ITC quiz 2
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Questions and Answers

What is Isothermal Titration Calorimetry (ITC) used for?

  • Studying the process of photosynthesis
  • Studying the properties of light absorption
  • Measuring the energy released or absorbed during a chemical reaction (correct)
  • Measuring the mass of a molecule

What is Isothermal Titration Calorimetry (ITC)?

  • A technique used to measure the size of a receptor.
  • A technique used to measure the concentration of a ligand
  • A technique used to measure the pH of a solution.
  • A technique used to measure the energy released or absorbed during a chemical reaction. (correct)

What is Isothermal Titration Calorimetry (ITC)?

  • A technique used to measure the size of a receptor.
  • A technique used to measure the concentration of a ligand
  • A technique used to measure the pH of a solution.
  • A technique used to measure the energy released or absorbed during a chemical reaction. (correct)

What is ligand binding?

<p>When a small molecule binds to a larger molecule (D)</p> Signup and view all the answers

What is ligand binding?

<p>When a small molecule binds to a larger molecule (C)</p> Signup and view all the answers

What is ligand binding?

<p>When a small molecule binds to a larger molecule (D)</p> Signup and view all the answers

What fields benefit from understanding ligand binding?

<p>Drug design and molecular biology research (B)</p> Signup and view all the answers

Why is understanding ligand binding important?

<p>Both A and B (A)</p> Signup and view all the answers

Why is understanding ligand binding important?

<p>Both A and B (A)</p> Signup and view all the answers

How does ITC measure the energy change during ligand binding?

<p>By measuring the heat change that occurs when a ligand is added to a receptor solution (B)</p> Signup and view all the answers

How does ITC work?

<p>It measures the heat change that occurs when a ligand is added to a receptor solution. (D)</p> Signup and view all the answers

How does ITC work?

<p>It measures the heat change that occurs when a ligand is added to a receptor solution. (D)</p> Signup and view all the answers

What can be calculated using the heat change measured by ITC?

<p>The energy released or absorbed during the binding process. (B)</p> Signup and view all the answers

What can be calculated using the heat change measured by ITC?

<p>The energy released or absorbed during the binding process. (B)</p> Signup and view all the answers

What information can be obtained from a single ITC experiment?

<p>Stoichiometry, association constant, and binding enthalpy (C)</p> Signup and view all the answers

What information can ITC provide about ligand binding?

<p>All of the above (B)</p> Signup and view all the answers

What information can ITC provide about ligand binding?

<p>All of the above (B)</p> Signup and view all the answers

What is an advantage of ITC?

<p>All of the above (A)</p> Signup and view all the answers

Does ITC require labeling or modification of the ligand or receptor?

<p>No, it does not require labeling. (B)</p> Signup and view all the answers

Does ITC require labeling or modification of the ligand or receptor?

<p>No, it does not require labeling. (B)</p> Signup and view all the answers

What is a disadvantage of ITC for non-covalent interactions?

<p>The signal that ITC measures (the binding enthalpy) can be relatively small (D)</p> Signup and view all the answers

What is a disadvantage of ITC?

<p>All of the above (C)</p> Signup and view all the answers

What is a disadvantage of ITC?

<p>All of the above (C)</p> Signup and view all the answers

What is a disadvantage of ITC regarding heat change measurement?

<p>The heat change measured by ITC includes contributions from all processes occurring in the solution (C)</p> Signup and view all the answers

What is a disadvantage of ITC regarding speed and sample amount?

<p>The technique can be slow and requires relatively large amounts of sample (B)</p> Signup and view all the answers

Is ITC suitable for high-throughput screening?

<p>No, it is not suitable (B)</p> Signup and view all the answers

Is ITC suitable for high-throughput screening?

<p>No, it is not suitable (B)</p> Signup and view all the answers

Should scientists use ITC in conjunction with other methods?

<p>Yes, it is important for scientists to use ITC in conjunction with other methods. (C)</p> Signup and view all the answers

Should scientists use ITC in conjunction with other methods?

<p>Yes, it is important for scientists to use ITC in conjunction with other methods. (C)</p> Signup and view all the answers

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