In UV-Vis spectrophotometry, what is the primary consequence of stray light within the instrument?
Understand the Problem
The question asks about the primary consequence of stray light in UV-Vis spectrophotometry. Stray light refers to any unwanted light that reaches the detector without passing through the sample. We need to identify the main problem this causes in spectroscopic measurements.
Answer
Stray light primarily reduces the observed peak height in UV-Vis spectrophotometry and can cause deviations from Beer-Lambert Law.
The primary consequence of stray light in UV-Vis spectrophotometry is the reduction of the observed peak height in the spectrum. It can also cause deviations from Beer-Lambert Law, leading to inaccurate absorption measurements.
Answer for screen readers
The primary consequence of stray light in UV-Vis spectrophotometry is the reduction of the observed peak height in the spectrum. It can also cause deviations from Beer-Lambert Law, leading to inaccurate absorption measurements.
More Information
Stray light refers to any unwanted light that reaches the detector in a spectrophotometer. Sources of stray light can include reflections and scattering within the instrument. High levels of stray light can significantly distort the measured spectra.
Tips
A common mistake is to assume stray light only affects absorbance values at high concentrations; it impacts the entire spectrum, especially peak heights.
Sources
- What is stray light? - Shimadzu Scientific Instruments - ssi.shimadzu.com
- Stray light in UV-Vis Absorbance measurements - chegg.com
- Stray light will cause apparent negative deviations from Beer's law - Science Direct - sciencedirect.com
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