Viral Replication and DNA Injection

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20 Questions

The bacterium does not burst open to release mature T4 particles.

False

The process of DNA injection into the bacterium can be visualized by cryoelectron microscopy in stained samples.

False

Prokaryotes do not have the capacity to take up nonviral DNA molecules from their surroundings.

False

Horizontal gene transfers occur frequently between different species of eukaryotes.

False

The bacterium bursts open to release immature T4 particles.

False

The DNA injection process can be visualized through light microscopy.

False

Prokaryotes do not have the ability to capture genetic information from their surroundings.

False

The heads of new T4 particles are found outside the bacterium.

False

Vibrio cholerae is a bacterium that causes cholera.

True

Escherichia coli is a pathogenic bacterium that can cause disease in humans.

False

Phototrophic species can generate oxygen as a by-product of harvesting light energy.

True

Lithotrophic species obtain their energy from consuming organic compounds.

False

Vibrio cholerae has a single flagellum at one end.

True

Escherichia coli has no flagella.

False

Viruses can infect cells of the same species only.

False

The infected cell is always killed by the massive proliferation of virus particles inside it.

False

Viruses can accidentally pick up fragments of DNA from the genome of one host cell and ferry them into another cell.

True

Horizontal transfers of genes between eukaryotic cells of different species are very common.

False

Viruses always insert their DNA into the cell's regular genome.

False

Massive transfers of genes from bacterial to eukaryotic genomes have not occurred in the evolution of mitochondria and chloroplasts.

False

Study Notes

Bacteriophages

  • The large dark objects inside the bacterium are the heads of new T4 particles in the course of assembly
  • The bacterium will burst open to release mature T4 particles
  • The process of DNA injection into the bacterium can be visualized by cryoelectron microscopy
  • Attachment of the virus to the bacterium initiates DNA injection
  • The virus head empties its DNA into the bacterium, which then bursts open to release the mature virus particles

Gene Transfer

  • Horizontal gene transfers occur frequently between different species of prokaryotes
  • Prokaryotes can take up nonviral DNA molecules from their surroundings and capture the genetic information
  • Viruses can replicate in one cell, emerge, and infect another cell, potentially of a different species
  • Viral DNA can persist in the host cell for many generations as a separate intracellular fragment of DNA (plasmid) or a sequence inserted into the cell's genome
  • Horizontal transfers of genes between eukaryotic cells of different species are very rare

Prokaryotes

  • Phototrophic species harvest light energy in various ways, some generating oxygen as a by-product
  • Lithotrophic species feed on inorganic nutrients, getting their carbon from CO2 and relying on chemicals like H2S, H2, Fe2+, or elemental sulfur for energy
  • Bacteria have a simple internal organization, with a flagellum that rotates as a propeller to drive the cell forward
  • Examples of bacteria include Vibrio cholerae and Escherichia coli (E. coli), which have flagella distributed over their surface

This quiz covers the process of viral replication and DNA injection into bacteria, including the assembly of new T4 particles and their release from the bacterial cell.

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