learn anything glow cytometry 3
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What is flow cytometry?

  • A technique for the study of a suspension of cells in a stream of gas that are detected by a chemical system.
  • A technique for the study of a suspension of cells in a stream of liquid that are detected by a mechanical system.
  • A technique for the study of a suspension of cells in a stream of plasma that are detected by a biological system.
  • A technique for the study of a suspension of cells in a stream of fluid that are detected by an electronic system. (correct)

    • What is flow cytometry?

  • A technique for the study of a suspension of cells in a stream of gas that are detected by a chemical system.
  • A technique for the study of a suspension of cells in a stream of liquid that are detected by a mechanical system.
  • A technique for the study of a suspension of cells in a stream of plasma that are detected by a biological system.
  • A technique for the study of a suspension of cells in a stream of fluid that are detected by an electronic system. (correct)

    • What does FC allow for?

  • The simultaneous uniparametric analysis of physical and biochemical characteristics of thousands of particles per second
  • The sequential multiparametric analysis of physical and biochemical characteristics of thousands of particles per second
  • The simultaneous multiparametric analysis of physical and biochemical characteristics of thousands of particles per second (correct)
  • The sequential uniparametric analysis of physical and biochemical characteristics of thousands of particles per second

    • What does FC allow for?

    <p>The simultaneous multiparametric analysis of physical and biochemical characteristics of thousands of particles per second</p> Signup and view all the answers

    What is the primary use of FC?

    <p>To examine single events and is particularly useful for single cell event analysis through multiparametric analysis and immunochemical detection.</p> Signup and view all the answers

    What is the primary use of FC?

    <p>To examine single events and is particularly useful for single cell event analysis through multiparametric analysis and immunochemical detection.</p> Signup and view all the answers

    What is the purpose of FC analysis parameters?

    <p>To check the complexity and size of a cell.</p> Signup and view all the answers

    What is the purpose of FC analysis parameters?

    <p>To check the complexity and size of a cell.</p> Signup and view all the answers

    What is the principle used to avoid the problems of duplets in FC analysis?

    <p>The principle of singlet discrimination.</p> Signup and view all the answers

    What is the principle used to avoid the problems of duplets in FC analysis?

    <p>The principle of singlet discrimination.</p> Signup and view all the answers

    What is the specific type of plot used for the detection of several epitopes present on a cell membrane?

    <p>Dot plot.</p> Signup and view all the answers

    What is the specific type of plot used for the detection of several epitopes present on a cell membrane?

    <p>Dot plot.</p> Signup and view all the answers

    What is the BrdU incorporation assay used for?

    <p>Cell proliferation.</p> Signup and view all the answers

    What is the BrdU incorporation assay used for?

    <p>Cell proliferation.</p> Signup and view all the answers

    What are the steps involved in the BrdU incorporation assay?

    <p>Fixation, permeabilization, Dnase treatment, and antibody labelling, followed by co-staining for total DNA content using fluorescent intercalator 7-aminoactinomycin D, or 7AAD</p> Signup and view all the answers

    What are the steps involved in the BrdU incorporation assay?

    <p>Fixation, permeabilization, Dnase treatment, and antibody labelling, followed by co-staining for total DNA content using fluorescent intercalator 7-aminoactinomycin D, or 7AAD</p> Signup and view all the answers

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