2017 Copyright © 2017 by Elsevier, Inc. All rights reserved.  PAGE 52 Intrinsic Pathway for Initiating Clotting The second mechanism for initiating formation of prothrombin activator, and therefore for initiating clotting, begins with trauma to the blood or exposure of the blood to collagen from a traumatized blood vessel wall. Then the process continues through the series of cascading reactions. 1. Blood trauma causes (1) activation of Factor XII and (2) release of platelet phospholipids. Trauma to the blood or exposure of the blood to vascular wall collagen alters two important clotting factors in the blood: Factor XII and the platelets. When Factor XII is disturbed, such as by coming into contact with collagen or with a wettable surface such as glass, it takes on a new molecular configuration that converts it into a proteolytic enzyme called “activated Factor XII.” Simultaneously, the blood trauma also damages the platelets because of adherence to either collagen or a wettable surface (or by damage in other ways), and this releases platelet phospholipids that contain the lipoprotein called platelet factor 3, which also plays a role in subsequent clotting reactions.  PAGE 53 Intrinsic Pathway for Initiating Clotting 2. Activation of Factor XI. The activated Factor XII acts enzymatically on Factor XI to activate this factor as well, which is the second step in the intrinsic pathway. This reaction also requires high-molecular-weight kininogen and is accelerated by prekallikrein. 3. Activation of Factor IX by activated Factor XI. The activated Factor XI then acts enzymatically on Factor IX to activate this factor as well. 4. Activation of Factor X—role of Factor VIII. The activated Factor IX, acting in concert with activated Factor VIII and with the platelet phospholipids and Factor III from the traumatized platelets, activates Factor X. It is clear that when either Factor VIII or platelets are in short supply, this step is deficient. Factor VIII is the factor that is missing in a person who has classic hemophilia, for which reason it is called antihemophilic factor. Platelets are the clotting factor that is lacking in the bleeding disease called thrombocytopenia.  PAGE 54 Intrinsic Pathway for Initiating Clotting 5. Action of activated Factor X to form prothrombin activator—role of Factor V. This step in the intrinsic pathway is the same as the last step in the extrinsic pathway. That is, activated Factor X combines with Factor V and platelet or tissue phospholipids to form the complex called prothrombin activator. The prothrombin activator in turn initiates within seconds the cleavage of prothrombin to form thrombin, thereby setting into motion the final clotting process, as described earlier.  PAGE 55 Intrinsic Pathway for Initiating Clotting Hemostasis and Blood Coagulation Hall, John E., PhD, Guyton and Hall Textbook of Medical Physiology, Chapter 37, 483-494 Intrinsic pathway for initiating blood clotting. Copyright © 2016 Copyright © 2016 by Elsevier, Inc. All rights reserved.  PAGE 56 Common Pathway Factor Xa from either the intrinsic or extrinsic pathway is the first protease of the common pathway (center of the figure). Reminiscent of the conversion of factor VIII to the cofactor VIIIa in the intrinsic pathway, the downstream product thrombin clips factor V to form the cofactor Va. Factor V is highly homologous to factor VIII, and in both cases the proteolytic activation clips a single protein into two peptides that remain attached to one another. Factors Xa and Va, together with Ca 2+ and phospholipids, form yet another trimolecular complex called prothrombinase. Prothrombinase acts on a plasma protein called prothrombin to form thrombin.  PAGE 57 Common Pathway Blood Boulpaep, Emile L., Medical Physiology, Chapter 18, 429-446.e1 Coagulation cascade, showing only the procoagulant factors. TF, tissue factor. Copyright © 2017 Copyright © 2017 by Elsevier, Inc. All rights reserved.  PAGE 58 Role of Calcium Ions in the Intrinsic and Extrinsic Pathways Except for the first two steps in the intrinsic pathway, calcium ions are required for promotion or acceleration of all the blood-clotting reactions. Therefore, in the absence of calcium ions, blood clotting by either pathway does not occur. However, when blood is removed from a person, it can be prevented from clotting by reducing the calcium ion concentration below the threshold level for clotting, either by deionizing the calcium by causing it to react with substances such as citrate ion or by precipitating the calcium with substances such as oxalate ion. Interaction between the Extrinsic and Intrinsic Pathways—Summary of Blood- PAGE 59 Clotting Initiation It is clear from the schemas of the intrinsic and extrinsic systems that after blood vessels rupture, clotting occurs by both pathways simultaneously. Tissue factor initiates the extrinsic pathway, whereas contact of Factor XII and platelets with collagen in the vascular wall initiates the intrinsic pathway. An especially important difference between the extrinsic and intrinsic pathways is that the extrinsic pathway can be explosive; once initiated, its speed of completion to the final clot is limited only by the amount of tissue factor released from the traumatized tissues and by the quantities of Factors X, VII, and V in the blood. With severe tissue trauma, clotting can occur in as little as 15 seconds. The intrinsic pathway is much slower to proceed, usually requiring 1 to 6 minutes to cause clotting. Intravascular Anticoagulants Prevent Blood Clotting in the Normal Vascular System PAGE 60 Endothelial Surface Factors Probably the most important factors for preventing clotting in the normal vascular system are (1) the smoothness of the endothelial cell surface, which prevents contact activation of the intrinsic clotting system; (2) a layer of glycocalyx on the endothelium (glycocalyx is a mucopolysaccharide adsorbed to the surfaces of the endothelial cells), which repels clotting factors and platelets, thereby preventing activation of clotting; and (3) a protein bound with the endothelial membrane, thrombomodulin, which binds thrombin. Not only does the binding of thrombin with thrombomodulin slow the clotting process by removing thrombin, but the thrombomodulin-thrombin complex also activates a plasma protein, protein C, that acts as an anticoagulant by inactivating activated Factors V and VIII. When the endothelial wall is damaged, its smoothness and its glycocalyx-thrombomodulin layer are lost, which activates both Factor XII and the platelets, thus setting off the intrinsic pathway of clotting. If Factor XII and platelets come in contact with the subendothelial collagen, the activation is even more powerful.  PAGE 61 Anticoagulant Factors endothelial cells also generate anticoagulant factors that interfere with the clotting cascade that generates fibrin. 1. Tissue factor pathway inhibitor (TFPI). TFPI is a plasma protein that binds to the trimolecular complex [tissue factor + factor VIIa + Ca 2+ ] in the extrinsic pathway and blocks the protease activity of factor VIIa. 2. Antithrombin III (AT III). AT III binds to and inhibits factor Xa and thrombin. The sulfated glycosaminoglycans heparan sulfate and heparin enhance the binding of AT III to factor Xa or to thrombin, thus inhibiting coagulation. Heparan sulfate is present on the external surface of most cells, including endothelial surfaces. Mast cells and basophils release heparin.  PAGE 62 Haemostasis Redhouse White, Gus, BSc (Hons), Crash Course Haematology and Immunology, 6, 67-87 Overview of haemostasis. vWF, von Willebrand factor. Copyright © 2019 © 2019, Elsevier Limited. All rights reserved.  PAGE 63 Anticoagulant Factors 3. Thrombomodulin. A glycosaminoglycan product of endothelial cells, thrombomodulin can form a complex with thrombin, thereby removing thrombin from the circulation and inhibiting coagulation. In addition, thrombomodulin also binds protein C. 4. Protein C. After protein C binds to the thrombomodulin portion of the thrombin-thrombomodulin complex, the thrombin activates protein C. Activated protein C (C a ) is a protease. Together with its cofactor protein S, activated protein C inactivates the cofactors Va and VIIIa, thus inhibiting coagulation. 5. Protein S. This is the cofactor of protein C and is thus an anticoagulant.  PAGE 64 Anticoagulant Factors Blood Boulpaep, Emile L., Medical Physiology, Chapter 18, 429-446.e1 Abbreviated version of the coagulation cascade, showing the anticoagulant factors. The anticoagulant pathways are indicated in red. TF, tissue factor. Copyright © 2017 Copyright © 2017 by Elsevier, Inc. All rights reserved.  PAGE 65 Antithrombin Action of Fibrin and Antithrombin III Among the most important anticoagulants in the blood are those that remove thrombin from the blood. The most powerful of these are (1) the fibrin fibers that are formed during the process of clotting and (2) an α-globulin called antithrombin III or antithrombin-heparin cofactor. While a clot is forming, about 85 to 90 percent of the thrombin formed from the prothrombin becomes adsorbed to the fibrin fibers as they develop. This adsorption helps prevent the spread of thrombin into the remaining blood and, therefore, prevents excessive spread of the clot. The thrombin that does not adsorb to the fibrin fibers soon combines with antithrombin III, which further blocks the effect of the thrombin on the fibrinogen and then also inactivates the thrombin itself during the next 12 to 20 minutes.  PAGE 66 Heparin Heparin is another powerful anticoagulant, but because its concentration in the blood is normally low, it has significant anticoagulant effects only under special physiological conditions. However, heparin is used widely as a pharmacological agent in medical practice in much higher concentrations to prevent intravascular clotting. By itself, it has little or no anticoagulant properties, but when it combines with antithrombin III, the effectiveness of antithrombin III for removing thrombin increases by a hundredfold to a thousandfold, and thus it acts as an anticoagulant. Therefore, in the presence of excess heparin, removal of free thrombin from the circulating blood by antithrombin III is almost instantaneous. The complex of heparin and antithrombin III removes several other activated coagulation factors in addition to thrombin, further enhancing the effectiveness of anticoagulation. The others include activated Factors XII, XI, X, and IX.  PAGE 67 Heparin Heparin is produced by many different cells of the body, but the largest quantities are formed by the basophilic mast cells located in the pericapillary connective tissue throughout the body. These cells continually secrete small quantities of heparin that diffuse into the circulatory system. The basophil cells of the blood, which are functionally almost identical to the mast cells, release small quantities of heparin into the plasma. Mast cells are abundant in tissue surrounding the capillaries of the lungs and, to a lesser extent, capillaries of the liver. It is easy to understand why large quantities of heparin might be needed in these areas because the capillaries of the lungs and liver receive many embolic clots formed in slowly flowing venous blood; suffi- cient formation of heparin prevents further growth of the clots.  PAGE 68 Plasmin Causes Lysis of Blood Clots The plasma proteins contain an euglobulin called plasminogen (or profibrinolysin) that, when activated, becomes a substance called plasmin (or fibrinolysin). Plasmin is a proteolytic enzyme that resembles trypsin, the most important proteolytic digestive enzyme of pancreatic secretion. Plasmin digests fibrin fibers and some other protein coagulants such as fibrinogen, Factor V, Factor VIII, prothrombin, and Factor XII. Therefore, whenever plasmin is formed, it can cause lysis of a clot by destroying many of the clotting factors, thereby sometimes even causing hypocoagulability of the blood.  PAGE 69 Activation of Plasminogen to Form Plasmin, Then Lysis of Clots When a clot is formed, a large amount of plasminogen is trapped in the clot along with other plasma proteins. This will not become plasmin or cause lysis of the clot until it is activated. The injured tissues and vascular endothelium very slowly release a powerful activator called tissue plasminogen activator (t-PA); a few days later, after the clot has stopped the bleeding, t-PA eventually converts plasminogen to plasmin, which in turn removes the remaining unnecessary blood clot. In fact, many small blood vessels in which blood flow has been blocked by clots are reopened by this mechanism. Thus, an especially important function of the plasmin system is to remove minute clots from millions of tiny peripheral vessels that eventually would become occluded were there no way to clear them.  PAGE 70 Fibrinolysis breaks up clots Cross-linked stable fibrin traps RBCs and WBCs as well as platelets in a freshly formed thrombus. Through the interaction of actin and myosin in the platelets, the clot shrinks to a plug and thereby expels serum. After plug formation, fibrinolysis—the breakdown of stable fibrin—breaks up the clot in a more general process known as thrombolysis. As shown in next figure, the process of fibrinolysis begins with the conversion of plasminogen to plasmin, catalyzed by one of two activators: tissue-type plasminogen activator or urokinase-type plasminogen activator.  PAGE 71 Fibrinolysis breaks up clots Blood Boulpaep, Emile L., Medical Physiology, Chapter 18, 429-446.e1 Fibrinolytic cascade. Copyright © 2017 Copyright © 2017 by Elsevier, Inc. All rights reserved.  PAGE 72 Conditions That Cause Excessive Bleeding in Humans Excessive bleeding can result from a deficiency of any one of the many blood-clotting factors. Three particular types of bleeding tendencies that have been studied to the greatest extent are discussed here: bleeding caused by (1) vitamin K deficiency, (2) hemophilia, and (3) thrombocytopenia (platelet deficiency). Decreased Prothrombin, Factor VII, Factor IX, and Factor X Caused by Vitamin K PAGE 73 Deficiency With few exceptions, almost all the blood-clotting factors are formed by the liver. Therefore, diseases of the liver such as hepatitis, cirrhosis, and acute yellow atrophy (i.e., degeneration of the liver caused by toxins, infections, or other agents) can sometimes depress the clotting system so greatly that the patient experiences the development of a severe tendency to bleed. Another cause of depressed formation of clotting factors by the liver is vitamin K deficiency. Vitamin K is an essential factor to a liver carboxylase that adds a carboxyl group to glutamic acid residues on five of the important clotting factors: prothrombin, Factor VII, Factor IX, Factor X, and protein C. Upon adding the carboxyl group to glutamic acid residues on the immature clotting factors, vitamin K is oxidized and becomes inactive. Decreased Prothrombin, Factor VII, Factor IX, and Factor X Caused by Vitamin K PAGE 74 Deficiency In the absence of active vitamin K, subsequent insufficiency of these coagulation factors in the blood can lead to serious bleeding tendencies. Vitamin K is continually synthesized in the intestinal tract by bacteria, so vitamin K deficiency seldom occurs in healthy persons as a result of the absence of vitamin K from the diet (except in neonates before they establish their intestinal bacterial flora). However, in persons with gastrointestinal disease, vitamin K deficiency often occurs as a result of poor absorption of fats from the gastrointestinal tract because vitamin K is fat soluble and is ordinarily absorbed into the blood along with the fats.  PAGE 75 Hemophilia Hemophilia is a bleeding disease that occurs almost exclusively in males. In 85 percent of cases, it is caused by an abnormality or deficiency of Factor VIII ; this type of hemophilia is called hemophilia A or classic hemophilia. About 1 of every 10,000 males in the United States has classic hemophilia. In the other 15 percent of patients with hemophilia, the bleeding tendency is caused by deficiency of Factor IX. Both of these factors are transmitted genetically by way of the female chromosome. Therefore, a woman will almost never have hemophilia because at least one of her two X chromosomes will have the appropriate genes. If one of her X chromosomes is deficient, she will be a hemophilia carrier, transmitting the disease to half of her male offspring and transmitting the carrier state to half of her female offspring.  PAGE 76 Hemophilia The bleeding trait in hemophilia can have various degrees of severity, depending on the genetic deficiency. Bleeding usually does not occur except after trauma, but in some patients, the degree of trauma required to cause severe and prolonged bleeding may be so mild that it is hardly noticeable. For instance, bleeding can often last for days after extraction of a tooth.  PAGE 77 Thrombocytopenia Thrombocytopenia means the presence of very low numbers of platelets in the circulating blood. People with thrombocytopenia have a tendency to bleed, as do hemophiliacs, except that the bleeding is usually from many small venules or capillaries, rather than from larger vessels, as in hemophilia. As a result, small punctate hemorrhages occur throughout all the body tissues. The skin of such a person displays many small, purplish blotches, giving the disease the name thrombocytopenic purpura. As stated earlier, platelets are especially important for repair of minute breaks in capillaries and other small vessels.  PAGE 78 Thrombocytopenia Ordinarily, bleeding will not occur until the number of platelets in the blood falls below 50,000/µl, rather than the normal 150,000 to 300,000. Levels as low as 10,000/µl are frequently lethal. Even without making specific platelet counts in the blood, sometimes one can suspect the existence of thrombocytopenia if the person's blood clot fails to retract. As pointed out earlier, clot retraction is normally dependent on release of multiple coagulation factors from the large numbers of platelets entrapped in the fibrin mesh of the clot.  PAGE 79 Thrombocytopenia Most people with thrombocytopenia have the disease known as idiopathic thrombocytopenia, which means thrombocytopenia of unknown cause. In most of these people, it has been discovered that, for unknown reasons, specific antibodies have formed and react against the platelets to destroy them. Relief from bleeding for 1 to 4 days can often be effected in a patient with thrombocytopenia by giving fresh whole blood transfusions that contain large numbers of platelets. Also, splenectomy is often helpful, sometimes effecting almost complete cure because the spleen normally removes large numbers of platelets from the blood.  PAGE 80 Thromboembolic Conditions. Thrombi and Emboli An abnormal clot that develops in a blood vessel is called a thrombus. Once a clot has developed, continued flow of blood past the clot is likely to break it away from its attachment and cause the clot to flow with the blood; such freely flowing clots are known as emboli. Also, emboli that originate in large arteries or in the left side of the heart can flow peripherally and plug arteries or arterioles in the brain, kidneys, or elsewhere. Emboli that originate in the venous system or in the right side of the heart generally flow into the lungs to cause pulmonary arterial embolism.  PAGE 81 Cause of Thromboembolic Conditions The causes of thromboembolic conditions in the human being are usually twofold: (1) A roughened endothelial surface of a vessel —as may be caused by arteriosclerosis, infection, or trauma— is likely to initiate the clotting process, and (2) blood often clots when it flows very slowly through blood vessels, where small quantities of thrombin and other procoagulants are always being formed.  PAGE 82 Use of t-PA in Treating Intravascular Clots Genetically engineered t-PA is available. When delivered through a catheter to an area with a thrombus, it is effective in activating plasminogen to plasmin, which in turn can dissolve some intravascular clots. For instance, if used within the first hour or so after thrombotic occlusion of a coronary artery, the heart is often spared serious damage.  PAGE 83 Femoral Venous Thrombosis and Massive Pulmonary Embolism Because clotting almost always occurs when blood flow is blocked for many hours in any vessel of the body, the immobility of patients confined to bed plus the practice of propping the knees with pillows often causes intravascular clotting because of blood stasis in one or more of the leg veins for hours at a time. Then the clot grows, mainly in the direction of the slowly moving venous blood, sometimes growing the entire length of the leg veins and occasionally even up into the common iliac vein and inferior vena cava. Then, about 1 out of every 10 times, a large part of the clot disengages from its attachments to the vessel wall and flows freely with the venous blood through the right side of the heart and into the pulmonary arteries to cause massive blockage of the pulmonary arteries, called massive pulmonary embolism. If the clot is large enough to occlude both pulmonary arteries at the same time, immediate death ensues. If only one pulmonary artery is blocked, death may not occur, or the embolism may lead to death a few hours to several days later because of further growth of the clot within the pulmonary vessels. However, again, t-PA therapy can be a lifesaver.  PAGE 84 Disseminated Intravascular Coagulation Occasionally the clotting mechanism becomes activated in widespread areas of the circulation, giving rise to the condition called disseminated intravascular coagulation. This condition often results from the presence of large amounts of traumatized or dying tissue in the body that releases great quantities of tissue factor into the blood. Frequently, the clots are small but numerous, and they plug a large share of the small peripheral blood vessels. This process occurs especially in patients with widespread septicemia , in which either circulating bacteria or bacterial toxins—especially endotoxins —activate the clotting mechanisms.  PAGE 85 Disseminated Intravascular Coagulation Plugging of small peripheral vessels greatly diminishes delivery of oxygen and other nutrients to the tissues, a situation that leads to or exacerbates circulatory shock. It is partly for this reason that septicemic shock is lethal in 85 percent or more of patients. A peculiar effect of disseminated intravascular coagulation is that the patient on occasion begins to bleed. The reason for this bleeding is that so many of the clotting factors are removed by the widespread clotting that too few procoagulants remain to allow normal hemostasis of the remaining blood.  PAGE 86 Anticoagulants for Clinical Use. Heparin as an Intravenous Anticoagulant In some thromboembolic conditions, it is desirable to delay the coagulation process. Various anticoagulants have been developed for this purpose. The ones most useful clinically are heparin and the coumarins. Commercial heparin is extracted from several different animal tissues and prepared in almost pure form. Injection of relatively small quantities, about 0.5 to 1 mg/kg of body weight, causes the blood-clotting time to increase from a normal of about 6 minutes to 30 or more minutes. Furthermore, this change in clotting time occurs instantaneously, thereby immediately preventing or slowing further development of a thromboembolic condition. The action of heparin lasts about 1.5 to 4 hours. The injected heparin is destroyed by an enzyme in the blood known as heparinase.  PAGE 87 Haemostasis and thrombosis Ritter, James M., DPhil FRCP HonFBPhS FMedSci, Rang & Dale's Pharmacology, 23, 322-336.e2 Action of heparins. The schematic shows interactions of heparins, antithrombin III (AT III) and clotting factors. To increase the inactivation of thrombin (IIa) by AT III, heparin needs to interact with both substances (top), but to speed up its e... Copyright © 2024 Copyright © 2024 by Elsevier, Ltd. All rights reserved.  PAGE 88 Anticoagulants for Clinical Use. Coumarins as Anticoagulants When a coumarin, such as warfarin, is given to a patient, the amounts of active prothrombin and Factors VII, IX, and X, all formed by the liver, begin to fall. Warfarin causes this effect by inhibiting the enzyme VKOR c1. As discussed previously, this enzyme converts the inactive, oxidized form of vitamin K to its active, reduced form. By inhibiting VKOR c1, warfarin decreases the available active form of vitamin K in the tissues. When this decrease occurs, the coagulation factors are no longer carboxylated and are biologically inactive. Over several days the body stores of the active coagulation factors degrade and are replaced by inactive factors. Although the coagulation factors continue to be produced, they have greatly decreased coagulant activity.  PAGE 89 Anticoagulants for Clinical Use. Coumarins as Anticoagulants After administration of an effective dose of warfarin, the coagulant activity of the blood decreases to about 50 percent of normal by the end of 12 hours and to about 20 percent of normal by the end of 24 hours. In other words, the coagulation process is not blocked immediately but must await the degradation of the active prothrombin and the other affected coagulation factors already present in the plasma. Normal coagulation usually returns 1 to 3 days after discontinuing coumarin therapy.  PAGE 90 Haemostasis Waller, Derek G., Medical Pharmacology and Therapeutics, Chapter 11, 175-191 The coagulation cascade and the sites of action of anticoagulants. Roman numerals (e.g. ‘XI’) indicate the individual clotting factors in the cascade, with ‘XIa’ indicating the activated form. Contact with subendothelial collagen induces conformat... Copyright © 2022 Copyright © 2022 by Elsevier Ltd. All rights reserved.