XLD Agar for Gram-Negative Bacteria

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Questions and Answers

What type of bacteria does XLD agar selectively isolate?

XLD agar selectively isolates Gram-negative bacteria.

Describe the colony appearance of Salmonella species on XLD agar.

Salmonella species appear as red colonies, some with black centers on XLD agar.

What color colonies are representative of Shigella species on XLD agar?

Shigella species produce red colonies on XLD agar.

How do coliform bacteria appear when cultured on XLD agar?

<p>Coliforms appear as yellow to orange colonies on XLD agar.</p> Signup and view all the answers

What are the characteristics of Pseudomonas aeruginosa colonies on XLD agar?

<p>Pseudomonas aeruginosa forms pink, flat, rough colonies on XLD agar.</p> Signup and view all the answers

XLD agar is selective for ______ bacteria.

<p>Gram-negative</p> Signup and view all the answers

Coliforms appear as ______ to orange colonies on XLD agar.

<p>yellow</p> Signup and view all the answers

Salmonella species typically form ______ colonies, some with black centers.

<p>red</p> Signup and view all the answers

Shigella species are identified by ______ colonies on XLD agar.

<p>red</p> Signup and view all the answers

Pseudomonas aeruginosa colonies are described as pink, flat, and ______.

<p>rough</p> Signup and view all the answers

Flashcards

XLD Agar

A selective agar medium specifically designed to isolate Gram-negative bacteria.

Salmonella colonies on XLD

Appear red, potentially with black centers.

Shigella colonies on XLD

Appear red on XLD agar.

Coliform colonies on XLD

Appear yellow to orange.

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Pseudomonas aeruginosa on XLD

Appear pink, flat, and rough.

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What makes XLD selective?

XLD agar contains ingredients like Xylose, Lysine, and Desoxycholate that inhibit the growth of most Gram-positive bacteria and some Gram-negative bacteria, allowing Salmonella and Shigella to thrive.

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Study Notes

XLD Agar: Selective Medium for Gram-Negative Bacteria

  • XLD agar is a selective medium specifically designed to isolate Gram-negative bacteria.
  • Follow the instructions on the agar bottle for proper procedure.
  • Primarily used to isolate Salmonella and Shigella from clinical specimens and food.
  • Salmonella: Exhibits red colonies, some with black centers.
  • Shigella: Displays red colonies.
  • Coliforms: Colonies range in color from yellow to orange.
  • Pseudomonas aeruginosa: Develops pink, flat, rough colonies.

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