Definitions related to different types of volumes with a chromatography column: Exclusion volume (Vo), Internal pore volume (Vi), Elution volume (Ve), and Total volume (Vt).
Understand the Problem
The image provides definitions for several terms related to chromatography, specifically focusing on different types of volumes within a chromatography column: Exclusion volume (Vo), Internal pore volume (Vi), Elution volume (Ve), and Total volume (Vt).
Answer
Here are the definitions: * **Exclusion volume (Vo):** volume between gel beads * **Internal pore volume (Vi):** volume inside the beads * **Elution volume (Ve):** volume required to elute a molecule * **Total volume (Vt):** total volume of mobile phase; Vt = Vi+Vo
Here are the definitions:
- Exclusion volume (Vo): volume between gel beads; Vo = elution volume of large molecules (that do not enter the pores)
- Internal pore volume (Vi): volume inside the beads; Vi = elution volume of small molecules
- Elution volume (Ve): volume required to elute a particular molecule
- Total volume (Vt): total volume of mobile phase in the column; Vt = Vi+Vo
Answer for screen readers
Here are the definitions:
- Exclusion volume (Vo): volume between gel beads; Vo = elution volume of large molecules (that do not enter the pores)
- Internal pore volume (Vi): volume inside the beads; Vi = elution volume of small molecules
- Elution volume (Ve): volume required to elute a particular molecule
- Total volume (Vt): total volume of mobile phase in the column; Vt = Vi+Vo
More Information
The different volumes are important parameters in chromatography, especially in size exclusion chromatography.
Tips
Pay attention to which molecules are eluted in which volume when solving chromatography problems.
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