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CC LAB PART 1

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10 Questions

Major particles that carry electrodes

ELECTROLYTES

It is the physical property of a solution

OSMOLALITY

Give 2 forms of calcium determination

TOTAL AND IONIZED CALCIUM

What is the method used for sodium determination?

TRIPLE SALT SODIUM URANYL ZINC ACETATE

What is the method used in calcium determination?

ORTOCRESOLPHTHALEIN COMPLEXONE OR ARSENAZO DYE III

What is the general reaction for potassium determination?

POTASSIUM + SODIUM TETRAPHENYLBORON -> POTASSIUM TETRAPHENYLBORON

Most abundant cation

SODIUM

Major intracellular cation

POTASSIUM

Spectrophotometry for sodium determination?

420 NANOMETER

Working reagent for potassium determination, include the volume

POTASSIUM BORON 500UL AND SODIUM HYDROXIDE 500UL

Study Notes

Electrolytes and Determination Methods

  • Major particles that carry electrodes: Ions

Solution Properties

  • Physical property of a solution: Electrolyte concentration

Calcium Determination

  • Two forms of calcium determination:
    • Titration with EDTA
    • Atomic absorption spectroscopy (AAS)
  • Method used for calcium determination: Titration with EDTA

Sodium Determination

  • Method used for sodium determination: Flame photometry

Potassium Determination

  • General reaction for potassium determination: Formation of a colored complex with sodium tetraphenylborate
  • Working reagent for potassium determination: Sodium tetraphenylborate (1% solution, 2 mL)

Biological Significance

  • Most abundant cation: Sodium
  • Major intracellular cation: Potassium

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