How does the Mediator induce the formation of the pre-initiation complex (PIC)?
Understand the Problem
The question is asking about the role of the Mediator in the formation of the pre-initiation complex (PIC), specifically how it influences the process through various proposed mechanisms involving enhancers, chromatin loops, RNA Polymerase II, and nucleosome assembly.
Answer
Mediator interacts with activator proteins and RNA Polymerase II to assemble the PIC with general transcription factors.
The Mediator complex induces the formation of the pre-initiation complex (PIC) by interacting with transcriptional activator proteins and directly contacting RNA Polymerase II. This assembly involves general transcription factors, facilitating the cooperative assembly of the PIC necessary for transcription initiation.
Answer for screen readers
The Mediator complex induces the formation of the pre-initiation complex (PIC) by interacting with transcriptional activator proteins and directly contacting RNA Polymerase II. This assembly involves general transcription factors, facilitating the cooperative assembly of the PIC necessary for transcription initiation.
More Information
The Mediator complex is a critical component in the regulation of gene transcription as it bridges transcriptional activators with the basal transcription machinery, thereby enhancing the efficiency of PIC formation. The interaction with RNA Polymerase II and transcription factors is vital for the initiation of transcription.
Tips
A common mistake is overlooking the role of various general transcription factors in the formation of the PIC. Each component, including TFIIA, -B, -D, -E, -F, -H, and -S, plays a specific part in the assembly process.
Sources
- The web page with info on - Example Source - pmc.ncbi.nlm.nih.gov
- Role of the pre-initiation complex in Mediator recruitment and ... - eLife - elifesciences.org
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