Virus Quantification P1

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Questions and Answers

What is the primary purpose of virus quantification?

  • To determine the shape and structure of viruses
  • To count the number of viruses in a specific volume and determine virus concentration (correct)
  • To identify the genetic sequence of viruses
  • To observe virus interactions with host immune cells

The virus titer is defined as:

  • The number of viruses that do not infect cells
  • The lowest concentration of viruses that still infects cells (correct)
  • The genetic material concentration of a virus
  • The number of virus particles that bind to host cells

Which of the following statements correctly differentiates biological and physical viral quantification tests?

  • Biological tests depend on viral replication, while physical tests do not require viral activity (correct)
  • Physical tests determine viral infectivity, while biological tests count all viral particles
  • Both tests measure virus infectivity directly
  • Biological tests count viral particles, while physical tests rely on cell cultures

Which of the following is an example of a biological viral quantification test?

<p>Plaque assay (C)</p> Signup and view all the answers

Which of the following is NOT a physical viral quantification test?

<p>Transformation assay (C)</p> Signup and view all the answers

What does a monolayer plaque assay measure?

<p>The number of virus particles capable of forming plaques per unit volume (B)</p> Signup and view all the answers

The pock assay, a biological viral quantification method, measures:

<p>Necrotic areas on the chorioallantoic membrane (CAM) of embryonated eggs (B)</p> Signup and view all the answers

What is the unit of measurement for a plaque assay?

<p>Plaque-forming units (PFU)/mL (B)</p> Signup and view all the answers

What does the tissue culture infectious dose 50 (TCIDâ‚…â‚€) measure?

<p>The viral dose that infects 50% of cell monolayers (A)</p> Signup and view all the answers

Match the assay with its correct unit of measurement

<p>Plaque assay = PFU/ml (Plaque-forming unit/ml) Pock assay = Pock forming units/ml (pfu/ml) Transformation assay = FFU/ml Quantal assay = Pock-forming units/ml</p> Signup and view all the answers

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