WK2- Immunofluorescence and Confocal Microscopy

The scattering of light as it passes through a substance

The direct absorption of light by a sample without emission

The reflection of light off a sample to create an image

The emission of light by a substance that has absorbed light or other electromagnetic radiation This is the correct answer. Fluorescence occurs when a fluorophore absorbs light at one wavelength and emits light at a longer wavelength. (correct)

A method for measuring the pH of solutions

A type of microscopy used primarily for electron imaging

A technique used to visualize proteins in cells using fluorescent antibodies (correct)

A technique used for DNA sequencing

<p>Laser = Provides focused light for illumination Pinhole = Enhances optical sectioning by excluding out-of-focus light Detector = Collects emitted fluorescence from the sample Computer = Processes and reconstructs images for analysis</p> Signup and view all the answers

<p>Absorption of energy results in excitation of the compound from S0 to S1.</p> Signup and view all the answers