Week 6 & 7

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson
Download our mobile app to listen on the go
Get App

Questions and Answers

What role does Neuraminidase (NA) play in the influenza virus lifecycle?

  • It helps the virus penetrate mucus and aids in viral shedding. (correct)
  • It binds the virus to host cells.
  • It produces antibodies against the virus.
  • It combines the genomes of different viruses.

Antigenic shift occurs solely due to point mutations in the virus's genes.

False (B)

What are the two proteins that are responsible for antigenic variation in the influenza virus?

Hemagglutinin and Neuraminidase

Antigenic _____ refers to the reassortment of viral genes from two different viruses that infect the same cell.

<p>shift</p> Signup and view all the answers

Match the type of antigenic variation with its description:

<p>Antigenic Drift = Results from point mutations in viral genes Antigenic Shift = Caused by reassortment of viral genes from different viruses</p> Signup and view all the answers

What is a common characteristic of viruses?

<p>They are acellular infectious agents. (A)</p> Signup and view all the answers

Viruses can enter host cells without binding to any receptors.

<p>False (B)</p> Signup and view all the answers

Name one example of a virus.

<p>Influenza</p> Signup and view all the answers

The viral replication cycle begins with __________ of the virus to the host cell.

<p>attachment</p> Signup and view all the answers

Match the following types of viral entry mechanisms with their descriptions:

<p>Direct penetration = Only viral genome enters the host cell. Fusion = Viral envelope fuses with host membrane. Receptor mediated endocytosis = Entire virus enters via endocytosis. Exocytosis = Viruses leave using vesicles without killing the host.</p> Signup and view all the answers

Which of the following statements about RNA viruses is true?

<p>They adapt readily to environmental changes. (A)</p> Signup and view all the answers

During the uncoating phase of the viral replication cycle, the viral genome becomes exposed to the cytoplasm.

<p>True (A)</p> Signup and view all the answers

What are the two spikes required for the infection process of the influenza virus?

<p>Hemagglutinin and Neuraminidase</p> Signup and view all the answers

What is the primary purpose of enrichment media?

<p>To promote the growth of specific organisms present in small numbers (D)</p> Signup and view all the answers

All species within the Enterobacteriaceae family live in the gastrointestinal tract.

<p>False (B)</p> Signup and view all the answers

Name one enzyme produced by bacteria that can digest lipids.

<p>Lipase</p> Signup and view all the answers

The family Enterobacteriaceae belongs to the domain __________.

<p>Bacteria</p> Signup and view all the answers

Match the types of haemolytic bacteria with their descriptions:

<p>Alpha haemolytic = Partial lysis of red blood cells Beta haemolytic = Complete lysis of red blood cells Gamma haemolytic = No lysis of red blood cells Non-haemolytic = Does not produce any effects on red blood cells</p> Signup and view all the answers

Which of the following genera is NOT part of the Enterobacteriaceae family?

<p>Streptococcus (D)</p> Signup and view all the answers

MacConkey Agar is used exclusively for the growth of Gram-positive bacteria.

<p>False (B)</p> Signup and view all the answers

What color do rapid fermenters produce on Eosin Methylene Blue Agar?

<p>Green metallic colonies</p> Signup and view all the answers

The genus _____ is known for species like E. coli and E. albertii.

<p>Escherichia</p> Signup and view all the answers

Match the following Enterobacteriaceae genera with their species:

<p>Salmonella = enterica Klebsiella = pneumoniae Shigella = flexneri Yersinia = pestis</p> Signup and view all the answers

What color do lactose fermenters produce on MacConkey agar?

<p>Red (B)</p> Signup and view all the answers

Mannitol Salt Agar is selective for gram-negative bacteria.

<p>False (B)</p> Signup and view all the answers

What is the purpose of adding blood to Chocolate Agar?

<p>To provide nutrients and allow differentiation of bacteria based on their hemolytic properties.</p> Signup and view all the answers

Blood Agar can differentiate between ___________ bacteria.

<p>hemolytic</p> Signup and view all the answers

Match the following enrichment media with their characteristics:

<p>Tryptic Soy Agar = General purpose non-selective media Blood Agar = Contains 5% bovine or sheep blood Chocolate Agar = Heated blood in tryptic soy agar Broth = Liquid media for growing bacteria</p> Signup and view all the answers

Which type of bacteria can produce black colonies on Hektoen Enteric Agar?

<p>Salmonella (A)</p> Signup and view all the answers

Alpha hemolytic bacteria completely lyse red blood cells.

<p>False (B)</p> Signup and view all the answers

What distinguishes pathogenic Staphylococci from non-pathogenic ones on Mannitol Salt Agar?

<p>Pathogenic Staphylococci ferment mannitol, while non-pathogenic do not.</p> Signup and view all the answers

Flashcards

Influenza Virus

A virus that causes the flu, with surface proteins (hemagglutinin and neuraminidase) that facilitate entry and release.

Hemagglutinin (HA)

A viral surface protein that binds to sialic acid receptors on host cells, helping the virus enter.

Neuraminidase (NA)

A viral surface enzyme that helps the virus escape host cells and spread.

Antigenic Drift

Flu virus evolution through small genetic mutations in HA and NA changing antigens, creating new strains.

Signup and view all the flashcards

Antigenic Shift

Flu virus evolution through genetic reassortment of genes from two different viruses in the same host.

Signup and view all the flashcards

Sialic Acid

A receptor on vertebrate cell surfaces involved in the attachment of the influenza virus (influenza virus uses sialic acid on the host cells in the upper respiratory tract or erythrocytes).

Signup and view all the flashcards

Antigenic Variation

The process that creates new strains of influenza viruses by mutations (drift or reassortment).

Signup and view all the flashcards

Mutations

Changes in the genetic code (DNA or RNA) of the influenza virus.

Signup and view all the flashcards

Pandemic

A global outbreak of a contagious disease.

Signup and view all the flashcards

Virus

An acellular infectious agent incapable of self-replication, requiring host cell infection to reproduce.

Signup and view all the flashcards

Viral Structure

Consists of a genome (DNA or RNA), a protein capsid, and sometimes a lipid envelope with surface receptors.

Signup and view all the flashcards

Viral Replication Cycle

A series of steps leading to viral reproduction inside a host cell, varying based on the virus's genome.

Signup and view all the flashcards

Viral Attachment

Virus binds to host cell receptors with proteins on its capsid or envelope.

Signup and view all the flashcards

Viral Penetration

Entry into host cell through direct penetration, fusion, or receptor-mediated endocytosis.

Signup and view all the flashcards

Viral Uncoating

Removal of the viral capsid to expose the viral genome inside the host cell.

Signup and view all the flashcards

Viral Synthesis

Production of viral genomes and proteins.

Signup and view all the flashcards

Viral Assembly

Joining of viral genomes and capsids to form new viruses.

Signup and view all the flashcards

Viral Release

Ejection of newly assembled viruses from the host cell.

Signup and view all the flashcards

Viral Shedding Mechanisms

Apoptosis (host cell death), Budding (envelope creation), or Exocytosis (vesicles).

Signup and view all the flashcards

RNA Virus

Viruses with RNA as their genetic material.

Signup and view all the flashcards

Enrichment media

Media promoting growth of specific organisms present in small numbers, outcompeted by others.

Signup and view all the flashcards

Bacterial Enzymes - Catalase

Enzyme that breaks down hydrogen peroxide.

Signup and view all the flashcards

Bacterial Enzymes - Amylase

Enzyme breaking down long-chain carbohydrates.

Signup and view all the flashcards

Bacterial Enzymes - Peptidase

Enzyme breaking down peptides.

Signup and view all the flashcards

Enterobacteriaceae Family

Family of bacteria, mostly facultative anaerobes, ferment glucose, and typically have peritrichous flagella (except Shigella and Klebsiella).

Signup and view all the flashcards

Enterobacteriaceae Genus

Genus in the Enterobacteriaceae bacteria family

Signup and view all the flashcards

Bacterial Colonies - Colors

Bacteria can produce different colored colonies.

Signup and view all the flashcards

Media alteration

Byproducts of bacterial metabolism can change the media's appearance or characteristics.

Signup and view all the flashcards

Enterobacteriaceae Family

A family of bacteria, often causing disease in humans, that ferment glucose and typically have peritrichous flagella (except for Shigella and Klebsiella).

Signup and view all the flashcards

Eosin Methylene Blue Agar

A differential medium used to detect fecal coliform bacteria. It has pH indicators changing color based on fermentation, and inhibits Gram-positive bacteria.

Signup and view all the flashcards

MacConkey Agar

A selective and differential medium that allows for the growth of gram-negative bacteria, using lactose as a fermentable carbohydrate.

Signup and view all the flashcards

Hektoen Enteric Agar

A differential medium used to distinguish between different Enterobacteriaceae bacteria, based on lactose fermentation and the production of specific byproducts.

Signup and view all the flashcards

Differential Media

A medium that helps separate bacteria based on their metabolic properties.

Signup and view all the flashcards

Common Human Pathogenic Enterobacteriaceae

Certain genera and species of Enterobacteriaceae causing illnesses.

Signup and view all the flashcards

Citrobacter freundii

A species of bacteria in the Enterobacteriaceae family.

Signup and view all the flashcards

Enterobacter cloacae

A species of bacteria in the Enterobacteriaceae family.

Signup and view all the flashcards

Escherichia coli

A species of bacteria in the Enterobacteriaceae family, important for human health.

Signup and view all the flashcards

Salmonella enterica

A species of bacteria in the Enterobacteriaceae family, frequently causing food poisoning.

Signup and view all the flashcards

Differential Media

Media that distinguishes bacteria based on their characteristics, such as metabolism or enzyme production.

Signup and view all the flashcards

Selective Media

Media that promotes the growth of specific types of bacteria while inhibiting the growth of others.

Signup and view all the flashcards

Mannitol Salt Agar

Selective and differential media used to isolate Staphylococcus species, with high salt concentration to inhibit halophile growth.

Signup and view all the flashcards

Lactose Fermentation

The ability of bacteria to metabolize lactose.

Signup and view all the flashcards

Hektoen Enteric Agar

Selective and differential media used to isolate Salmonella and Shigella from fecal samples, using bile salts and to inhibit gram positives.

Signup and view all the flashcards

Bismuth Sulfite Agar

Selective media used to isolate Salmonella typhi, an indicator of typhoid fever, preventing gram positives and coliforms from growing, detects S.typhi with specific color.

Signup and view all the flashcards

Enrichment Media

Media that supports the growth of specific organisms in samples, allowing less numerous types to thrive.

Signup and view all the flashcards

Tryptic Soy Agar/Broth

Nonselective media that support a wide range of bacterial growth due to its nutrient richness.

Signup and view all the flashcards

Blood Agar

Enrichment media with added blood for differentiating bacteria based on their hemolytic properties.

Signup and view all the flashcards

Chocolate Agar

Blood agar with heated blood to improve its nutrient and gas exchange for bacteria.

Signup and view all the flashcards

Alpha hemolysis

Partial breakdown of red blood cells, producing a greenish discoloration of the agar.

Signup and view all the flashcards

Beta hemolysis

Complete breakdown of red blood cells, causing a clearing around the bacterial colonies.

Signup and view all the flashcards

Gamma hemolysis

No breakdown of red blood cells.

Signup and view all the flashcards

Study Notes

University of Doha for Science & Technology

  • University of Doha for Science & Technology (UDST)
  • College of General Education

Viruses

  • Viruses are acellular infectious agents
  • They cannot self-replicate
  • They must infect a cell to make copies of themselves
  • Examples: Influenza, HIV, Ebola

Viral Structure

  • Common Features:
    • RNA or DNA genome
    • Capsid of proteins
    • Envelope lipid bi-layer
    • Surface receptors - Spikes of protein or glycoprotein

Viral Replication Cycle

  • Replicative cycle depends on the virus's genome
  • ssRNA viruses have a different replicative cycle than dsDNA viruses
  • The image shows a general mechanism
    • Attachment: Viral proteins on the envelope or capsid bind to host cell receptors
    • Penetration: Entry is through one of three mechanisms: direct penetration, fusion, or receptor-mediated endocytosis
    • Uncoating: The virus removes its capsid; the genome is exposed to the cytoplasm
    • Transport to Nucleus: Viral genome enters the nucleus
    • Synthesis (Transcription & Translation): Viral genome reproduction (transcription in nucleus) and viral protein synthesis (translation in cytoplasm)
    • Assembly: New virus components are assembled to create new viruses
    • Release: The virus is released from the host cell via apoptosis, budding, or exocytosis

Viral Shedding

  • Three mechanisms:
    • Apoptosis: Host cell lyses and releases mature viral particles.
    • Budding: Virus exits through nuclear or plasma membrane, creating an envelope.
    • Exocytosis: Virus leaves the host cell using vesicles.

Influenza Virus

  • Enveloped RNA virus
  • RNA viruses make more mistakes than DNA viruses
  • They don't have self-regulation
  • They adapt readily to environmental changes due to high mutation rates
  • Envelope is covered with 2 proteins (antigens) required for infection: Hemagglutinin (H) spike and Neuraminidase (N) spike

Hemagglutinin (HA)

  • Glycoprotein on the surface of influenza virus
  • Binds to sialic acid receptors on host cells
  • Helps the virus enter cells in upper respiratory tracts
  • The structure of HA is affected by mutations, responsible for the emergence of new strains of influenza.

Neuraminidase (NA)

  • Enzyme that helps the virus penetrate mucus
  • Aids in viral shedding (budding) by cutting sialic acid from host glycoproteins.

Antigenic Variation

  • Occurs in two ways for influenza viruses:
    • Antigenic drift: Point mutations in genes coding for Hemagglutinin (HA) and Neuraminidase (NA)
    • Antigenic shift: Reassortment of viral genes resulting in the emergence of new virus strains

Antigenic Shift

  • Results from gene re-assortment of two different viruses
  • Viruses exchange a large part of their genome
  • The emerging virus is antigenically different from the parent viruses
  • Antibodies from parent viruses are ineffective against the new virus
  • Examples include H1N1 (swine flu), H5N1 (bird flu)

Swine Flu Viroid

  • Re-assortment of different genetic material within a cell results in the creation of a new strain
  • Genetic material from different sources re-combines

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Quiz Team

Related Documents

Week 6 Viruses 2023 PDF

More Like This

Vírus: Estrutura e Reprodução
15 questions
Unit 6 Viruses
16 questions

Unit 6 Viruses

RightKeytar avatar
RightKeytar
Unit 6: Viruses
16 questions

Unit 6: Viruses

RightKeytar avatar
RightKeytar
Medical Important Viruses Quiz
48 questions

Medical Important Viruses Quiz

RetractableNephrite6474 avatar
RetractableNephrite6474
Use Quizgecko on...
Browser
Browser