Kinetics of immobilised enzyme
Understand the Problem
The question is asking about the kinetics of immobilized enzymes, which refers to the study of the rates of enzyme reactions when the enzymes are fixed or held in place, rather than free in solution. This involves understanding how factors like substrate concentration, temperature, and the nature of the support material affect enzyme activity.
Answer
Immobilization affects kinetic constants like Km and Vmax.
Immobilization can alter the kinetic constants such as Km and Vmax, affecting the enzyme's activity and intrinsic specificity.
Answer for screen readers
Immobilization can alter the kinetic constants such as Km and Vmax, affecting the enzyme's activity and intrinsic specificity.
More Information
Upon immobilization, enzymes may undergo internal structural changes and have restricted access to their active sites, which can modify their kinetic behaviors compared to when they are in solution. This can lead to changes in how efficiently the enzyme catalyzes reactions.
Tips
Ensure to differentiate between changes in constraints affecting enzyme kinetics due to immobilization as opposed to changes merely due to varying conditions such as pH or temperature.
Sources
- The web page with info on - Example Source - dbt.univr.it
- Kinetics of immobilised enzymes - Water structure and science - water.lsbu.ac.uk
