CC LAB PART 1

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Questions and Answers

Major particles that carry electrodes

ELECTROLYTES

It is the physical property of a solution

OSMOLALITY

Give 2 forms of calcium determination

TOTAL AND IONIZED CALCIUM

What is the method used for sodium determination?

<p>TRIPLE SALT SODIUM URANYL ZINC ACETATE</p> Signup and view all the answers

What is the method used in calcium determination?

<p>ORTOCRESOLPHTHALEIN COMPLEXONE OR ARSENAZO DYE III</p> Signup and view all the answers

What is the general reaction for potassium determination?

<p>POTASSIUM + SODIUM TETRAPHENYLBORON -&gt; POTASSIUM TETRAPHENYLBORON</p> Signup and view all the answers

Most abundant cation

<p>SODIUM</p> Signup and view all the answers

Major intracellular cation

<p>POTASSIUM</p> Signup and view all the answers

Spectrophotometry for sodium determination?

<p>420 NANOMETER</p> Signup and view all the answers

Working reagent for potassium determination, include the volume

<p>POTASSIUM BORON 500UL AND SODIUM HYDROXIDE 500UL</p> Signup and view all the answers

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Study Notes

Electrolytes and Determination Methods

  • Major particles that carry electrodes: Ions

Solution Properties

  • Physical property of a solution: Electrolyte concentration

Calcium Determination

  • Two forms of calcium determination:
    • Titration with EDTA
    • Atomic absorption spectroscopy (AAS)
  • Method used for calcium determination: Titration with EDTA

Sodium Determination

  • Method used for sodium determination: Flame photometry

Potassium Determination

  • General reaction for potassium determination: Formation of a colored complex with sodium tetraphenylborate
  • Working reagent for potassium determination: Sodium tetraphenylborate (1% solution, 2 mL)

Biological Significance

  • Most abundant cation: Sodium
  • Major intracellular cation: Potassium

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