Viral Characteristics Quiz

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Questions and Answers

Which of the following statements best describes why viruses are not considered true living organisms?

  • Viruses cannot carry out metabolism on their own. (correct)
  • Viruses possess cellular organelles.
  • Viruses can replicate independently without a host.
  • Viruses have a complex cellular structure.

What characteristic do viruses exhibit that is similar to living organisms?

  • Possession of a cell membrane.
  • Presence of cytoplasm.
  • Independent metabolic functions.
  • Ability to evolve by mutations. (correct)

Which feature is NOT associated with viruses?

  • Lack of cell membrane and organelles.
  • Dependency on host cell for replication.
  • Replication using their own cellular machinery. (correct)
  • Possessing a nucleic acid genome.

In what way do viruses depend on host cells?

<p>For replication and metabolic processes. (B)</p> Signup and view all the answers

Which of the following is a true statement about viruses?

<p>They consist of nucleic acid surrounded by a protein coat. (D)</p> Signup and view all the answers

What is the highest taxon level used for many viruses?

<p>Order (C)</p> Signup and view all the answers

Which suffix is used for virus family names?

<p>-viridae (B)</p> Signup and view all the answers

Which of the following is true about binomial nomenclature in relation to viruses?

<p>It does not apply to viruses. (D)</p> Signup and view all the answers

What is true about virus genus names?

<p>They end in –virus. (D)</p> Signup and view all the answers

Which of these examples correctly follows the naming convention for viruses?

<p>Influenza B virus (B)</p> Signup and view all the answers

What determines the host range of a virus?

<p>Specific host receptors and cellular factors (A)</p> Signup and view all the answers

Which of the following hosts is protected from the influenza virus?

<p>Dogs (C)</p> Signup and view all the answers

Which type of virus specifically infects fungi?

<p>Mycophages (A)</p> Signup and view all the answers

What can contribute to species resistance against certain viruses?

<p>Absence of specific viral receptors (A)</p> Signup and view all the answers

Which of the following statements is true regarding the infection of animals by measles virus?

<p>Dogs do not have receptors for measles virus. (C)</p> Signup and view all the answers

What is the typical size range of viruses?

<p>5 to 300 nanometers (C)</p> Signup and view all the answers

Which of the following describes helical viruses?

<p>Nucleic acid surrounded by a hollow, helical protein cylinder (B)</p> Signup and view all the answers

Which structure is typical of complex (binal) viruses?

<p>Head and tail structure (B)</p> Signup and view all the answers

Enveloped viruses differ from helical and polyhedral viruses in that they are:

<p>Surrounded by an envelope (A)</p> Signup and view all the answers

Which of the following correctly identifies a characteristic of polyhedral viruses?

<p>Typically form an icosahedral structure (B)</p> Signup and view all the answers

What is the term used to describe the combination of a virus's capsid and genome?

<p>Nucleocapsid (B)</p> Signup and view all the answers

Which of the following statements correctly describes the role of spikes in viruses?

<p>Spikes function in attaching the virus to host cell receptors. (D)</p> Signup and view all the answers

What distinguishes an enveloped virus from a naked virus?

<p>Origin of the viral envelope from the host cell. (A)</p> Signup and view all the answers

Which type of nucleic acid can be found in viral genomes?

<p>Both single-stranded and double-stranded RNA or DNA. (C)</p> Signup and view all the answers

Which protein subunits make up the viral capsid?

<p>Capsomeres (B)</p> Signup and view all the answers

What is the purpose of a plaque assay method?

<p>To culture bacteriophages (D)</p> Signup and view all the answers

What do plaques represent in a lawn of bacteria during a plaque assay?

<p>Clearings filled with virus (A)</p> Signup and view all the answers

What does the term 'plaque-forming unit' (PFU) refer to?

<p>The measure of infectious virus particles (D)</p> Signup and view all the answers

In a plaque assay, what is visualized in the agar?

<p>Circular clearings on a lawn of bacteria (C)</p> Signup and view all the answers

Which of the following best describes a plaque in the context of bacteriophages?

<p>An empty zone indicating bacterial death (B)</p> Signup and view all the answers

What is a requirement for viruses to grow?

<p>A host cell (D)</p> Signup and view all the answers

Which method is NOT used for culturing animal viruses?

<p>Culturing in bacterial plates (D)</p> Signup and view all the answers

What indicates successful viral growth when using embryonated eggs for culturing?

<p>Death or abnormalities in the embryo (B)</p> Signup and view all the answers

Which of the following practices does NOT involve the direct use of living cells?

<p>Culturing virus in a nutrient agar plate (B)</p> Signup and view all the answers

Which culturing method relies on direct observation of an embryo's response to viral infection?

<p>Embryonated eggs (C)</p> Signup and view all the answers

What characteristic is typical of most bacteria compared to obligate intracellular parasites like Rickettsias?

<p>They can survive and reproduce outside a host cell. (C)</p> Signup and view all the answers

Which statement accurately describes Rickettsias?

<p>They must live inside the cells of another organism. (B)</p> Signup and view all the answers

What is a significant difference between free-living bacteria and Rickettsias?

<p>Free-living bacteria can reproduce in various environments, while Rickettsias cannot. (B)</p> Signup and view all the answers

Which of the following best describes the survival requirements of most bacteria?

<p>They can thrive in isolated environments without a host. (A)</p> Signup and view all the answers

Which statement is NOT true about obligate intracellular parasites like Rickettsias?

<p>They can reproduce independently. (B)</p> Signup and view all the answers

What is the primary role of interferon (IFN) in response to viral infection?

<p>To signal healthy and immune cells to enter a protective mode (B)</p> Signup and view all the answers

How does interferon (IFN) signal neighboring cells during a viral infection?

<p>Through paracrine signaling (D)</p> Signup and view all the answers

What action does interferon (IFN) induce specifically in virus-infected cells?

<p>Apoptosis (self-death) (C)</p> Signup and view all the answers

Which of the following statements about interferon (IFN) is correct?

<p>IFN can signal both healthy and immune cells. (C)</p> Signup and view all the answers

What effect does interferon (IFN) have on healthy cells during a viral response?

<p>It induces them to enter a protective mode. (C)</p> Signup and view all the answers

What does the term 'cytopathic effects' (CPE) refer to in the context of viral infections?

<p>Changes in host cells caused by viral infection (C)</p> Signup and view all the answers

Which testing method is primarily used for the direct detection of viral genomic material?

<p>Polymerase chain reaction (PCR) (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of serological tests for virus detection?

<p>They directly identify viral particles (D)</p> Signup and view all the answers

What is a key advantage of using cytopathic effect observations in viral testing?

<p>It can provide immediate results without complex instruments (B)</p> Signup and view all the answers

In what scenario would PCR tests be preferred over serological tests?

<p>To detect early-stage infections before antibodies are formed (D)</p> Signup and view all the answers

What occurs during the biosynthesis phase of the lytic cycle in bacterial viral infections?

<p>Bacterial machinery synthesizes viral components. (B)</p> Signup and view all the answers

In the lysogenic cycle, what term describes viral DNA that is incorporated into the host genome?

<p>Prophage (C)</p> Signup and view all the answers

Which of the following statements about the lysogenic cycle is true?

<p>It allows for viral persistence without immediate harm to the host. (C)</p> Signup and view all the answers

What is an evolutionary advantage of a virus's ability to enter either the lytic or lysogenic cycle?

<p>It allows viruses to remain dormant until conditions are favorable. (B)</p> Signup and view all the answers

Which of the following best describes the role of integrase in the lysogenic cycle?

<p>It integrates viral DNA into the host genome. (A)</p> Signup and view all the answers

What happens to the varicella-zoster virus after a person recovers from chickenpox?

<p>The virus becomes dormant within nerve cells. (C)</p> Signup and view all the answers

Which of the following describes how shingles differs from chickenpox?

<p>Shingles arises from dormant virus in nerve ganglia. (C)</p> Signup and view all the answers

During latency, the varicella-zoster virus does which of the following?

<p>Remains hidden and does not replicate. (C)</p> Signup and view all the answers

What is not a characteristic of the varicella-zoster virus during its latent phase?

<p>It becomes completely inactive. (D)</p> Signup and view all the answers

What triggers the reactivation of the varicella-zoster virus to cause shingles?

<p>A weakened immune system. (A)</p> Signup and view all the answers

What characterizes the rash caused by chickenpox?

<p>It consists of blisters covering the skin. (A)</p> Signup and view all the answers

What occurs during the latent period of a viral infection?

<p>Interval from infection to virion formation (C)</p> Signup and view all the answers

Which phase of a viral infection is characterized by the absence of complete virion particles inside the infected cell?

<p>Eclipse period (A)</p> Signup and view all the answers

What is the primary consequence of viral release from an infected cell?

<p>Increase in the number of extracellular viruses (A)</p> Signup and view all the answers

What distinguishes acute infections from latent infections?

<p>Acute infections typically exhibit symptoms and progress rapidly (B)</p> Signup and view all the answers

Which term best describes the new complete viruses produced during a viral infection?

<p>Virions (B)</p> Signup and view all the answers

What is the primary purpose of transduction in molecular biology?

<p>To introduce new genes into cells (D)</p> Signup and view all the answers

How does transduction facilitate horizontal gene transfer in bacteria?

<p>Through the introduction of genes by bacteriophages (D)</p> Signup and view all the answers

What occurs during the excision process in transduction?

<p>The prophage may include host genes in the new virus (A)</p> Signup and view all the answers

Which statement accurately describes the viral DNA after lysogeny?

<p>It gets incorporated into the host chromosome (B)</p> Signup and view all the answers

What is the outcome of a bacteriophage excising itself from a host chromosome?

<p>It may result in gene transfer to other species (D)</p> Signup and view all the answers

What is a characteristic feature of a transformed cell?

<p>Increased cell division (C)</p> Signup and view all the answers

How do oncogenic viruses contribute to the onset of cancer?

<p>By disrupting normal cell growth control mechanisms (C)</p> Signup and view all the answers

What percentage of all human cancers worldwide are estimated to be linked to oncogenic viruses?

<p>15% (D)</p> Signup and view all the answers

Which of the following best describes the effect of oncogenes on normal cells?

<p>They cause integration into the host cell’s DNA, leading to transformation. (C)</p> Signup and view all the answers

What is one manifestation of tumor-specific antigens in transformed cells?

<p>Presence on cell surface and nucleus (A)</p> Signup and view all the answers

Flashcards

Why are viruses not considered living?

Viruses are not considered to be truly alive because they lack the essential features common to living organisms. They do not possess a cell membrane, cytoplasm, or cellular organelles, and they cannot carry out metabolism independently, relying on host cells for their essential functions.

How do viruses replicate?

Viruses lack their own machinery for replication and must depend on a host cell to provide the necessary enzymes and resources to reproduce. In essence, they hijack the host cell's replication process to create more viral particles.

What are some life-like characteristics of viruses?

Despite being non-living, viruses exhibit certain life-like characteristics that set them apart from inanimate matter. For instance, their nucleic acid genome allows them to evolve through mutations and they can replicate within a host cell by exploiting the cell's machinery.

What are viruses able to infect?

Viruses, though unable to exist independently, are capable of infecting various organisms, including animals, plants, fungi, and bacteria. They are considered obligatory intracellular parasites due to their complete dependence on a host cell for survival and replication.

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How do viruses evolve?

Viruses can evolve over time through mutations in their genetic material, enabling them to adapt to new hosts or overcome host defenses. These changes in the virus's genetic code can result in the emergence of new strains or variants.

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Binomial Nomenclature

A two-part naming system used to classify organisms. The first part is the genus, and the second part is the species.

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Taxonomy

A system for classifying organisms based on their shared characteristics. Organisms are grouped into progressively smaller categories.

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Virus Family

The highest taxonomic category for viruses. Names end in '-viridae'.

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Virus Genus

A lower taxonomic category for viruses within a family. Names end in '-virus'.

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Viral Classification

The scientific naming convention for viruses. It does not use all levels of the taxonomic hierarchy used for other organisms.

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Host Range

The specific types of organisms that a virus can infect.

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Bacteriophages

Viruses that can only infect bacteria.

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Mycophages

Viruses that can only infect fungi.

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Viral Receptors

Special molecules on the surface of host cells that viruses use to attach and infect them.

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How Receptors Determine Host Range

If a host cell doesn't have the right receptors for a virus to attach, the virus can't infect it.

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How big are viruses?

Viruses are much smaller than bacteria, typically ranging from 5 to 300 nanometers in size.

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What determines a virus's shape?

The shape of a virus's protein coat (capsid) determines its classification. This coat protects the genetic material inside.

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Describe a helical virus.

A helical virus has a long, cylindrical protein coat that spirals around the genetic material like a spring.

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Describe a polyhedral virus.

A polyhedral virus has many sides, often looking like a 20-sided die (icosahedron). Its protein coat encloses the genetic material.

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What is an enveloped virus?

An enveloped virus has a helical or polyhedral core surrounded by an outer layer (envelope) made of lipids and proteins. This envelope helps the virus attach to host cells.

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Viral Genome

The entire genetic information of a virus, contained within a single molecule of DNA or RNA. It can be either circular or linear, and single- or double-stranded.

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Capsid

The protein shell that encloses the viral genome, protecting it from the environment and aiding in attachment to host cells.

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Capsomeres

A viral protein subunit that makes up the capsid.

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Nucleocapsid

The combination of the capsid and the viral genome.

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Envelope

A lipid-based membrane surrounding some viruses, derived from the host cell during viral replication.

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What is a plaque in virology?

A circular clearing on a lawn of bacteria formed by the growth of bacteriophages. Each plaque represents a single infectious virus particle (PFU).

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What is a plaque-forming unit (PFU)?

A unit used to measure the number of infectious virus particles in a sample. One plaque represents one PFU.

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What is the plaque assay method?

A method used to grow and quantify bacteriophages in the laboratory. The method involves infecting a lawn of bacteria with bacteriophages and observing the formation of plaques.

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How do scientists study bacteriophages?

A technique that utilizes a lawn of bacteria to visualize the spread of bacteriophages. It helps scientists determine the number of infectious virus particles and their ability to infect and kill bacteria.

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What are bacteriophages?

Bacteriophages are viruses that can only infect bacteria. They are important for studying viral infection and have potential as therapeutic agents.

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Why can't viruses grow in a host-free culture medium?

Viruses require a host cell to replicate, so they won't grow in a regular culture medium like bacteria do.

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How are animal viruses typically cultured?

Animal viruses can be grown in living animals, like mice, or in fertilized eggs where the virus can infect the developing embryo.

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What is an animal cell culture?

Animal cell cultures are a method used to grow animal viruses in a laboratory setting. They involve growing cells from an animal in a controlled environment.

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How do you know if a virus is growing in an embryonated egg?

The changes or death of the embryo in an embryonated egg can signal viral growth.

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What are animal cell cultures used for?

These are specialized cells grown outside of an animal in a controlled environment. They provide a convenient and efficient way to study viruses.

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What are free-living bacteria?

Most bacteria are free-living, meaning they can survive and reproduce outside a host cell.

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What are obligate intracellular parasites?

Rickettsias are bacteria that are obligate intracellular parasites, meaning they must live inside the cells of another organism to survive.

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What are Rickettsias?

Rickettsias are a type of bacteria that are obligate intracellular parasites, meaning they must live inside the cells of another organism to survive.

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What are the main differences between free-living bacteria and obligate intracellular parasites?

Bacteria that can survive and reproduce outside of a host cell are called free-living. Bacteria that require a host cell to survive are called obligate intracellular parasites.

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Compare free-living bacteria and obligate intracellular parasites.

Some bacteria, like Rickettsias, need to live inside a host cell to survive. They are known as obligate intracellular parasites. Other bacteria can survive and reproduce outside of a host cell and are called free-living bacteria.

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What is Interferon (IFN)?

A type of protein signaling molecule that acts as a cytokine (a hormone that regulates immune cells).

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How do viruses infect cells?

Virus-infected cells release IFN, which signals nearby cells to enter a 'viral protective mode' to prevent infection.

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What is paracrine signaling?

Paracrine signaling is a type of cellular communication where cells communicate directly with their local neighbors.

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How does IFN protect the body?

IFN can induce apoptosis in virus-infected cells, causing them to self-destruct and prevent the spread of the virus.

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What is apoptosis?

Apoptosis is a process of programmed cell death, a crucial mechanism for removing damaged or infected cells, preventing disease.

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Cytopathic Effects (CPE)

A visible change in the appearance of cells caused by viral infection.

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Serological Tests

Tests that detect antibodies or antigens related to a specific virus in blood samples.

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PCR (Polymerase Chain Reaction)

A highly sensitive technique that detects and amplifies specific DNA or RNA sequences from viruses.

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Plaque Assay

A method used to visually count infectious virus particles (bacteriophages) by observing the formation of clearings (plaques) on a bacteria lawn.

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Lytic Cycle

A viral infection that results in the destruction (lysis) of the host cell.

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Lysogenic Cycle

When a virus integrates its genetic material into the host cell's DNA.

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Prophage

The viral DNA that is integrated into the host cell's genome during the lysogenic cycle.

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Lytic vs. Lysogenic Cycle

The ability of a virus to either replicate immediately (lytic cycle) or integrate its DNA into the host cell's genome (lysogenic cycle).

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Prophage Excision

A virus can switch from the lysogenic cycle to the lytic cycle, causing the host cell to produce new viral particles and eventually burst.

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Latent Infection

A type of viral infection where the virus remains dormant (inactive) within the host cell, often for long periods of time.

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Chickenpox and Shingles Connection

The virus responsible for chickenpox can become dormant in the body after the initial infection, and later reactivate to cause shingles.

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Shingles: Reactivation

When the varicella-zoster virus, which causes chickenpox, is reactivated after being dormant, it can cause a painful rash called shingles.

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Virus Dormancy

The virus causing chickenpox doesn't completely disappear from the body after the initial infection, but goes dormant in nerve cells.

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Dormancy Duration

The varicella-zoster virus can remain dormant for years in nerve cells, not causing any symptoms, until it is reactivated.

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Disease Manifestation Difference

Although chickenpox and shingles are caused by the same virus, they present different symptoms and affect different parts of the body.

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Latent Period

The time period between when a virus enters a host cell and begins to produce new viral particles (virions).

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Eclipse Period

The stage of a viral infection where there are no complete viruses present inside the infected cell, only viral components are being produced.

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Viral Release

The release of new viral particles from the infected cell, leading to an increase of viruses outside of the cell and the onset of disease symptoms.

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Acute Infection

A viral infection that results in a rapid onset of symptoms and is often short-lived.

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Transduction

A technique used to introduce new genes into cells using viruses.

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Lysogeny

When viral DNA integrates into the host chromosome, potentially carrying new genes.

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Imprecise excision

The precise excision of viral DNA can sometimes include host genes, leading to their transfer.

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Horizontal gene transfer

The new virus carrying host genes can infect another cell, transferring the genes horizontally.

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Oncogenic Viruses

Viruses that can cause cancer by altering normal cell growth control mechanisms, leading to uncontrolled cell proliferation and tumor formation.

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Viral Oncogenes

Genes carried by oncogenic viruses that become integrated into the host cell's DNA, transforming normal cells into cancerous cells.

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Transformed Cell

A cell infected by an oncogenic virus exhibits increased cell division, loss of contact inhibition, and the presence of tumor-specific antigens on its surface and nucleus.

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Contact Inhibition

The ability of normal cells to stop dividing when they come into contact with other cells. This is lost in transformed cells, leading to uncontrolled growth.

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Tumor-Specific Antigens

Antigens unique to cancer cells that can be identified by the immune system. They are often found on the surface and in the nucleus of transformed cells.

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Study Notes

Viral Characteristics

  • Viruses are infectious agents, obligate intracellular parasites.
  • They infect various organisms, including animals, plants, fungi, and bacteria. Some viruses, like bacteriophages, infect only bacteria, while others infect fungi (mycophages). Animal viruses affect only animals, and plant viruses infect only plants.
  • Viruses are not considered living organisms.
  • Viruses lack a cell membrane, cytoplasm, and cellular organelles.
  • They cannot perform metabolism independently; they rely on host cell enzymes and metabolic processes.
  • Viruses cannot replicate independently.
  • Viral classification does not utilize the full taxonomic hierarchy (Kingdom, Phylum, Class, Order, Family, Genus, Species) found in other organisms.
  • Viruses are typically much smaller than bacteria, generally ranging from 5 to 300 nanometers in size.
  • Most bacteria are free-living, meaning they can survive and reproduce outside a host cell.
  • Rickettsias are bacteria that are obligate intracellular parasites, meaning they must live inside the cells of another organism to survive.

Viral Structure

  • Genome: The viral genome is the entire genetic information of a virus, and it can be either circular or linear.. It can be single-stranded or double-stranded DNA or RNA.
  • Capsid: A protein shell surrounding the genome.
  • Capsomeres: Protein subunits that compose the capsid.
  • Nucleocapsid: The capsid plus the genome.
  • Envelope: Some viruses have an outer membrane envelope.
    • Naked/Nonenveloped Viruses: Do not have an envelope.
    • Enveloped Viruses: The envelope originates from the host cell membrane.
  • Spikes: Viral glycoproteins projecting from the envelope or capsid.
    • Function in attaching the virus to receptors on host cells.
    • Present in both enveloped and nonenveloped viruses.
    • Example: H and N spikes on influenza viruses (e.g., H1N1, H2N2 influenza viruses).

Viral Shapes

  • Helical Viruses: Nucleic acid is enclosed within a hollow, helical protein cylinder.
  • Polyhedral Viruses: Nucleic acid is surrounded by a polyhedral (many-sided) protein shell, often in the form of an icosahedron.
  • Enveloped Viruses: Nucleic acid is surrounded by a helical or polyhedral core and further covered by an envelope.
  • Complex (Binal) Viruses: Possess a head and tail structure.

Viral Life-like Characteristics

  • Viruses possess a nucleic acid genome.
  • They exhibit evolution through mutations.
  • Viruses replicate inside living host cells, utilizing host cell machinery.

Viral Taxonomy and Nomenclature

  • Taxonomy is the study of classification; each level of classification is called a taxon.
  • The binomial nomenclature system uses a two-part name (genus and species) to identify organisms.
  • Examples include Escherichia coli and Bacillus subtilis.
  • Binomial nomenclature is not used for naming viruses.
  • For viral classification, "order" is often the highest level used.
  • Virus family names end in "-viridae" (e.g., Coronaviridae, Orthomyxoviridae).
  • Virus genus names end in "-virus" (e.g., Coronavirus, Influenza virus).

Host Range

  • Most viruses infect specific hosts.
  • Host range is determined by specific host receptors and cellular factors.
  • Absence of specific viral receptors protects hosts and contributes to species resistance. For example, plants, fungi lack receptors for influenza viruses, making them resistant to this virus. Similarly, dogs lack receptors for measles virus.

Plaque Assay Method

  • Used for culturing bacteriophages.
  • Bacteriophages form plaques, which are circular clearings on a lawn of bacteria on the surface of agar. Each plaque is filled with virus.
  • Plaque-forming unit (PFU): a measure of the number of infectious virus particles.

Culturing Viruses

  • Viruses are obligate intracellular parasites, meaning they cannot grow in a host-free medium.
  • Culturing Animal Viruses:
    • Live animals: One method of culturing animal viruses.
    • Embryonated eggs: Viruses can be cultured in embryonated eggs by injecting the virus into the egg and observing changes or death of the embryo.
    • Animal cell cultures: Another method involving growing animal cells in a laboratory setting to culture viruses.

Interferon (IFN)

  • Interferon (IFN) is a protein signaling molecule, a cytokine or hormone.
  • Virus-infected cells release IFN, alerting neighboring cells via paracrine signaling.
  • Paracrine signaling prompts healthy cells and immune cells to enter a viral protective mode.
  • IFN also targets virus-infected cells, inducing apoptosis (programmed cell death).

Testing for the Presence of Virus

  • Viral Infection - Cytopathic Effects (CPE): Changes in host cells due to viral infection, like cell lysis, can be observed under a microscope. Healthy cells vs CPE allows identification.
  • Serological Tests: Antibody-based tests used to detect the presence of specific viral antigens or antibodies in a sample, such as serum.
  • PCR (Polymerase Chain Reaction): A molecular technique to amplify and detect specific viral genetic material (DNA or RNA) in a sample. This is a sensitive method that can detect even low amounts of viral DNA or RNA.

Latent Infections

  • Some viruses can undergo a latent infection.
  • The virus initially causes an acute infection.
  • Then it becomes dormant (remains hidden inside the cell).
  • For example, Varicella-zoster virus causes chickenpox. Following recovery, the virus remains dormant in nerve cells.
  • Years later, it can reactivate and cause shingles.
  • Shingles affects nerve cells; chickenpox affects many areas.

Viral Replication - Lytic and Lysogenic Cycles

  • Bacteriophage Lytic Cycle:
    • Penetration: Only phage DNA enters the host cell, not the entire virus.
    • Biosynthesis: Host cell machinery is used to manufacture viral components (DNA, capsid).
    • Lysis: Host cell breaks up and dies, releasing new viruses.
  • Animal Virus Lytic Cycle: (Details on animal viral lytic replication would be needed here for this section to be complete.)
  • Lysogenic Cycle:
    • Prophage/Provirus: Viral DNA integrates into the host cell's genome using viral enzyme integrase.
    • Lysogenic cycle: Does not result in immediate host cell lysis.
    • Prophage formation: Occurs in both bacterial and animal cells.
    • Excision from host chromosome: Prophage can exit the host genome and enter the lytic cycle.
    • Evolutionary Advantage: The ability to switch between lytic and lysogenic cycles provides an evolutionary benefit for the virus.

Transduction

  • Transduction: A technique used by molecular biologists to introduce new genes into cells.
  • Transduction in bacteria occurs naturally.
  • Bacteriophages introduce genes from one bacterium to another, facilitating horizontal gene transfer.
  • Transduction Process:
    • Following viral infection and lysogeny, viral DNA is incorporated into the host chromosome.
    • Later, the prophage (viral DNA) excises itself from the host chromosome.
    • The excision process is often imprecise and may include excision of host genes as well.
    • The new virus can now introduce new host genes to other species.

Additional Information from Provided Text

  • Latent period: The interval from infection/entry to virion formation.
  • Virions: New complete viruses.
  • Eclipse period: No complete virion particles are present inside the infected cell.
  • Viral Release: Results in an increase of extracellular virus; patients exhibit symptoms.

Transformation of Normal Cells into Tumor Cells

  • Viral Oncogenes: Oncogenic viruses can cause cancer by disrupting normal cell growth control mechanisms, leading to uncontrolled cell proliferation and tumor formation.
  • Oncogenes become integrated into the host cell’s DNA and transform normal cells into cancerous cells. Approximately 15% of human cancers worldwide are linked to oncogenic viruses.
  • Transformed Cell Characteristics:
    • Increased cell division
    • Loss of contact inhibition
    • Presence of tumor-specific antigen on cell surface and nucleus.

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