Using gel-like chromatography, you aim to get the crude state of proteins. The proteins are given below. In order, state how they come out.
Understand the Problem
The question is asking how the proteins would be separated based on their characteristics in a gel-like chromatography experiment. The user is required to arrange the proteins in order according to their net charge and molecular weight as given in the table.
Answer
A, B, D, C, E
The order in which the proteins come out is A, B, D, C, E.
Answer for screen readers
The order in which the proteins come out is A, B, D, C, E.
More Information
In gel-filtration chromatography, size is the key determinant for separation, where larger proteins elute before smaller ones due to their inability to enter the pores of the gel.
Tips
A common mistake is to assume smaller proteins elute first, but in gel filtration, larger proteins pass through faster.
Sources
- Gel-Filtration Chromatography - PMC - pmc.ncbi.nlm.nih.gov
- Chapter 3: Investigating Proteins - Chemistry - wou.edu
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