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Viral Protein Transport and Assembly

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Questions and Answers

What is the purpose of the mutation in the viral coat protein used in this experiment?

To prevent the fusion protein from incorporating into the plasma membrane

To allow export from the ER at high temperatures

To allow export from the ER at a low temperature (correct)

To inhibit the movement of the fusion protein to the Golgi apparatus

What is the direction of protein movement indicated by the red arrows?

From the Golgi apparatus to the ER

From the plasma membrane to the ER

From the ER to the cell surface (correct)

From the nucleus to the cytoplasm

At high temperatures, where does the fusion protein accumulate?

In the ER (correct)

At ER exit sites

In the Golgi apparatus

At the plasma membrane

What is the final destination of the fusion protein in this experiment?

The plasma membrane Signup and view all the answers

What is the role of the GFP fusion protein in this experiment?

To label the ER and track the movement of the viral coat protein Signup and view all the answers

Where does the fusion protein accumulate as the temperature is lowered?

At ER exit sites Signup and view all the answers

What is the purpose of fusing a protein with GFP?

To visualize and track the protein's movement throughout the cell Signup and view all the answers

What can be studied by fusing GFP to a protein that shuttles in and out of the nucleus?

Nuclear transport events Signup and view all the answers

What is the result of mutations in proteins involved in the secretory pathway?

Accumulation of the protein in the ER, Golgi apparatus, or transport vesicles Signup and view all the answers

What can be measured by fusing GFP to a plasma membrane protein?

The kinetics of its movement through the secretory pathway Signup and view all the answers

What is shown in Figures 15–28 and 15–29?

GFP-tagged proteins being tracked throughout the cell Signup and view all the answers

What is fused to GFP in the experiment described in Figure 15–29?

A viral coat protein Signup and view all the answers

What is the purpose of genetically fusing engineered split protein fragments X and Y to proteins A and B?

To create a noncovalently bound complex Signup and view all the answers

What is the result of the interaction between proteins A and B in the assay?

The split protein fragments X and Y regain native activity Signup and view all the answers

What is the main application of split green fluorescent proteins?

Analyzing protein-protein interactions Signup and view all the answers

What is the significance of the 2008 Nobel Prize in Chemistry?

It was awarded for the development of split green fluorescent proteins Signup and view all the answers

What is the purpose of using engineered split protein fragments X and Y?

To create a protein-protein interaction-dependent readout Signup and view all the answers