WK2- Immunofluorescence and Confocal Microscopy

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Questions and Answers

what is fluroscence?

The scattering of light as it passes through a substance
The direct absorption of light by a sample without emission
The reflection of light off a sample to create an image
The emission of light by a substance that has absorbed light or other electromagnetic radiation This is the correct answer. Fluorescence occurs when a fluorophore absorbs light at one wavelength and emits light at a longer wavelength. (correct)

What is IFM (Immunofluorescence Microscopy)?

A method for measuring the pH of solutions
A type of microscopy used primarily for electron imaging
A technique used to visualize proteins in cells using fluorescent antibodies (correct)
A technique used for DNA sequencing

Match the following components of confocal microscopy with their functions:

<p>Laser = Provides focused light for illumination Pinhole = Enhances optical sectioning by excluding out-of-focus light Detector = Collects emitted fluorescence from the sample Computer = Processes and reconstructs images for analysis</p> Signup and view all the answers