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Questions and Answers

Which crystal is described as dissolving in acids without effervescence?

  • Calcium carbonate
  • Calcium oxalate (correct)
  • Sodium sulfate
  • Magnesium phosphate

What primary metabolite consists mainly of amylose and amylopectin?

  • Lipids
  • Carbohydrates (correct)
  • Nucleic acids
  • Proteins

Which method is used to identify primary metabolites?

  • Chromatographic techniques
  • Chemical tests for food storage products (correct)
  • Microscopic identification
  • Spectroscopy

What is a characteristic of calcium carbonate crystals?

<p>Can be found in the leaves of Cannabis sativa (D)</p> Signup and view all the answers

Which of the following compounds is classified as a secondary metabolite?

<p>Tannins (C)</p> Signup and view all the answers

What is the result of performing the Molisch test on starch?

<p>Violet ring appears (C)</p> Signup and view all the answers

How can starch be microscopically identified?

<p>By its size and shape (A)</p> Signup and view all the answers

What is the primary composition of fixed oils and fats in plants?

<p>Esters of fatty acids with glycerol (B)</p> Signup and view all the answers

Which reagent is used to test for proteins, resulting in a red color upon warming?

<p>Millon’s reagent (C)</p> Signup and view all the answers

Which of the following describes simple aggregation in starch granules?

<p>One hilum occupies all the leucoplast (D)</p> Signup and view all the answers

Flashcards

Molisch Test for Starch

A chemical test for starch using α-naphthol and concentrated sulfuric acid that produces a violet ring.

Fehling Test

A chemical test used to detect reducing sugars, which produce a reddish-brown precipitate when combined with Fehling's solution and a sample.

Starch Granule Identification

Identifying starch granules based on shape, size, hilum, striations, and aggregation patterns under a microscope.

Protein Storage in Plants

Plant proteins are stored in structures called aleurone grains.

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Millon's Reagent Test for Proteins

A test to detect proteins by producing a red color change when heated with Millon's reagent.

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Calcium Oxalate Crystals

Insoluble crystals found in plants, often in various shapes (raphides, prisms). Important for plant identification.

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Primary Metabolites

Essential molecules for plant growth and function; includes sugars, proteins, and fats.

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Starch

A carbohydrate storage form in plants, composed of amylose and amylopectin, insoluble in water.

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Iodine Test for Starch

A chemical test that uses iodine to detect the presence of starch; starch turns blue with iodine.

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Calcium Carbonate Crystals

Insoluble crystals found in plants, sometimes in hairs; they dissolve in dilute acids with effervescence.

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Study Notes

Plant Cell Types & Contents

  • Plant cells are identified microscopically, chemically, and physically.
  • Physical identification includes cell membrane, cytoplasm, nucleus, and organelles.
  • Chemical identification uses plant metabolites like primary and secondary metabolites.
  • Microscopic identification includes crystals like calcium oxalate and calcium carbonate.

Calcium Oxalate

  • Calcium oxalate crystals are insoluble in water, glycerin, alcohol, chloral hydrate and caustic alkalis.
  • They dissolve in acids without effervescence.
  • Types include prisms, crystal sheath (e.g., Senna), clusters, crystal layers (e.g., Rhubarb, Datura), and Raphides/acicular crystals (e.g., Squill, Ipecacuanha).
  • Some examples of plants containing calcium oxalate crystals are Senna, Rhubarb, Datura, and Belladonna.

Calcium Carbonate

  • Calcium carbonate (cystolith) is found in hairs, like those of Cannabis sativa.
  • It dissolves in dilute acids with effervescence,.

Plant Metabolites

  • Primary Metabolites (Food Storage Products): Carbohydrates, proteins, and lipids
  • Secondary Metabolites: By-products of metabolism, including alkaloids, glycosides, volatile oils, tannins, resins, and more.

Carbohydrates

  • Occur in plants as granules (amylose and amylopectin).
  • Amylose forms 20% of starch, and amylopectin makes about 80%.
  • Starch is insoluble in water but turns blue with iodine.
  • Uses include itching remedies, iodine poisoning, lubricants, tablet disintegrants and barium meal suspending agents.

Protein

  • Proteins are nitrogenous organic compounds, polymers of L-amino acids.
  • Stored in plants as aleurone grains.
  • Proteins are microscopically identified using Million's reagent which stains proteins red on warming.

Fixed Oils and Fats

  • Found in various plant parts, especially seeds
  • Chemically, they are mixtures of fatty acid esters with glycerol.
  • These components are hydrolyzed by aqueous alkalis, producing soaps and glycerin.
  • Fats and oils are greasy, non-volatile at room temperature, and are lighter than water and insoluble in it.
  • They can be detected microscopically by the Oil Red O (ORO) stain, a fat-soluble dye that stains neutral lipids, cholesteryl esters, and lipoproteins.

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