Podcast
Questions and Answers
What affects the strength of the bond between an antigen and a binding site?
What affects the strength of the bond between an antigen and a binding site?
- The temperature of the assay
- The size of the antigen
- The type of antibody used
- The resemblance of the cross-reacting antigen to the original antigen (correct)
How is affinity related to the sensitivity of an assay reaction?
How is affinity related to the sensitivity of an assay reaction?
- Higher affinity decreases sensitivity
- Affinity does not affect the sensitivity of an assay
- Higher affinity results in more visible complexes (correct)
- Higher affinity leads to fewer complexes formed
What is avidity in the context of immunology?
What is avidity in the context of immunology?
- The characteristic of an antibody to only bind one antigen
- The maximum temperature an assay can reach
- The strength of a single antibody's bond to an antigen
- The overall binding strength of multivalent antibodies to multivalent antigens (correct)
Which antibody type has the highest potential binding sites for antigens?
Which antibody type has the highest potential binding sites for antigens?
What does the law of mass action state in relation to antigen-antibody binding?
What does the law of mass action state in relation to antigen-antibody binding?
What mainly determines the affinity of an antibody to an antigen?
What mainly determines the affinity of an antibody to an antigen?
Which statement is true about the monomers in IgM?
Which statement is true about the monomers in IgM?
What does a higher equilibrium constant indicate in antigen-antibody reactions?
What does a higher equilibrium constant indicate in antigen-antibody reactions?
What common outcome can result from the interaction of pollens with IgE?
What common outcome can result from the interaction of pollens with IgE?
What is one characteristic of tertiary binding tests in immunology?
What is one characteristic of tertiary binding tests in immunology?
In which decade was the enzyme immunoassay (EIA) developed?
In which decade was the enzyme immunoassay (EIA) developed?
What is a major benefit of using enzymes in EIA?
What is a major benefit of using enzymes in EIA?
Which method is NOT typically associated with immunoassays?
Which method is NOT typically associated with immunoassays?
What is a result of precipitation immunoassays?
What is a result of precipitation immunoassays?
Which assessment is true about agglutination in immunoassays?
Which assessment is true about agglutination in immunoassays?
What was a major focus of research following the development of RIA?
What was a major focus of research following the development of RIA?
What are the two key characteristics of antibodies that influence antigen-antibody binding?
What are the two key characteristics of antibodies that influence antigen-antibody binding?
Which of the following correctly describes affinity in relation to antibody activity?
Which of the following correctly describes affinity in relation to antibody activity?
Which type of bond is NOT included in the noncovalent interactions that hold antigen-antibody complexes together?
Which type of bond is NOT included in the noncovalent interactions that hold antigen-antibody complexes together?
What term refers to the ability of an antibody to react with antigens that are structurally similar to the original antigen?
What term refers to the ability of an antibody to react with antigens that are structurally similar to the original antigen?
Which body fluid is primarily used in serological tests?
Which body fluid is primarily used in serological tests?
How is the stability of antigen-antibody complexes influenced?
How is the stability of antigen-antibody complexes influenced?
What range do the noncovalent interactions between antibodies and antigens primarily occur?
What range do the noncovalent interactions between antibodies and antigens primarily occur?
What is the primary purpose of immunoassays in the clinical laboratory?
What is the primary purpose of immunoassays in the clinical laboratory?
Which of the following is NOT a type of molecule that immunoassays can detect?
Which of the following is NOT a type of molecule that immunoassays can detect?
What characteristic is important for antigen-antibody binding to be stable?
What characteristic is important for antigen-antibody binding to be stable?
What is the primary purpose of using fluorophores in fluorescent immunoassays (FIA)?
What is the primary purpose of using fluorophores in fluorescent immunoassays (FIA)?
What type of particles are commonly used as carriers in fluorescent immunoassays?
What type of particles are commonly used as carriers in fluorescent immunoassays?
What was a key contribution of Yalow and Berson's development of radioimmunoassay (RIA) in 1959?
What was a key contribution of Yalow and Berson's development of radioimmunoassay (RIA) in 1959?
What can decrease the sensitivity of fluorescent immunoassays?
What can decrease the sensitivity of fluorescent immunoassays?
What improvement in fluorescent immunoassays has been noted due to the introduction of a new class of fluorescent compounds?
What improvement in fluorescent immunoassays has been noted due to the introduction of a new class of fluorescent compounds?
What is the primary concern with using background fluorescence in biological specimens during FIA?
What is the primary concern with using background fluorescence in biological specimens during FIA?
What concentration level can sophisticated instrumentation detect using fluorescent immunoassays?
What concentration level can sophisticated instrumentation detect using fluorescent immunoassays?
Which of the following statements about agglutination in FIA is true?
Which of the following statements about agglutination in FIA is true?
What does avidity measure in the context of antigen-antibody interactions?
What does avidity measure in the context of antigen-antibody interactions?
Why does IgM have a higher avidity compared to IgG?
Why does IgM have a higher avidity compared to IgG?
What is the equilibrium constant Keq indicative of?
What is the equilibrium constant Keq indicative of?
Which of the following is a characteristic of secondary reactions in antigen-antibody interactions?
Which of the following is a characteristic of secondary reactions in antigen-antibody interactions?
What distinguishes primary binding assays from secondary binding tests?
What distinguishes primary binding assays from secondary binding tests?
Which one of the following types of secondary reactions involves the clumping of particulate antigens?
Which one of the following types of secondary reactions involves the clumping of particulate antigens?
In the context of antigen-antibody interactions, what does the term contact area refer to?
In the context of antigen-antibody interactions, what does the term contact area refer to?
What primary function do labels serve in immunoassays?
What primary function do labels serve in immunoassays?
How does increasing avidity impact the formation of antigen-antibody complexes?
How does increasing avidity impact the formation of antigen-antibody complexes?
What typically occurs during tertiary reactions of antigen-antibody interactions?
What typically occurs during tertiary reactions of antigen-antibody interactions?
Which immunoassay uses radioisotopes as labels?
Which immunoassay uses radioisotopes as labels?
Which of the following best describes monovalent antibodies?
Which of the following best describes monovalent antibodies?
What is the effect of high affinity antibodies when used in conjunction with high avidity?
What is the effect of high affinity antibodies when used in conjunction with high avidity?
Flashcards are hidden until you start studying
