Which factor, when altered in the Antiglobulin Test (AGT), would most likely lead to a false-positive result due to non-specific aggregation?
Understand the Problem
The question is asking about potential causes of false-positive results in the Antiglobulin Test (AGT), specifically focusing on non-specific aggregation. It presents four possible factors related to the AGT procedure and asks which is most likely to lead to this type of error.
Answer
High protein concentrations.
High protein concentrations can cause false positive results in the Antiglobulin Test (AGT) due to non-specific aggregation.
Answer for screen readers
High protein concentrations can cause false positive results in the Antiglobulin Test (AGT) due to non-specific aggregation.
More Information
The antiglobulin test (AGT), also known as the Coombs test, detects antibodies that are stuck to the surface of red blood cells. A false positive result can occur due to factors like high protein concentrations, which cause cells to clump together nonspecifically.
Tips
To avoid false positives, ensure proper washing techniques are used to remove any unbound proteins or antibodies before adding the antiglobulin reagent.
Sources
- Coombs Test - StatPearls - NCBI Bookshelf - ncbi.nlm.nih.gov
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