Podcast
Questions and Answers
Which of the following statements best describes why viruses are not considered true living organisms?
Which of the following statements best describes why viruses are not considered true living organisms?
- Viruses cannot carry out metabolism on their own. (correct)
- Viruses possess cellular organelles.
- Viruses can replicate independently without a host.
- Viruses have a complex cellular structure.
What characteristic do viruses exhibit that is similar to living organisms?
What characteristic do viruses exhibit that is similar to living organisms?
- Possession of a cell membrane.
- Presence of cytoplasm.
- Independent metabolic functions.
- Ability to evolve by mutations. (correct)
Which feature is NOT associated with viruses?
Which feature is NOT associated with viruses?
- Lack of cell membrane and organelles.
- Dependency on host cell for replication.
- Replication using their own cellular machinery. (correct)
- Possessing a nucleic acid genome.
In what way do viruses depend on host cells?
In what way do viruses depend on host cells?
Which of the following is a true statement about viruses?
Which of the following is a true statement about viruses?
What is the highest taxon level used for many viruses?
What is the highest taxon level used for many viruses?
Which suffix is used for virus family names?
Which suffix is used for virus family names?
Which of the following is true about binomial nomenclature in relation to viruses?
Which of the following is true about binomial nomenclature in relation to viruses?
What is true about virus genus names?
What is true about virus genus names?
Which of these examples correctly follows the naming convention for viruses?
Which of these examples correctly follows the naming convention for viruses?
What determines the host range of a virus?
What determines the host range of a virus?
Which of the following hosts is protected from the influenza virus?
Which of the following hosts is protected from the influenza virus?
Which type of virus specifically infects fungi?
Which type of virus specifically infects fungi?
What can contribute to species resistance against certain viruses?
What can contribute to species resistance against certain viruses?
Which of the following statements is true regarding the infection of animals by measles virus?
Which of the following statements is true regarding the infection of animals by measles virus?
What is the typical size range of viruses?
What is the typical size range of viruses?
Which of the following describes helical viruses?
Which of the following describes helical viruses?
Which structure is typical of complex (binal) viruses?
Which structure is typical of complex (binal) viruses?
Enveloped viruses differ from helical and polyhedral viruses in that they are:
Enveloped viruses differ from helical and polyhedral viruses in that they are:
Which of the following correctly identifies a characteristic of polyhedral viruses?
Which of the following correctly identifies a characteristic of polyhedral viruses?
What is the term used to describe the combination of a virus's capsid and genome?
What is the term used to describe the combination of a virus's capsid and genome?
Which of the following statements correctly describes the role of spikes in viruses?
Which of the following statements correctly describes the role of spikes in viruses?
What distinguishes an enveloped virus from a naked virus?
What distinguishes an enveloped virus from a naked virus?
Which type of nucleic acid can be found in viral genomes?
Which type of nucleic acid can be found in viral genomes?
Which protein subunits make up the viral capsid?
Which protein subunits make up the viral capsid?
What is the purpose of a plaque assay method?
What is the purpose of a plaque assay method?
What do plaques represent in a lawn of bacteria during a plaque assay?
What do plaques represent in a lawn of bacteria during a plaque assay?
What does the term 'plaque-forming unit' (PFU) refer to?
What does the term 'plaque-forming unit' (PFU) refer to?
In a plaque assay, what is visualized in the agar?
In a plaque assay, what is visualized in the agar?
Which of the following best describes a plaque in the context of bacteriophages?
Which of the following best describes a plaque in the context of bacteriophages?
What is a requirement for viruses to grow?
What is a requirement for viruses to grow?
Which method is NOT used for culturing animal viruses?
Which method is NOT used for culturing animal viruses?
What indicates successful viral growth when using embryonated eggs for culturing?
What indicates successful viral growth when using embryonated eggs for culturing?
Which of the following practices does NOT involve the direct use of living cells?
Which of the following practices does NOT involve the direct use of living cells?
Which culturing method relies on direct observation of an embryo's response to viral infection?
Which culturing method relies on direct observation of an embryo's response to viral infection?
What characteristic is typical of most bacteria compared to obligate intracellular parasites like Rickettsias?
What characteristic is typical of most bacteria compared to obligate intracellular parasites like Rickettsias?
Which statement accurately describes Rickettsias?
Which statement accurately describes Rickettsias?
What is a significant difference between free-living bacteria and Rickettsias?
What is a significant difference between free-living bacteria and Rickettsias?
Which of the following best describes the survival requirements of most bacteria?
Which of the following best describes the survival requirements of most bacteria?
Which statement is NOT true about obligate intracellular parasites like Rickettsias?
Which statement is NOT true about obligate intracellular parasites like Rickettsias?
What is the primary role of interferon (IFN) in response to viral infection?
What is the primary role of interferon (IFN) in response to viral infection?
How does interferon (IFN) signal neighboring cells during a viral infection?
How does interferon (IFN) signal neighboring cells during a viral infection?
What action does interferon (IFN) induce specifically in virus-infected cells?
What action does interferon (IFN) induce specifically in virus-infected cells?
Which of the following statements about interferon (IFN) is correct?
Which of the following statements about interferon (IFN) is correct?
What effect does interferon (IFN) have on healthy cells during a viral response?
What effect does interferon (IFN) have on healthy cells during a viral response?
What does the term 'cytopathic effects' (CPE) refer to in the context of viral infections?
What does the term 'cytopathic effects' (CPE) refer to in the context of viral infections?
Which testing method is primarily used for the direct detection of viral genomic material?
Which testing method is primarily used for the direct detection of viral genomic material?
Which of the following is NOT a characteristic of serological tests for virus detection?
Which of the following is NOT a characteristic of serological tests for virus detection?
What is a key advantage of using cytopathic effect observations in viral testing?
What is a key advantage of using cytopathic effect observations in viral testing?
In what scenario would PCR tests be preferred over serological tests?
In what scenario would PCR tests be preferred over serological tests?
What occurs during the biosynthesis phase of the lytic cycle in bacterial viral infections?
What occurs during the biosynthesis phase of the lytic cycle in bacterial viral infections?
In the lysogenic cycle, what term describes viral DNA that is incorporated into the host genome?
In the lysogenic cycle, what term describes viral DNA that is incorporated into the host genome?
Which of the following statements about the lysogenic cycle is true?
Which of the following statements about the lysogenic cycle is true?
What is an evolutionary advantage of a virus's ability to enter either the lytic or lysogenic cycle?
What is an evolutionary advantage of a virus's ability to enter either the lytic or lysogenic cycle?
Which of the following best describes the role of integrase in the lysogenic cycle?
Which of the following best describes the role of integrase in the lysogenic cycle?
What happens to the varicella-zoster virus after a person recovers from chickenpox?
What happens to the varicella-zoster virus after a person recovers from chickenpox?
Which of the following describes how shingles differs from chickenpox?
Which of the following describes how shingles differs from chickenpox?
During latency, the varicella-zoster virus does which of the following?
During latency, the varicella-zoster virus does which of the following?
What is not a characteristic of the varicella-zoster virus during its latent phase?
What is not a characteristic of the varicella-zoster virus during its latent phase?
What triggers the reactivation of the varicella-zoster virus to cause shingles?
What triggers the reactivation of the varicella-zoster virus to cause shingles?
What characterizes the rash caused by chickenpox?
What characterizes the rash caused by chickenpox?
What occurs during the latent period of a viral infection?
What occurs during the latent period of a viral infection?
Which phase of a viral infection is characterized by the absence of complete virion particles inside the infected cell?
Which phase of a viral infection is characterized by the absence of complete virion particles inside the infected cell?
What is the primary consequence of viral release from an infected cell?
What is the primary consequence of viral release from an infected cell?
What distinguishes acute infections from latent infections?
What distinguishes acute infections from latent infections?
Which term best describes the new complete viruses produced during a viral infection?
Which term best describes the new complete viruses produced during a viral infection?
What is the primary purpose of transduction in molecular biology?
What is the primary purpose of transduction in molecular biology?
How does transduction facilitate horizontal gene transfer in bacteria?
How does transduction facilitate horizontal gene transfer in bacteria?
What occurs during the excision process in transduction?
What occurs during the excision process in transduction?
Which statement accurately describes the viral DNA after lysogeny?
Which statement accurately describes the viral DNA after lysogeny?
What is the outcome of a bacteriophage excising itself from a host chromosome?
What is the outcome of a bacteriophage excising itself from a host chromosome?
What is a characteristic feature of a transformed cell?
What is a characteristic feature of a transformed cell?
How do oncogenic viruses contribute to the onset of cancer?
How do oncogenic viruses contribute to the onset of cancer?
What percentage of all human cancers worldwide are estimated to be linked to oncogenic viruses?
What percentage of all human cancers worldwide are estimated to be linked to oncogenic viruses?
Which of the following best describes the effect of oncogenes on normal cells?
Which of the following best describes the effect of oncogenes on normal cells?
What is one manifestation of tumor-specific antigens in transformed cells?
What is one manifestation of tumor-specific antigens in transformed cells?
Flashcards
Why are viruses not considered living?
Why are viruses not considered living?
Viruses are not considered to be truly alive because they lack the essential features common to living organisms. They do not possess a cell membrane, cytoplasm, or cellular organelles, and they cannot carry out metabolism independently, relying on host cells for their essential functions.
How do viruses replicate?
How do viruses replicate?
Viruses lack their own machinery for replication and must depend on a host cell to provide the necessary enzymes and resources to reproduce. In essence, they hijack the host cell's replication process to create more viral particles.
What are some life-like characteristics of viruses?
What are some life-like characteristics of viruses?
Despite being non-living, viruses exhibit certain life-like characteristics that set them apart from inanimate matter. For instance, their nucleic acid genome allows them to evolve through mutations and they can replicate within a host cell by exploiting the cell's machinery.
What are viruses able to infect?
What are viruses able to infect?
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How do viruses evolve?
How do viruses evolve?
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Binomial Nomenclature
Binomial Nomenclature
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Taxonomy
Taxonomy
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Virus Family
Virus Family
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Virus Genus
Virus Genus
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Viral Classification
Viral Classification
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Host Range
Host Range
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Bacteriophages
Bacteriophages
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Mycophages
Mycophages
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Viral Receptors
Viral Receptors
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How Receptors Determine Host Range
How Receptors Determine Host Range
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How big are viruses?
How big are viruses?
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What determines a virus's shape?
What determines a virus's shape?
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Describe a helical virus.
Describe a helical virus.
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Describe a polyhedral virus.
Describe a polyhedral virus.
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What is an enveloped virus?
What is an enveloped virus?
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Viral Genome
Viral Genome
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Capsid
Capsid
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Capsomeres
Capsomeres
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Nucleocapsid
Nucleocapsid
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Envelope
Envelope
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What is a plaque in virology?
What is a plaque in virology?
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What is a plaque-forming unit (PFU)?
What is a plaque-forming unit (PFU)?
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What is the plaque assay method?
What is the plaque assay method?
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How do scientists study bacteriophages?
How do scientists study bacteriophages?
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What are bacteriophages?
What are bacteriophages?
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Why can't viruses grow in a host-free culture medium?
Why can't viruses grow in a host-free culture medium?
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How are animal viruses typically cultured?
How are animal viruses typically cultured?
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What is an animal cell culture?
What is an animal cell culture?
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How do you know if a virus is growing in an embryonated egg?
How do you know if a virus is growing in an embryonated egg?
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What are animal cell cultures used for?
What are animal cell cultures used for?
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What are free-living bacteria?
What are free-living bacteria?
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What are obligate intracellular parasites?
What are obligate intracellular parasites?
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What are Rickettsias?
What are Rickettsias?
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What are the main differences between free-living bacteria and obligate intracellular parasites?
What are the main differences between free-living bacteria and obligate intracellular parasites?
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Compare free-living bacteria and obligate intracellular parasites.
Compare free-living bacteria and obligate intracellular parasites.
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What is Interferon (IFN)?
What is Interferon (IFN)?
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How do viruses infect cells?
How do viruses infect cells?
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What is paracrine signaling?
What is paracrine signaling?
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How does IFN protect the body?
How does IFN protect the body?
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What is apoptosis?
What is apoptosis?
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Cytopathic Effects (CPE)
Cytopathic Effects (CPE)
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Serological Tests
Serological Tests
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PCR (Polymerase Chain Reaction)
PCR (Polymerase Chain Reaction)
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Plaque Assay
Plaque Assay
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Lytic Cycle
Lytic Cycle
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Lysogenic Cycle
Lysogenic Cycle
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Prophage
Prophage
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Lytic vs. Lysogenic Cycle
Lytic vs. Lysogenic Cycle
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Prophage Excision
Prophage Excision
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Latent Infection
Latent Infection
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Chickenpox and Shingles Connection
Chickenpox and Shingles Connection
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Shingles: Reactivation
Shingles: Reactivation
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Virus Dormancy
Virus Dormancy
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Dormancy Duration
Dormancy Duration
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Disease Manifestation Difference
Disease Manifestation Difference
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Latent Period
Latent Period
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Eclipse Period
Eclipse Period
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Viral Release
Viral Release
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Acute Infection
Acute Infection
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Transduction
Transduction
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Lysogeny
Lysogeny
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Imprecise excision
Imprecise excision
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Horizontal gene transfer
Horizontal gene transfer
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Oncogenic Viruses
Oncogenic Viruses
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Viral Oncogenes
Viral Oncogenes
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Transformed Cell
Transformed Cell
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Contact Inhibition
Contact Inhibition
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Tumor-Specific Antigens
Tumor-Specific Antigens
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Study Notes
Viral Characteristics
- Viruses are infectious agents, obligate intracellular parasites.
- They infect various organisms, including animals, plants, fungi, and bacteria. Some viruses, like bacteriophages, infect only bacteria, while others infect fungi (mycophages). Animal viruses affect only animals, and plant viruses infect only plants.
- Viruses are not considered living organisms.
- Viruses lack a cell membrane, cytoplasm, and cellular organelles.
- They cannot perform metabolism independently; they rely on host cell enzymes and metabolic processes.
- Viruses cannot replicate independently.
- Viral classification does not utilize the full taxonomic hierarchy (Kingdom, Phylum, Class, Order, Family, Genus, Species) found in other organisms.
- Viruses are typically much smaller than bacteria, generally ranging from 5 to 300 nanometers in size.
- Most bacteria are free-living, meaning they can survive and reproduce outside a host cell.
- Rickettsias are bacteria that are obligate intracellular parasites, meaning they must live inside the cells of another organism to survive.
Viral Structure
- Genome: The viral genome is the entire genetic information of a virus, and it can be either circular or linear.. It can be single-stranded or double-stranded DNA or RNA.
- Capsid: A protein shell surrounding the genome.
- Capsomeres: Protein subunits that compose the capsid.
- Nucleocapsid: The capsid plus the genome.
- Envelope: Some viruses have an outer membrane envelope.
- Naked/Nonenveloped Viruses: Do not have an envelope.
- Enveloped Viruses: The envelope originates from the host cell membrane.
- Spikes: Viral glycoproteins projecting from the envelope or capsid.
- Function in attaching the virus to receptors on host cells.
- Present in both enveloped and nonenveloped viruses.
- Example: H and N spikes on influenza viruses (e.g., H1N1, H2N2 influenza viruses).
Viral Shapes
- Helical Viruses: Nucleic acid is enclosed within a hollow, helical protein cylinder.
- Polyhedral Viruses: Nucleic acid is surrounded by a polyhedral (many-sided) protein shell, often in the form of an icosahedron.
- Enveloped Viruses: Nucleic acid is surrounded by a helical or polyhedral core and further covered by an envelope.
- Complex (Binal) Viruses: Possess a head and tail structure.
Viral Life-like Characteristics
- Viruses possess a nucleic acid genome.
- They exhibit evolution through mutations.
- Viruses replicate inside living host cells, utilizing host cell machinery.
Viral Taxonomy and Nomenclature
- Taxonomy is the study of classification; each level of classification is called a taxon.
- The binomial nomenclature system uses a two-part name (genus and species) to identify organisms.
- Examples include Escherichia coli and Bacillus subtilis.
- Binomial nomenclature is not used for naming viruses.
- For viral classification, "order" is often the highest level used.
- Virus family names end in "-viridae" (e.g., Coronaviridae, Orthomyxoviridae).
- Virus genus names end in "-virus" (e.g., Coronavirus, Influenza virus).
Host Range
- Most viruses infect specific hosts.
- Host range is determined by specific host receptors and cellular factors.
- Absence of specific viral receptors protects hosts and contributes to species resistance. For example, plants, fungi lack receptors for influenza viruses, making them resistant to this virus. Similarly, dogs lack receptors for measles virus.
Plaque Assay Method
- Used for culturing bacteriophages.
- Bacteriophages form plaques, which are circular clearings on a lawn of bacteria on the surface of agar. Each plaque is filled with virus.
- Plaque-forming unit (PFU): a measure of the number of infectious virus particles.
Culturing Viruses
- Viruses are obligate intracellular parasites, meaning they cannot grow in a host-free medium.
- Culturing Animal Viruses:
- Live animals: One method of culturing animal viruses.
- Embryonated eggs: Viruses can be cultured in embryonated eggs by injecting the virus into the egg and observing changes or death of the embryo.
- Animal cell cultures: Another method involving growing animal cells in a laboratory setting to culture viruses.
Interferon (IFN)
- Interferon (IFN) is a protein signaling molecule, a cytokine or hormone.
- Virus-infected cells release IFN, alerting neighboring cells via paracrine signaling.
- Paracrine signaling prompts healthy cells and immune cells to enter a viral protective mode.
- IFN also targets virus-infected cells, inducing apoptosis (programmed cell death).
Testing for the Presence of Virus
- Viral Infection - Cytopathic Effects (CPE): Changes in host cells due to viral infection, like cell lysis, can be observed under a microscope. Healthy cells vs CPE allows identification.
- Serological Tests: Antibody-based tests used to detect the presence of specific viral antigens or antibodies in a sample, such as serum.
- PCR (Polymerase Chain Reaction): A molecular technique to amplify and detect specific viral genetic material (DNA or RNA) in a sample. This is a sensitive method that can detect even low amounts of viral DNA or RNA.
Latent Infections
- Some viruses can undergo a latent infection.
- The virus initially causes an acute infection.
- Then it becomes dormant (remains hidden inside the cell).
- For example, Varicella-zoster virus causes chickenpox. Following recovery, the virus remains dormant in nerve cells.
- Years later, it can reactivate and cause shingles.
- Shingles affects nerve cells; chickenpox affects many areas.
Viral Replication - Lytic and Lysogenic Cycles
- Bacteriophage Lytic Cycle:
- Penetration: Only phage DNA enters the host cell, not the entire virus.
- Biosynthesis: Host cell machinery is used to manufacture viral components (DNA, capsid).
- Lysis: Host cell breaks up and dies, releasing new viruses.
- Animal Virus Lytic Cycle: (Details on animal viral lytic replication would be needed here for this section to be complete.)
- Lysogenic Cycle:
- Prophage/Provirus: Viral DNA integrates into the host cell's genome using viral enzyme integrase.
- Lysogenic cycle: Does not result in immediate host cell lysis.
- Prophage formation: Occurs in both bacterial and animal cells.
- Excision from host chromosome: Prophage can exit the host genome and enter the lytic cycle.
- Evolutionary Advantage: The ability to switch between lytic and lysogenic cycles provides an evolutionary benefit for the virus.
Transduction
- Transduction: A technique used by molecular biologists to introduce new genes into cells.
- Transduction in bacteria occurs naturally.
- Bacteriophages introduce genes from one bacterium to another, facilitating horizontal gene transfer.
- Transduction Process:
- Following viral infection and lysogeny, viral DNA is incorporated into the host chromosome.
- Later, the prophage (viral DNA) excises itself from the host chromosome.
- The excision process is often imprecise and may include excision of host genes as well.
- The new virus can now introduce new host genes to other species.
Additional Information from Provided Text
- Latent period: The interval from infection/entry to virion formation.
- Virions: New complete viruses.
- Eclipse period: No complete virion particles are present inside the infected cell.
- Viral Release: Results in an increase of extracellular virus; patients exhibit symptoms.
Transformation of Normal Cells into Tumor Cells
- Viral Oncogenes: Oncogenic viruses can cause cancer by disrupting normal cell growth control mechanisms, leading to uncontrolled cell proliferation and tumor formation.
- Oncogenes become integrated into the host cell’s DNA and transform normal cells into cancerous cells. Approximately 15% of human cancers worldwide are linked to oncogenic viruses.
- Transformed Cell Characteristics:
- Increased cell division
- Loss of contact inhibition
- Presence of tumor-specific antigen on cell surface and nucleus.
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