Which molecule, NADH or NAD+, absorbs a wavelength at 340 nm, thus making absorbance values lower?
Understand the Problem
The question is asking which molecule, NADH or NAD+, absorbs light at a wavelength of 340 nm and how this affects the absorbance values. This requires an understanding of the properties of these molecules related to spectroscopy.
Answer
NADH absorbs light at 340 nm, while NAD+ does not.
NADH absorbs light at 340 nm, while NAD+ does not.
Answer for screen readers
NADH absorbs light at 340 nm, while NAD+ does not.
More Information
The absorbance at 340 nm is commonly used in biochemical assays involving NADH because of this specific absorption characteristic, distinguishing NADH from NAD+.
Tips
A common mistake is assuming that both NAD+ and NADH absorb at 340 nm because both are related cofactors in biochemical reactions, but in fact, only NADH has significant absorbance at this wavelength.
Sources
- Towards the understanding of the absorption spectra of NAD(P)H ... - sciencedirect.com
- Determining NADH concentration - Agilent - agilent.com
- Why does the absorbency at 340nm decrease without enzyme and ... - researchgate.net
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