(Weber et al., 1992) Modified Ritchie’s Concentration Method Procedure Quiz
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Questions and Answers

Which staining method is suitable for acid fast stain, safranin, and chromotrope stain?

  • Merthiolateiodine-formaldehyde solution
  • Fixative containing iodine
  • MIF
  • PVA (correct)

Why is PVA less suitable for concentration procedures?

  • Preserved samples do not remain stable for long
  • It interferes with other stains
  • It contains mercuric chloride
  • It is difficult to prepare in the laboratory (correct)

Which fixative can interfere with polymerase chain reaction (PCR) if used for an extended fixation time?

  • Merthiolateiodine-formaldehyde solution
  • Fixative containing iodine (correct)
  • MIF
  • PVA

What does iodine cause in relation to protozoa?

<p>Distortion (D)</p> Signup and view all the answers

Which staining method interferes with other stains and fluorescence?

<p>Fixative containing iodine (D)</p> Signup and view all the answers

Why is PVA considered good for the preservation of protozoan trophozoites and cysts?

<p>It is easy to prepare (B)</p> Signup and view all the answers

Which fixative is compatible with immunoassay kits for Giardia lamblia and Cryptosporodium spp?

<p>Fixative containing iodine (B)</p> Signup and view all the answers

Why is MIF suitable for field surveys?

<p>It preserves organisms well (A)</p> Signup and view all the answers

What issue arises when using a fixative that interferes with PCR after an extended fixation time?

<p>Interference with immunoassay kits (D)</p> Signup and view all the answers

Why is iodine not suitable for some permanent stained smears such as trichrome?

<p>Iodine may cause distortion of protozoa (C)</p> Signup and view all the answers

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