17 Questions
What is the purpose of the mutation in the viral coat protein used in this experiment?
To allow export from the ER at a low temperature
What is the direction of protein movement indicated by the red arrows?
From the ER to the cell surface
At high temperatures, where does the fusion protein accumulate?
In the ER
What is the final destination of the fusion protein in this experiment?
The plasma membrane
What is the role of the GFP fusion protein in this experiment?
To label the ER and track the movement of the viral coat protein
Where does the fusion protein accumulate as the temperature is lowered?
At ER exit sites
What is the purpose of fusing a protein with GFP?
To visualize and track the protein's movement throughout the cell
What can be studied by fusing GFP to a protein that shuttles in and out of the nucleus?
Nuclear transport events
What is the result of mutations in proteins involved in the secretory pathway?
Accumulation of the protein in the ER, Golgi apparatus, or transport vesicles
What can be measured by fusing GFP to a plasma membrane protein?
The kinetics of its movement through the secretory pathway
What is shown in Figures 15–28 and 15–29?
GFP-tagged proteins being tracked throughout the cell
What is fused to GFP in the experiment described in Figure 15–29?
A viral coat protein
What is the purpose of genetically fusing engineered split protein fragments X and Y to proteins A and B?
To create a noncovalently bound complex
What is the result of the interaction between proteins A and B in the assay?
The split protein fragments X and Y regain native activity
What is the main application of split green fluorescent proteins?
Analyzing protein-protein interactions
What is the significance of the 2008 Nobel Prize in Chemistry?
It was awarded for the development of split green fluorescent proteins
What is the purpose of using engineered split protein fragments X and Y?
To create a protein-protein interaction-dependent readout
This quiz assesses your understanding of how viral proteins are transported through the secretory pathway from the ER to the cell surface, where virus particles are assembled. It also tests your knowledge of protein mutations and their effects on protein export.
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