Podcast
Questions and Answers
What condition must be met for absorption to occur?
What condition must be met for absorption to occur?
- The optical path length must be short
- The chromophore concentration must be low
- The transition dipole moment must not be equal to 0 (correct)
- The transition dipole moment must be 0
How does a larger transition dipole moment affect the interaction between a molecule and light?
How does a larger transition dipole moment affect the interaction between a molecule and light?
- It weakens the interaction
- It has no effect on the interaction
- It changes the color of the molecule
- It strengthens the interaction (correct)
What is the role of Lambert-Beer's Law in absorption?
What is the role of Lambert-Beer's Law in absorption?
- Determining the molecular weight of a compound
- Calculating the optical path length
- Relating absorption to optical path length, chromophore concentration, and specific absorbance (correct)
- Measuring the intensity of light absorbed by a sample
What does it mean when a sample has a red color?
What does it mean when a sample has a red color?
Why is it important to choose the correct cuvettes for UV/Vis absorption measurements?
Why is it important to choose the correct cuvettes for UV/Vis absorption measurements?
What does the Lambert-Beer law indicate about protein concentration and absorption?
What does the Lambert-Beer law indicate about protein concentration and absorption?
What type of transitions involve the excitation of an electron from a bonding molecular orbital to an antibonding molecular orbital?
What type of transitions involve the excitation of an electron from a bonding molecular orbital to an antibonding molecular orbital?
Which electromagnetic field component is relevant for optical spectroscopy?
Which electromagnetic field component is relevant for optical spectroscopy?
What defines the resonance conditions for absorption in UV/Vis spectroscopy?
What defines the resonance conditions for absorption in UV/Vis spectroscopy?
Which type of transitions involve the excitation of an electron from a non-bonding molecular orbital to a π* antibonding molecular orbital?
Which type of transitions involve the excitation of an electron from a non-bonding molecular orbital to a π* antibonding molecular orbital?
What happens during π→π∗ transitions in UV/Vis spectroscopy?
What happens during π→π∗ transitions in UV/Vis spectroscopy?
What is the period in space for optical spectroscopy where [λ] = nm?
What is the period in space for optical spectroscopy where [λ] = nm?
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