Which of the following statements is correct regarding Nuclear Magnetic Resonance? 1) Derives protein structures in solution by determining pairwise distances of protons. 2) Is a m... Which of the following statements is correct regarding Nuclear Magnetic Resonance? 1) Derives protein structures in solution by determining pairwise distances of protons. 2) Is a method for measuring the chemical environment of a nucleus and how it changes upon ligand binding. 3) Allows determination of protein flexibility with atomic resolution. 4) Allows the structure determination of insoluble fibrillar proteins. 5) All of the above.
Understand the Problem
The question asks to identify the correct statement (or statements) about Nuclear Magnetic Resonance (NMR). This requires evaluating each statement's accuracy regarding NMR's capabilities and applications.
Answer
All of the above
All of the above. NMR determines protein structures in solution by measuring proton distances, assesses the chemical environment of a nucleus and its changes upon ligand binding, determines protein flexibility at atomic resolution, and helps determine the structure of insoluble fibrillar proteins.
Answer for screen readers
All of the above. NMR determines protein structures in solution by measuring proton distances, assesses the chemical environment of a nucleus and its changes upon ligand binding, determines protein flexibility at atomic resolution, and helps determine the structure of insoluble fibrillar proteins.
More Information
NMR spectroscopy is a versatile technique used to study the structure, dynamics, and interactions of biological macromolecules at atomic resolution. It can be applied to proteins in various states, including soluble and insoluble forms.
Tips
It's easy to focus on one aspect of NMR and miss its broader applications. Remember that NMR is not limited to soluble proteins or just structure determination; it provides insights into dynamics, interactions, and chemical environments as well.
Sources
- An Introduction to Biological NMR Spectroscopy - PMC - pmc.ncbi.nlm.nih.gov
- Structure Determination Nuclear Magnetic Resonance - an overview - sciencedirect.com
- NMR-Based Methods for Protein Analysis | Analytical Chemistry - pubs.acs.org
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