Which of the following conditions would likely enhance the separation of ionic drugs? A) Increasing ionic strength of buffers B) Decreasing temperature C) Using non-polar organic s... Which of the following conditions would likely enhance the separation of ionic drugs? A) Increasing ionic strength of buffers B) Decreasing temperature C) Using non-polar organic solvents D) Lowering pH
Understand the Problem
The question is asking which conditions would improve the separation of ionic drugs, suggesting that the reader needs to evaluate the effects of different factors such as ionic strength, temperature, solvent type, and pH on ionic drug separation.
Answer
Lowering pH
Lowering pH would likely enhance the separation of ionic drugs.
Answer for screen readers
Lowering pH would likely enhance the separation of ionic drugs.
More Information
Adjusting the pH of the mobile phase in liquid chromatography can impact the ionization state of the drug, influencing its interaction with the stationary phase and thus enhancing separation.
Tips
A common mistake is not considering the drug's pKa value when adjusting pH for its separation. Understanding how ionization affects retention can aid in better experimental design.
Sources
- Gradient elution of ionic compounds - Thermo Fisher Scientific - assets.thermofisher.com
- Chiral Chromatography Frequently Asked Questions - Sigma-Aldrich - sigmaaldrich.com
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