Podcast
Questions and Answers
What role does Neuraminidase (NA) play in the influenza virus lifecycle?
What role does Neuraminidase (NA) play in the influenza virus lifecycle?
- It helps the virus penetrate mucus and aids in viral shedding. (correct)
- It binds the virus to host cells.
- It produces antibodies against the virus.
- It combines the genomes of different viruses.
Antigenic shift occurs solely due to point mutations in the virus's genes.
Antigenic shift occurs solely due to point mutations in the virus's genes.
False (B)
What are the two proteins that are responsible for antigenic variation in the influenza virus?
What are the two proteins that are responsible for antigenic variation in the influenza virus?
Hemagglutinin and Neuraminidase
Antigenic _____ refers to the reassortment of viral genes from two different viruses that infect the same cell.
Antigenic _____ refers to the reassortment of viral genes from two different viruses that infect the same cell.
Match the type of antigenic variation with its description:
Match the type of antigenic variation with its description:
What is a common characteristic of viruses?
What is a common characteristic of viruses?
Viruses can enter host cells without binding to any receptors.
Viruses can enter host cells without binding to any receptors.
Name one example of a virus.
Name one example of a virus.
The viral replication cycle begins with __________ of the virus to the host cell.
The viral replication cycle begins with __________ of the virus to the host cell.
Match the following types of viral entry mechanisms with their descriptions:
Match the following types of viral entry mechanisms with their descriptions:
Which of the following statements about RNA viruses is true?
Which of the following statements about RNA viruses is true?
During the uncoating phase of the viral replication cycle, the viral genome becomes exposed to the cytoplasm.
During the uncoating phase of the viral replication cycle, the viral genome becomes exposed to the cytoplasm.
What are the two spikes required for the infection process of the influenza virus?
What are the two spikes required for the infection process of the influenza virus?
What is the primary purpose of enrichment media?
What is the primary purpose of enrichment media?
All species within the Enterobacteriaceae family live in the gastrointestinal tract.
All species within the Enterobacteriaceae family live in the gastrointestinal tract.
Name one enzyme produced by bacteria that can digest lipids.
Name one enzyme produced by bacteria that can digest lipids.
The family Enterobacteriaceae belongs to the domain __________.
The family Enterobacteriaceae belongs to the domain __________.
Match the types of haemolytic bacteria with their descriptions:
Match the types of haemolytic bacteria with their descriptions:
Which of the following genera is NOT part of the Enterobacteriaceae family?
Which of the following genera is NOT part of the Enterobacteriaceae family?
MacConkey Agar is used exclusively for the growth of Gram-positive bacteria.
MacConkey Agar is used exclusively for the growth of Gram-positive bacteria.
What color do rapid fermenters produce on Eosin Methylene Blue Agar?
What color do rapid fermenters produce on Eosin Methylene Blue Agar?
The genus _____ is known for species like E. coli and E. albertii.
The genus _____ is known for species like E. coli and E. albertii.
Match the following Enterobacteriaceae genera with their species:
Match the following Enterobacteriaceae genera with their species:
What color do lactose fermenters produce on MacConkey agar?
What color do lactose fermenters produce on MacConkey agar?
Mannitol Salt Agar is selective for gram-negative bacteria.
Mannitol Salt Agar is selective for gram-negative bacteria.
What is the purpose of adding blood to Chocolate Agar?
What is the purpose of adding blood to Chocolate Agar?
Blood Agar can differentiate between ___________ bacteria.
Blood Agar can differentiate between ___________ bacteria.
Match the following enrichment media with their characteristics:
Match the following enrichment media with their characteristics:
Which type of bacteria can produce black colonies on Hektoen Enteric Agar?
Which type of bacteria can produce black colonies on Hektoen Enteric Agar?
Alpha hemolytic bacteria completely lyse red blood cells.
Alpha hemolytic bacteria completely lyse red blood cells.
What distinguishes pathogenic Staphylococci from non-pathogenic ones on Mannitol Salt Agar?
What distinguishes pathogenic Staphylococci from non-pathogenic ones on Mannitol Salt Agar?
Flashcards
Influenza Virus
Influenza Virus
A virus that causes the flu, with surface proteins (hemagglutinin and neuraminidase) that facilitate entry and release.
Hemagglutinin (HA)
Hemagglutinin (HA)
A viral surface protein that binds to sialic acid receptors on host cells, helping the virus enter.
Neuraminidase (NA)
Neuraminidase (NA)
A viral surface enzyme that helps the virus escape host cells and spread.
Antigenic Drift
Antigenic Drift
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Antigenic Shift
Antigenic Shift
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Sialic Acid
Sialic Acid
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Antigenic Variation
Antigenic Variation
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Mutations
Mutations
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Pandemic
Pandemic
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Virus
Virus
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Viral Structure
Viral Structure
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Viral Replication Cycle
Viral Replication Cycle
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Viral Attachment
Viral Attachment
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Viral Penetration
Viral Penetration
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Viral Uncoating
Viral Uncoating
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Viral Synthesis
Viral Synthesis
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Viral Assembly
Viral Assembly
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Viral Release
Viral Release
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Viral Shedding Mechanisms
Viral Shedding Mechanisms
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RNA Virus
RNA Virus
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Enrichment media
Enrichment media
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Bacterial Enzymes - Catalase
Bacterial Enzymes - Catalase
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Bacterial Enzymes - Amylase
Bacterial Enzymes - Amylase
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Bacterial Enzymes - Peptidase
Bacterial Enzymes - Peptidase
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Enterobacteriaceae Family
Enterobacteriaceae Family
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Enterobacteriaceae Genus
Enterobacteriaceae Genus
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Bacterial Colonies - Colors
Bacterial Colonies - Colors
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Media alteration
Media alteration
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Enterobacteriaceae Family
Enterobacteriaceae Family
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Eosin Methylene Blue Agar
Eosin Methylene Blue Agar
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MacConkey Agar
MacConkey Agar
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Hektoen Enteric Agar
Hektoen Enteric Agar
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Differential Media
Differential Media
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Common Human Pathogenic Enterobacteriaceae
Common Human Pathogenic Enterobacteriaceae
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Citrobacter freundii
Citrobacter freundii
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Enterobacter cloacae
Enterobacter cloacae
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Escherichia coli
Escherichia coli
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Salmonella enterica
Salmonella enterica
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Differential Media
Differential Media
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Selective Media
Selective Media
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Mannitol Salt Agar
Mannitol Salt Agar
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Lactose Fermentation
Lactose Fermentation
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Hektoen Enteric Agar
Hektoen Enteric Agar
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Bismuth Sulfite Agar
Bismuth Sulfite Agar
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Enrichment Media
Enrichment Media
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Tryptic Soy Agar/Broth
Tryptic Soy Agar/Broth
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Blood Agar
Blood Agar
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Chocolate Agar
Chocolate Agar
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Alpha hemolysis
Alpha hemolysis
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Beta hemolysis
Beta hemolysis
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Gamma hemolysis
Gamma hemolysis
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Study Notes
University of Doha for Science & Technology
- University of Doha for Science & Technology (UDST)
- College of General Education
Viruses
- Viruses are acellular infectious agents
- They cannot self-replicate
- They must infect a cell to make copies of themselves
- Examples: Influenza, HIV, Ebola
Viral Structure
- Common Features:
- RNA or DNA genome
- Capsid of proteins
- Envelope lipid bi-layer
- Surface receptors - Spikes of protein or glycoprotein
Viral Replication Cycle
- Replicative cycle depends on the virus's genome
- ssRNA viruses have a different replicative cycle than dsDNA viruses
- The image shows a general mechanism
- Attachment: Viral proteins on the envelope or capsid bind to host cell receptors
- Penetration: Entry is through one of three mechanisms: direct penetration, fusion, or receptor-mediated endocytosis
- Uncoating: The virus removes its capsid; the genome is exposed to the cytoplasm
- Transport to Nucleus: Viral genome enters the nucleus
- Synthesis (Transcription & Translation): Viral genome reproduction (transcription in nucleus) and viral protein synthesis (translation in cytoplasm)
- Assembly: New virus components are assembled to create new viruses
- Release: The virus is released from the host cell via apoptosis, budding, or exocytosis
Viral Shedding
- Three mechanisms:
- Apoptosis: Host cell lyses and releases mature viral particles.
- Budding: Virus exits through nuclear or plasma membrane, creating an envelope.
- Exocytosis: Virus leaves the host cell using vesicles.
Influenza Virus
- Enveloped RNA virus
- RNA viruses make more mistakes than DNA viruses
- They don't have self-regulation
- They adapt readily to environmental changes due to high mutation rates
- Envelope is covered with 2 proteins (antigens) required for infection: Hemagglutinin (H) spike and Neuraminidase (N) spike
Hemagglutinin (HA)
- Glycoprotein on the surface of influenza virus
- Binds to sialic acid receptors on host cells
- Helps the virus enter cells in upper respiratory tracts
- The structure of HA is affected by mutations, responsible for the emergence of new strains of influenza.
Neuraminidase (NA)
- Enzyme that helps the virus penetrate mucus
- Aids in viral shedding (budding) by cutting sialic acid from host glycoproteins.
Antigenic Variation
- Occurs in two ways for influenza viruses:
- Antigenic drift: Point mutations in genes coding for Hemagglutinin (HA) and Neuraminidase (NA)
- Antigenic shift: Reassortment of viral genes resulting in the emergence of new virus strains
Antigenic Shift
- Results from gene re-assortment of two different viruses
- Viruses exchange a large part of their genome
- The emerging virus is antigenically different from the parent viruses
- Antibodies from parent viruses are ineffective against the new virus
- Examples include H1N1 (swine flu), H5N1 (bird flu)
Swine Flu Viroid
- Re-assortment of different genetic material within a cell results in the creation of a new strain
- Genetic material from different sources re-combines
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