If a tRNA molecule with the anticodon sequence 3'-UAC-5' was mutated to have the sequence 3'-UAG-5', what would be the most likely consequence?
Understand the Problem
The question asks about the consequence of a mutation in the anticodon sequence of a tRNA molecule. Specifically, if the anticodon 3'-UAC-5' mutates to 3'-UAG-5', we need to determine the most likely outcome regarding its ability to bind to mRNA codons and its role in translation.
Answer
Premature termination of translation.
The mutated tRNA would likely recognize a stop codon, leading to premature termination of translation.
Answer for screen readers
The mutated tRNA would likely recognize a stop codon, leading to premature termination of translation.
More Information
The original tRNA with the anticodon 3'-UAC-5' would bind to the mRNA codon 5'-AUG-3', which codes for methionine. However, if the anticodon is mutated to 3'-UAG-5', it will now bind to the mRNA codon 5'-AUU-3', which is a stop codon. Stop codons signal the end of translation, causing the ribosome to detach from the mRNA and release the polypeptide chain. This would result in a truncated protein.
Tips
A common mistake is failing to consider the directionality of the sequences (5' to 3') and how tRNA anticodons pair with mRNA codons. Also, remember that mutations can change the function of a tRNA, leading to the incorporation of the wrong amino acid or, in this case, premature termination.
Sources
- The genetic code & codon table (article) - Khan Academy - khanacademy.org
- The tRNA anticodon 3-UAC-5 will pair with the mRNA codon 5-AUU ... - toppr.com
AI-generated content may contain errors. Please verify critical information
