Wave Bioreactors in Plant Cell Cultivation

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Questions and Answers

What is a potential advantage of wave bioreactors compared to stirred-tank bioreactors?

  • Higher mass transfer
  • Longer cleaning and sterilization processes
  • Reduced foaming (correct)
  • Increased shear stress

What did Eibl and Eibl achieve in terms of plant cell biomass productivity in wave bioreactors?

  • 60 g/(L.day) with a doubling time of 1 day
  • 20 g/(L.day) with a doubling time of 4 days
  • 30 g/(L.day) with a doubling time of 3 days
  • 40 g/(L.day) with a doubling time of 2 days (correct)

What is the main function of specialized membranes in membrane bioreactors?

  • In situ aeration (correct)
  • Sterilization
  • Foam reduction
  • Cell morphology observation

What was the result of using a membrane bioreactor for the production of recombinant human alpha-1-antitrypsin (AAT)?

<p>Extracellular product titre close to 250 mg/L (D)</p> Signup and view all the answers

What is an important consideration for relatively slow growing plant cells in membrane bioreactors?

<p>Mass transfer (B)</p> Signup and view all the answers

In tissue culture, which type of culture method involves the carbon source being present at the start of the culture?

<p>Batch culture (D)</p> Signup and view all the answers

What is a major limitation of 2D tissue culture systems such as T-flasks and well-plates?

<p>Non-homogeneous nature (B)</p> Signup and view all the answers

Which type of culture system exhibits concentration gradients of pH, nutrients, toxins, gases and growth factors due to lack of mixing?

<p>Static culture (C)</p> Signup and view all the answers

What is the main challenge in tissue engineering according to the text?

<p>4D challenge due to dynamic tissue growth (D)</p> Signup and view all the answers

Which type of bioreactor is currently used for primary liver cell culture and clinical liver support?

<p>Hollow fiber bioreactor (D)</p> Signup and view all the answers

What is the specific bioreactor developed for clinical liver support that accommodated 400–800 g of primary cells?

<p>Hollow fiber bioreactor (B)</p> Signup and view all the answers

What do biaxial rotating bioreactors with fluidics through in-silico modeling, as reported by Zhang et al. (2009), apply to generate?

<p>Osteogenic bone grafts (B)</p> Signup and view all the answers

What is the main result of using a spontaneous reassembly of primary cells inoculated into a bioreactor established of a scaffold or biomatrix?

<p>Establishment of well-defined liver structures (C)</p> Signup and view all the answers

What does a homogeneous mix of adult liver cells from organ collagenase digestion contain?

<p>Parenchymal hepatocytes, non-parenchymal cells, and liver progenitor cells (D)</p> Signup and view all the answers

What is the main function of bioreactors in tissue engineering according to the text?

<p>Enabling efficient exchange of nutrients and mechanical stimulus necessary for tissue engineered grafts (C)</p> Signup and view all the answers

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