Week 8 – Natural Biomaterials: Gelatin and Collagen Relationship
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Questions and Answers

What is a common issue associated with hydrogels mentioned in the text?

  • Lack of promoting angiogenesis
  • High stability in physiological conditions
  • Enhancing neurite extension
  • Mechanical instability (correct)

How are fibrin hydrogels formed according to the text?

  • Physical evaporation
  • Chemical precipitation
  • Enzymatic polymerization (correct)
  • Thermal condensation

What is the role of Metalloproteinases (MMPs) mentioned in the text?

  • Stimulating nerve regeneration
  • Breaking down proteins (correct)
  • Promoting blood supply
  • Enhancing cell growth

Which substances are used to control the rate of degradation in tissue engineering according to the text?

<p>Plasmin and aprotinin (D)</p> Signup and view all the answers

Why are Aprotinin and Tranexamic acid used in tissue engineering as mentioned in the text?

<p>To control the rate of degradation by inhibiting plasmin (C)</p> Signup and view all the answers

What is a key property of fibrin hydrogels that makes them desirable for tissue engineering according to the text?

<p>Capability to promote angiogenesis (A)</p> Signup and view all the answers

What determines the biophysical properties of gelatin gels?

<p>Crosslinking (D)</p> Signup and view all the answers

At what temperature does a reverse coil to triple helix transition occur in gelatin gels?

<p>30°C (D)</p> Signup and view all the answers

Why are chemical gels with glutaraldehyde preferred over natural methods?

<p>They are inexpensive and easily accessible (D)</p> Signup and view all the answers

How does pH affect gelatin gel strength in solution?

<p>Decreases gel strength (C)</p> Signup and view all the answers

Why do physical gelatin gels require crosslinkers for stability?

<p>To prevent the gel from falling apart easily (D)</p> Signup and view all the answers

What factor shows the most response in terms of gelation in gelatin gels?

<p>$pH 5.0$ (B)</p> Signup and view all the answers

What is gelatin?

<p>A form of irreversibly hydrolyzed collagen (D)</p> Signup and view all the answers

Which process is used to produce gelatin?

<p>Hydrolysis using heat and enzymes (A)</p> Signup and view all the answers

What gives gelatin its thermoresponsive properties?

<p>Random super coil formation upon hydrolysis (B)</p> Signup and view all the answers

Why is gelatin considered biocompatible?

<p>It does not induce toxicity and is less antigenic (B)</p> Signup and view all the answers

What makes gelatin highly soluble compared to other ECM proteins?

<p>Abundance of a specific amino acid sequence (D)</p> Signup and view all the answers

Why is intact protein not necessary for gelatin in tissue engineering applications?

<p>Only certain sections of the protein sequence are vital for cell attachment (B)</p> Signup and view all the answers

What is the cleavage site on the α chain?

<p>Gly-Pro-Arg (C)</p> Signup and view all the answers

Why is the removal of the 'A' peptide cleavage strictly required for fibrin polymerization?

<p>To improve the efficiency of polymerization (B)</p> Signup and view all the answers

What interaction links the center of a fibrin assembly with the ends of two other fibrin assemblies?

<p>Knob-hole binding (C)</p> Signup and view all the answers

What is the function of thrombin in fibrin polymerization?

<p>To remove fibrinopeptides (D)</p> Signup and view all the answers

What do knob-hole interactions lead to in fibrin polymerization?

<p>Formation of oligomers (A)</p> Signup and view all the answers

What covers the knobs that are complementary to holes in fibrin polymerization?

<p>/brinopeptides (C)</p> Signup and view all the answers

What are the major disadvantages of using a fibrin-based scaffold for tissue engineering purposes?

<p>Rapid degradation before proper tissue formation, squishing during sheet formation, and shrinkage of the gel (C)</p> Signup and view all the answers

Which method can help reduce the rapid degradation of fibrin-based scaffolds?

<p>Using inhibitors to prevent degradation (B)</p> Signup and view all the answers

Why might a fibrin-based scaffold encounter problems with shrinkage during tissue engineering?

<p>Excessive initial concentration of fibrinogen and calcium (A)</p> Signup and view all the answers

Which strategy can help address the possibility of squishing during sheet formation when using a fibrin-based scaffold?

<p>Utilizing methods to ensure proper entrapment (D)</p> Signup and view all the answers

What changes can be made to a fibrin-based scaffold to enhance its mechanical stiffness?

<p>Adjusting the initial concentration of fibrinogen and calcium (B)</p> Signup and view all the answers

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