Design a 5 bp forward and reverse primer for the following 20 bp sequence: 5'-TCTGGGAGTTCATCTGGACA-3'
Understand the Problem
The question asks to design 5 base pair (bp) forward and reverse primers based on the provided 20 bp DNA sequence. Specifically, you need to identify the first 5 bases of the sequence for the forward primer. The reverse primer requires you to find the last 5 bases, then find the complement of this 5 base sequence, and reverse these complements to obtain the reverse primer sequence.
Answer
Forward primer: 5'-TCTGG-3', Reverse primer: 5'-TGACC-3'.
The 5bp forward primer is 5'-TCTGG-3', and the 5bp reverse primer is 5'-TGACC-3'.
Answer for screen readers
The 5bp forward primer is 5'-TCTGG-3', and the 5bp reverse primer is 5'-TGACC-3'.
More Information
Primers are short, single-stranded DNA sequences used in PCR to initiate DNA synthesis. The forward primer binds to the start of the target DNA sequence, and the reverse primer binds to the end of the target sequence on the opposite strand.
Tips
Ensure the reverse primer is synthesized in the reverse complementary direction relative to the template strand.
Sources
- Primer Design for PCR - YouTube - youtube.com
- Design primers for PCR from given DNA sequence - biology.stackexchange.com
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