Virus Quantification P1: NOT ON FINAL

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Questions and Answers

What is the primary purpose of virus quantification?

  • To determine the shape and structure of viruses
  • To count the number of viruses in a specific volume and determine virus concentration (correct)
  • To identify the genetic sequence of viruses
  • To observe virus interactions with host immune cells

The virus titer is defined as:

  • The number of viruses that do not infect cells
  • The lowest concentration of viruses that still infects cells (correct)
  • The genetic material concentration of a virus
  • The number of virus particles that bind to host cells

Which of the following statements correctly differentiates biological and physical viral quantification tests?

  • Biological tests depend on viral replication, while physical tests do not require viral activity (correct)
  • Physical tests determine viral infectivity, while biological tests count all viral particles
  • Both tests measure virus infectivity directly
  • Biological tests count viral particles, while physical tests rely on cell cultures

Which of the following is an example of a biological viral quantification test?

<p>Plaque assay (C)</p>
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Which of the following is NOT a physical viral quantification test?

<p>Transformation assay (C)</p>
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What does a monolayer plaque assay measure?

<p>The number of virus particles capable of forming plaques per unit volume (B)</p>
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The pock assay, a biological viral quantification method, measures:

<p>Necrotic areas on the chorioallantoic membrane (CAM) of embryonated eggs (B)</p>
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What is the unit of measurement for a plaque assay?

<p>Plaque-forming units (PFU)/mL (B)</p>
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What does the tissue culture infectious dose 50 (TCIDâ‚…â‚€) measure?

<p>The viral dose that infects 50% of cell monolayers (A)</p>
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Match the assay with its correct unit of measurement

<p>Plaque assay = PFU/ml (Plaque-forming unit/ml) Pock assay = Pock forming units/ml (pfu/ml) Transformation assay = FFU/ml Quantal assay = Pock-forming units/ml</p>
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Flashcards

Primary purpose of virus quantification?

To count the number of viruses in a specific volume and determine virus concentration.

What is virus titer?

The lowest concentration of viruses that still infects cells.

Biological vs. physical viral quantification?

Biological tests depend on viral replication, while physical tests do not require viral activity.

What does a monolayer plaque assay measure?

Measures the number of virus particles capable of forming plaques per unit volume.

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What does the pock assay measure?

Measures necrotic areas on the chorioallantoic membrane (CAM) of embryonated eggs.

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Unit of measurement for a plaque assay?

Plaque-forming units (PFU)/mL.

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What does TCIDâ‚…â‚€ measure?

The viral dose that infects 50% of cell monolayers.

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