10 Questions
Which staining method is suitable for acid fast stain, safranin, and chromotrope stain?
PVA
Why is PVA less suitable for concentration procedures?
It is difficult to prepare in the laboratory
Which fixative can interfere with polymerase chain reaction (PCR) if used for an extended fixation time?
Fixative containing iodine
What does iodine cause in relation to protozoa?
Distortion
Which staining method interferes with other stains and fluorescence?
Fixative containing iodine
Why is PVA considered good for the preservation of protozoan trophozoites and cysts?
It is easy to prepare
Which fixative is compatible with immunoassay kits for Giardia lamblia and Cryptosporodium spp?
Fixative containing iodine
Why is MIF suitable for field surveys?
It preserves organisms well
What issue arises when using a fixative that interferes with PCR after an extended fixation time?
Interference with immunoassay kits
Why is iodine not suitable for some permanent stained smears such as trichrome?
Iodine may cause distortion of protozoa
Test your knowledge on the modified Ritchie's concentration method procedure described by Weber et al. in 1992 for the concentration of oocysts from sediment samples. This quiz covers the centrifugation steps, resuspension process, and layering technique used in the procedure.
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