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Questions and Answers

What is the primary role of integral proteins in the red blood cell membrane?

  • Facilitating glucose metabolism
  • Providing structural rigidity
  • Manufacturing hemoglobin
  • Transporting substances across the membrane (correct)
  • Which statement best describes the function of glycophorins in red blood cells?

  • They are involved in blood group antigen expression. (correct)
  • They promote glucose uptake in the cell.
  • They play a key role in hemoglobin synthesis.
  • They are responsible for the biconcave shape of erythrocytes.
  • What is the significance of the zeta potential in red blood cells?

  • It regulates the oxygen-carrying capacity of the cells.
  • It determines cell shape and morphology.
  • It affects the stability of hemoglobin.
  • It influences the aggregation of red blood cells. (correct)
  • Which component forms the majority of the membrane composition of red blood cells?

    <p>Proteins (A)</p> Signup and view all the answers

    How does the biconcave shape of a red blood cell contribute to its function?

    <p>Increases surface area for gas exchange (A)</p> Signup and view all the answers

    What is a major element of the red blood cell’s phospholipid bilayer?

    <p>Phosphate heads (A)</p> Signup and view all the answers

    Which of the following accurately describes the average size of red blood cells?

    <p>6-8 um (B)</p> Signup and view all the answers

    What characteristic contributes to the salmon pink color of red blood cells?

    <p>Heme portion of hemoglobin (B)</p> Signup and view all the answers

    What is one primary role of integral proteins found in the RBC membrane?

    <p>To act as channels for the diffusion of ions and molecules (A)</p> Signup and view all the answers

    How does sialic acid contribute to the properties of RBCs?

    <p>By creating a zeta potential that leads to negative charge (B)</p> Signup and view all the answers

    Which function is NOT associated with glycophorins in the RBC membrane?

    <p>Facilitating the transport of oxygen across the membrane (D)</p> Signup and view all the answers

    What is true regarding the deformability of RBCs?

    <p>It enhances their ability to slip through narrow blood vessels (B)</p> Signup and view all the answers

    What characteristic of sialic acid contributes to the negative charge of RBCs?

    <p>The formation of a cloud of negatively charged particles (B)</p> Signup and view all the answers

    Which type of proteins serve as essential adhesion sites in the RBC membrane?

    <p>Integral adhesion proteins (D)</p> Signup and view all the answers

    Which aspect of the phospholipid bilayer is essential for the movement of integral proteins?

    <p>The fluidity and flexibility of the bilayer (A)</p> Signup and view all the answers

    What do integral proteins and glycoproteins have in common regarding their functions in RBCs?

    <p>Both are involved in cell signaling and transport (B)</p> Signup and view all the answers

    Which statement best describes the significance of the zeta potential in red blood cells?

    <p>It minimizes cell aggregation during circulation (B)</p> Signup and view all the answers

    Which statement correctly describes integral proteins in the RBC membrane?

    <p>They traverse the entire phospholipid bilayer, connecting the interior and exterior. (D)</p> Signup and view all the answers

    What distinguishes peripheral proteins from integral proteins in the RBC membrane?

    <p>Peripheral proteins do not penetrate the bilayer but associate with its surfaces. (C)</p> Signup and view all the answers

    What is a key function of glycophorin in red blood cells?

    <p>Serves as a receptor for antibodies. (A)</p> Signup and view all the answers

    Which of the following statements about zeta potential in red blood cells is accurate?

    <p>Zeta potential influences the stability of RBC suspensions. (C)</p> Signup and view all the answers

    Which statement correctly identifies the contribution of adhesion proteins in RBCs?

    <p>They enable interactions between RBCs and vessel walls. (D)</p> Signup and view all the answers

    What is a primary effect of hereditary spherocytosis on red blood cells?

    <p>Altered shape leading to increased fragility and hemolysis. (B)</p> Signup and view all the answers

    Why is Phosphatidylserine significant in the context of red blood cell membranes?

    <p>It plays a crucial role in cell signaling during apoptosis. (C)</p> Signup and view all the answers

    Which phospholipid is an exception to the rule of 'Phosphatidyl' being located in the inner layer?

    <p>Phosphatidylcholine. (A)</p> Signup and view all the answers

    Flashcards

    Peripheral proteins

    Proteins that are located on the surface of the cell membrane, not penetrating the entire phospholipid bilayer.

    Integral proteins

    Proteins that span the entire phospholipid bilayer, extending into both the inner and outer layers.

    Phospholipid bilayer

    A critical membrane structure composed of two layers of phospholipid molecules.

    Phosphatidylcholine

    A type of phospholipid typically found in the outer layer of the cell membrane.

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    Phosphatidylinositol

    A type of phospholipid typically found in the inner layer of the cell membrane.

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    Phosphatidylserine

    A negatively charged phospholipid that can relocate to the outer layer, found in the inner layer.

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    Skeletal protein

    Proteins that maintain the structure of red blood cells.

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    Hereditary spherocytosis

    A genetic disorder affecting red blood cell shape, often linked to mutations in integral membrane proteins.

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    Red Blood Cell Size

    Red blood cells (RBCs) have an average size of 6-8 micrometers.

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    RBC Shape

    Red blood cells are biconcave discs, resembling a donut with a central concavity.

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    RBC Surface Area

    The average surface area of a red blood cell is 140 square micrometers.

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    RBC Color

    Red blood cells appear salmon pink due to Hemoglobin.

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    Hemoglobin Location in RBC

    Hemoglobin is more concentrated on the outer edges of the red blood cell

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    Membrane Composition

    Red blood cell membranes are mostly comprised of proteins, followed by lipids and carbohydrates.

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    Protein Percentage in RBC Membrane

    About 50% of the red blood cell membrane is comprised of protein.

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    Transmembrane Proteins

    Another name for integral proteins, emphasizing their passage through the entire cell membrane.

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    RBC Membrane

    The membrane surrounding red blood cells (RBCs), a specialized type of cell in our blood.

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    Deformability

    The ability of red blood cells to change shape, crucial for their passage through narrow blood vessels.

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    Sialic Acid

    A molecule found on the cell membrane's surface, particularly on red blood cells, which contributes to a negative charge.

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    Zeta Potential

    The electrical potential difference between the cell and its surrounding fluid, which can be affected by molecules like sialic acid, creating a negative charge cloud surrounding the cells.

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    Blood Group Antigens

    Carbohydrate-based molecules defining different blood groups (e.g., ABO, MNSS).

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    Transport and Adhesion

    Integral proteins can serve as portals for substances moving in and out of cells or facilitating interactions between cells.

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    Study Notes

    Red Blood Cell Morphology

    • Size: 6-8 μm
    • Color: Salmon pink due to heme portion of hemoglobin
    • Shape: Biconcave disc (discocyte)
    • Average surface area: 140 μm²

    Red Blood Cell Membrane Composition

    • 50% protein (Rodak: 52%)
    • 40% lipids
    • 10% carbohydrates
    • Phospholipid bilayer: Composed of phosphate heads and fatty acid tails
    • Cholesterol: Maintains membrane fluidity and tensile strength
    • Integral proteins: Span the membrane, act as transporters, adhesion sites and receptors
    • Peripheral proteins: Located on the inner surface of the membrane, involved in maintaining the cell's shape
    • Glycophorins: Integral proteins with attached carbohydrates; important for negative charge (zeta potential) and blood group antigens (e.g., ABH, MNSS)
    • Sialic acid: Component of glycophorins, contributing to the negative membrane charge

    Red Blood Cell Deformability

    • Flexible shape allows them to bend and flex in narrow capillaries
    • Essential for efficient gas exchange

    Red Blood Cell Hereditary Disorders

    • Hereditary spherocytosis: Defect in integral proteins (e.g., band 3, ankyrin, protein 4.1, or spectrin) leading to spherical shape and premature destruction.
    • Hereditary elliptocytosis: Defect in peripheral proteins (e.g., spectrin) leading to elliptical shape and hemolysis

    Red Blood Cell Metabolism - Embden-Meyerhof (EMP) Pathway

    • Anaerobic glycolysis, primary energy source for red blood cells
    • Generates 2 ATP molecules per glucose molecule
    • Involves multiple enzymatic steps and intermediates, from glucose to pyruvate and ultimately lactate.

    Red Blood Cell Metabolism - Hexose Monophosphate (HMP) Shunt

    • Alternative pathway for glucose metabolism
    • NADPH generation for reducing oxidative stress
    • Maintains the reduced form of glutathione, vital for protecting hemoglobin
    • Important for preserving and preventing oxidative damage to erythrocytes

    Red Blood Cell Metabolism - Methemoglobin Reductase Pathway

    • Keeps hemoglobin in its reduced ferrous state (Fe2+)
    • Essential for oxygen transport
    • Maintains oxygen-binding capacity

    Red Blood Cell Metabolism - Rapoport-Luebering (RL) Pathway

    • Generates 2,3-BPG (bisphosphoglycerate), a regulatory molecule
    • Lowers hemoglobin's oxygen affinity, facilitating oxygen release in tissues
    • Shifts the oxygen dissociation curve to the right

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