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UV/Vis-Spectroscopy: Electromagnetic Radiation Fundamentals

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UV/Vis-Spectroscopy: Electromagnetic Radiation Fundamentals

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Questions and Answers

What condition must be met for absorption to occur?

The optical path length must be short

The chromophore concentration must be low

The transition dipole moment must not be equal to 0 (correct)

The transition dipole moment must be 0

How does a larger transition dipole moment affect the interaction between a molecule and light?

It weakens the interaction

It has no effect on the interaction

It changes the color of the molecule

It strengthens the interaction (correct)

What is the role of Lambert-Beer's Law in absorption?

Determining the molecular weight of a compound

Calculating the optical path length

Relating absorption to optical path length, chromophore concentration, and specific absorbance (correct)

Measuring the intensity of light absorbed by a sample

What does it mean when a sample has a red color?

It reflects all light Signup and view all the answers

Why is it important to choose the correct cuvettes for UV/Vis absorption measurements?

To ensure accurate transmission of light at specific wavelengths Signup and view all the answers

What does the Lambert-Beer law indicate about protein concentration and absorption?

Protein concentration is directly proportional to absorption Signup and view all the answers

What type of transitions involve the excitation of an electron from a bonding molecular orbital to an antibonding molecular orbital?

π→π* Transitions Signup and view all the answers

Which electromagnetic field component is relevant for optical spectroscopy?

Electric field Signup and view all the answers

What defines the resonance conditions for absorption in UV/Vis spectroscopy?

Energy difference between electronic states Signup and view all the answers

Which type of transitions involve the excitation of an electron from a non-bonding molecular orbital to a π* antibonding molecular orbital?

σ→π* Transitions Signup and view all the answers

What happens during π→π∗ transitions in UV/Vis spectroscopy?

Electron excitation from a bonding to an anti-bonding orbital Signup and view all the answers

What is the period in space for optical spectroscopy where [λ] = nm?

[λ] = nm Signup and view all the answers