Ziehl-Neelsen Staining Technique
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Questions and Answers

Who first developed the differential staining technique later modified by Neelsen?

  • Loeffler
  • Koch
  • Gram
  • Ziehl (correct)

What is the alternative name for the Ziehl-Neelsen staining technique?

  • Neelsen staining
  • Carbol-fuchsin staining
  • Acid-fast staining (correct)
  • Gram staining

What year did Neelsen use Ziehl’s carbol-fuchsin and heat in the staining process?

  • 1883 (correct)
  • 1873
  • 1903
  • 1893

What substance is used to decolorize in the Ziehl-Neelsen staining technique?

<p>Acid alcohol (D)</p> Signup and view all the answers

Which dye is used as a counter stain in the Ziehl-Neelsen staining technique?

<p>Methylene blue (C)</p> Signup and view all the answers

Study Notes

Differential Staining Techniques

  • The Ziehl-Neelsen staining technique is a differential staining technique that was first developed by Ziehl and later modified by Neelsen.
  • The technique was developed in 1883 when Neelsen used Ziehl's carbol-fuchsin and heat, followed by decolorization with acid alcohol and counter-staining with methylene blue.

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Description

Learn about the differential staining technique developed by Ziehl and modified by Neelsen, used to identify microorganisms.

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