Podcast
Questions and Answers
What is the purpose of the mutation in the viral coat protein used in this experiment?
What is the purpose of the mutation in the viral coat protein used in this experiment?
- To prevent the fusion protein from incorporating into the plasma membrane
- To allow export from the ER at high temperatures
- To allow export from the ER at a low temperature (correct)
- To inhibit the movement of the fusion protein to the Golgi apparatus
What is the direction of protein movement indicated by the red arrows?
What is the direction of protein movement indicated by the red arrows?
- From the Golgi apparatus to the ER
- From the plasma membrane to the ER
- From the ER to the cell surface (correct)
- From the nucleus to the cytoplasm
At high temperatures, where does the fusion protein accumulate?
At high temperatures, where does the fusion protein accumulate?
- In the ER (correct)
- At ER exit sites
- In the Golgi apparatus
- At the plasma membrane
What is the final destination of the fusion protein in this experiment?
What is the final destination of the fusion protein in this experiment?
What is the role of the GFP fusion protein in this experiment?
What is the role of the GFP fusion protein in this experiment?
Where does the fusion protein accumulate as the temperature is lowered?
Where does the fusion protein accumulate as the temperature is lowered?
What is the purpose of fusing a protein with GFP?
What is the purpose of fusing a protein with GFP?
What can be studied by fusing GFP to a protein that shuttles in and out of the nucleus?
What can be studied by fusing GFP to a protein that shuttles in and out of the nucleus?
What is the result of mutations in proteins involved in the secretory pathway?
What is the result of mutations in proteins involved in the secretory pathway?
What can be measured by fusing GFP to a plasma membrane protein?
What can be measured by fusing GFP to a plasma membrane protein?
What is shown in Figures 15–28 and 15–29?
What is shown in Figures 15–28 and 15–29?
What is fused to GFP in the experiment described in Figure 15–29?
What is fused to GFP in the experiment described in Figure 15–29?
What is the purpose of genetically fusing engineered split protein fragments X and Y to proteins A and B?
What is the purpose of genetically fusing engineered split protein fragments X and Y to proteins A and B?
What is the result of the interaction between proteins A and B in the assay?
What is the result of the interaction between proteins A and B in the assay?
What is the main application of split green fluorescent proteins?
What is the main application of split green fluorescent proteins?
What is the significance of the 2008 Nobel Prize in Chemistry?
What is the significance of the 2008 Nobel Prize in Chemistry?
What is the purpose of using engineered split protein fragments X and Y?
What is the purpose of using engineered split protein fragments X and Y?
