18 Questions
Which evacuated tube and additive combination is used for coagulation studies?
Black top with buffered sodium citrate
What is the purpose of ethylene diamine tetraacetic acid (EDTA) in the purple/lavender top tube?
To remove calcium
In which evacuated tube would you find gel separator as an additive?
Gold top
Which evacuated tube should be used for glucose determination?
Brown/tan top
What does the green top evacuated tube contain as an additive?
Heparin
Which evacuated tube is specifically used for prothrombin time and APTT testing?
Light blue top
What is the purpose of using evacuated tubes in venipuncture?
To create a partial vacuum to aid in blood draw
What is the function of anticoagulant additives in evacuated tubes?
To prevent clotting of the blood sample
How is the vacuum inside evacuated tubes created?
By manually pulling air out of the tube
What does the color of the stopper on an evacuated tube indicate?
The type of anticoagulant present in the tube
Why are evacuated tubes preferred for venipuncture procedures?
To allow for more tubes to be collected simultaneously
What is the role of a rubber sheath on a two-way/multi-sample needle?
To prevent blood leakage during sample collection
Which additive is used for blood culture specimen collection?
Sodium polyanethol sulfonate (SPS)
What is the purpose of using sodium heparin in evacuated tubes?
Inactivates thrombin and thromboplastin
What type of testing is typically done using royal blue top evacuated tubes?
Toxicology testing
Which evacuated tube is suitable for routine chemistry tests?
Light green top with lithium heparin
What does the pink top evacuated tube primarily signify?
Blood banking tests
Why is it important to position the patient's arm slightly bent in a downward position during blood collection?
Prevents blood from touching the stopper puncturing needle
Study Notes
Evacuated Tubes and Additives
- Coagulation studies use a combination of sodium citrate tube and citrate additive.
- EDTA (ethylene diamine tetraacetic acid) in purple/lavender top tubes prevents blood from clotting by binding to calcium ions, making it suitable for hematology tests.
- Gel separator additive is found in serum separator tubes (SSTs).
- For glucose determination, use a grey top tube containing sodium fluoride (NaF) and potassium oxalate (K2C2O4) as additives.
- Green top tubes contain heparin as an additive, which prevents blood clotting.
- Blue top tubes containing sodium citrate are specifically used for prothrombin time and APTT testing.
Purpose and Function of Evacuated Tubes
- Evacuated tubes are used in venipuncture to reduce hemolysis and facilitate blood collection.
- Anticoagulant additives in evacuated tubes prevent blood clotting, allowing for smooth blood flow and reducing platelet activation.
- The vacuum inside evacuated tubes is created by removing air from the tube, causing blood to flow in when the tube is punctured.
- The color of the stopper on an evacuated tube indicates the type of additive or test it is designed for.
- Evacuated tubes are preferred for venipuncture procedures due to their convenience, safety, and ability to minimize hemolysis.
Additional Information
- The rubber sheath on a two-way/multi-sample needle helps to seal the needle, preventing blood from flowing out.
- Sodium polyanethol sulfonate (SPS) is used as an additive for blood culture specimen collection.
- Sodium heparin in evacuated tubes prevents blood clotting and is used for plasma-based tests.
- Royal blue top evacuated tubes are typically used for trace element testing.
- Red top tubes or serum separator tubes (SSTs) are suitable for routine chemistry tests.
- Pink top evacuated tubes primarily signify that the tube contains K2EDTA (potassium EDTA) for hematology tests.
- Positioning the patient's arm slightly bent in a downward position during blood collection helps to reduce hemolysis and facilitate blood flow.
This quiz covers the process of collecting blood specimens for diagnostic or monitoring purposes using the Evacuated Tube System method. Topics include equipment needed, safety measures, and the procedure for drawing blood from an antecubital vein.
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