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Virus Quantification P1

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Questions and Answers

What is the primary purpose of virus quantification?

To determine the shape and structure of viruses

To count the number of viruses in a specific volume and determine virus concentration (correct)

To identify the genetic sequence of viruses

To observe virus interactions with host immune cells

The virus titer is defined as:

The number of viruses that do not infect cells

The lowest concentration of viruses that still infects cells (correct)

The genetic material concentration of a virus

The number of virus particles that bind to host cells

Which of the following statements correctly differentiates biological and physical viral quantification tests?

Biological tests depend on viral replication, while physical tests do not require viral activity (correct)

Physical tests determine viral infectivity, while biological tests count all viral particles

Both tests measure virus infectivity directly

Biological tests count viral particles, while physical tests rely on cell cultures

Which of the following is an example of a biological viral quantification test?

Plaque assay Signup and view all the answers

Which of the following is NOT a physical viral quantification test?

Transformation assay Signup and view all the answers

What does a monolayer plaque assay measure?

The number of virus particles capable of forming plaques per unit volume Signup and view all the answers

The pock assay, a biological viral quantification method, measures:

Necrotic areas on the chorioallantoic membrane (CAM) of embryonated eggs Signup and view all the answers

What is the unit of measurement for a plaque assay?

Plaque-forming units (PFU)/mL Signup and view all the answers

What does the tissue culture infectious dose 50 (TCID₅₀) measure?

The viral dose that infects 50% of cell monolayers Signup and view all the answers

Match the assay with its correct unit of measurement

Plaque assay = PFU/ml (Plaque-forming unit/ml) Pock assay = Pock forming units/ml (pfu/ml) Transformation assay = FFU/ml Quantal assay = Pock-forming units/ml Signup and view all the answers