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(Weber et al., 1992) Modified Ritchie’s Concentration Method Procedure Quiz

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(Weber et al., 1992) Modified Ritchie’s Concentration Method Procedure Quiz

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Questions and Answers

Which staining method is suitable for acid fast stain, safranin, and chromotrope stain?

Merthiolateiodine-formaldehyde solution

Fixative containing iodine

MIF

PVA (correct)

Why is PVA less suitable for concentration procedures?

Preserved samples do not remain stable for long

It interferes with other stains

It contains mercuric chloride

It is difficult to prepare in the laboratory (correct)

Which fixative can interfere with polymerase chain reaction (PCR) if used for an extended fixation time?

Merthiolateiodine-formaldehyde solution

Fixative containing iodine (correct)

MIF

PVA

What does iodine cause in relation to protozoa?

Distortion Signup and view all the answers

Which staining method interferes with other stains and fluorescence?

Fixative containing iodine Signup and view all the answers

Why is PVA considered good for the preservation of protozoan trophozoites and cysts?

It is easy to prepare Signup and view all the answers

Which fixative is compatible with immunoassay kits for Giardia lamblia and Cryptosporodium spp?

Fixative containing iodine Signup and view all the answers

Why is MIF suitable for field surveys?

It preserves organisms well Signup and view all the answers

What issue arises when using a fixative that interferes with PCR after an extended fixation time?

Interference with immunoassay kits Signup and view all the answers

Why is iodine not suitable for some permanent stained smears such as trichrome?

Iodine may cause distortion of protozoa Signup and view all the answers