29 Questions
What is Isothermal Titration Calorimetry (ITC) used for?
Measuring the energy released or absorbed during a chemical reaction
What is Isothermal Titration Calorimetry (ITC)?
A technique used to measure the energy released or absorbed during a chemical reaction.
What is Isothermal Titration Calorimetry (ITC)?
A technique used to measure the energy released or absorbed during a chemical reaction.
What is ligand binding?
When a small molecule binds to a larger molecule
What is ligand binding?
When a small molecule binds to a larger molecule
What is ligand binding?
When a small molecule binds to a larger molecule
What fields benefit from understanding ligand binding?
Drug design and molecular biology research
Why is understanding ligand binding important?
Both A and B
Why is understanding ligand binding important?
Both A and B
How does ITC measure the energy change during ligand binding?
By measuring the heat change that occurs when a ligand is added to a receptor solution
How does ITC work?
It measures the heat change that occurs when a ligand is added to a receptor solution.
How does ITC work?
It measures the heat change that occurs when a ligand is added to a receptor solution.
What can be calculated using the heat change measured by ITC?
The energy released or absorbed during the binding process.
What can be calculated using the heat change measured by ITC?
The energy released or absorbed during the binding process.
What information can be obtained from a single ITC experiment?
Stoichiometry, association constant, and binding enthalpy
What information can ITC provide about ligand binding?
All of the above
What information can ITC provide about ligand binding?
All of the above
What is an advantage of ITC?
All of the above
Does ITC require labeling or modification of the ligand or receptor?
No, it does not require labeling.
Does ITC require labeling or modification of the ligand or receptor?
No, it does not require labeling.
What is a disadvantage of ITC for non-covalent interactions?
The signal that ITC measures (the binding enthalpy) can be relatively small
What is a disadvantage of ITC?
All of the above
What is a disadvantage of ITC?
All of the above
What is a disadvantage of ITC regarding heat change measurement?
The heat change measured by ITC includes contributions from all processes occurring in the solution
What is a disadvantage of ITC regarding speed and sample amount?
The technique can be slow and requires relatively large amounts of sample
Is ITC suitable for high-throughput screening?
No, it is not suitable
Is ITC suitable for high-throughput screening?
No, it is not suitable
Should scientists use ITC in conjunction with other methods?
Yes, it is important for scientists to use ITC in conjunction with other methods.
Should scientists use ITC in conjunction with other methods?
Yes, it is important for scientists to use ITC in conjunction with other methods.
Study Notes
Scisummary (SIMPLIFIED)
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