learn anything glow cytometry 3

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What is flow cytometry?

A technique for the study of a suspension of cells in a stream of gas that are detected by a chemical system.
A technique for the study of a suspension of cells in a stream of liquid that are detected by a mechanical system.
A technique for the study of a suspension of cells in a stream of plasma that are detected by a biological system.
A technique for the study of a suspension of cells in a stream of fluid that are detected by an electronic system. (correct)

What is flow cytometry?

A technique for the study of a suspension of cells in a stream of gas that are detected by a chemical system.
A technique for the study of a suspension of cells in a stream of liquid that are detected by a mechanical system.
A technique for the study of a suspension of cells in a stream of plasma that are detected by a biological system.
A technique for the study of a suspension of cells in a stream of fluid that are detected by an electronic system. (correct)

What does FC allow for?

The simultaneous uniparametric analysis of physical and biochemical characteristics of thousands of particles per second
The sequential multiparametric analysis of physical and biochemical characteristics of thousands of particles per second
The simultaneous multiparametric analysis of physical and biochemical characteristics of thousands of particles per second (correct)
The sequential uniparametric analysis of physical and biochemical characteristics of thousands of particles per second

What does FC allow for?

The simultaneous multiparametric analysis of physical and biochemical characteristics of thousands of particles per second (C) Signup and view all the answers

What is the primary use of FC?

To examine single events and is particularly useful for single cell event analysis through multiparametric analysis and immunochemical detection. (C) Signup and view all the answers

What is the primary use of FC?

To examine single events and is particularly useful for single cell event analysis through multiparametric analysis and immunochemical detection. (C) Signup and view all the answers

What is the purpose of FC analysis parameters?

To check the complexity and size of a cell. (D) Signup and view all the answers

What is the purpose of FC analysis parameters?

To check the complexity and size of a cell. (D) Signup and view all the answers

What is the principle used to avoid the problems of duplets in FC analysis?

The principle of singlet discrimination. (B) Signup and view all the answers

What is the principle used to avoid the problems of duplets in FC analysis?

The principle of singlet discrimination. (B) Signup and view all the answers

What is the specific type of plot used for the detection of several epitopes present on a cell membrane?

Dot plot. (D) Signup and view all the answers

What is the specific type of plot used for the detection of several epitopes present on a cell membrane?

Dot plot. (D) Signup and view all the answers

What is the BrdU incorporation assay used for?

Cell proliferation. (B) Signup and view all the answers

What is the BrdU incorporation assay used for?

Cell proliferation. (B) Signup and view all the answers

What are the steps involved in the BrdU incorporation assay?

Fixation, permeabilization, Dnase treatment, and antibody labelling, followed by co-staining for total DNA content using fluorescent intercalator 7-aminoactinomycin D, or 7AAD (C) Signup and view all the answers

What are the steps involved in the BrdU incorporation assay?

Fixation, permeabilization, Dnase treatment, and antibody labelling, followed by co-staining for total DNA content using fluorescent intercalator 7-aminoactinomycin D, or 7AAD (C) Signup and view all the answers