Unlocking the Secrets of Restriction Endonucleases

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Which of the following best describes restriction endonucleases?

Enzymes that recognize specific short stretches of nucleotides and cut DNA at those sites (correct)

Enzymes that recognize specific short stretches of nucleotides and join DNA at those sites

Enzymes that recognize specific long stretches of nucleotides and cut DNA at those sites

Enzymes that recognize specific long stretches of nucleotides and join DNA at those sites

What is competence in the context of molecular biology?

The ability of a donor cell to take up extracellular DNA from the environment

The ability of a recipient cell to secrete DNA into the environment

The ability of a recipient cell to take up extracellular DNA from the environment (correct)

The ability of a donor cell to secrete DNA into the environment

What is the purpose of blue-white screening in molecular cloning?

To identify bacterial colonies that have undergone mutation

To identify bacterial colonies that have not taken up a recombinant plasmid

To identify bacterial colonies that have successfully taken up a recombinant plasmid (correct)

To identify bacterial colonies that have not undergone mutation

Which of the following best describes restriction endonucleases?

Enzymes that recognize specific short stretches of nucleotides Signup and view all the answers

What is the purpose of blue-white screening in molecular cloning?

To identify recombinant plasmids Signup and view all the answers

What is the role of competence in molecular biology?

Ability of a cell to take up extracellular DNA Signup and view all the answers

What are the steps involved in molecular cloning?

Isolation of gene, cutting with restriction enzymes, joining with ligase, transformation, screening Signup and view all the answers

How can cells be made competent for transformation?

By chemical means: CaCl2 or by electrical means: electroporation Signup and view all the answers

What is the function of the lacZ reporter gene in blue-white screening?

To produce blue colonies Signup and view all the answers

What is genetic engineering?

The alteration of genetic material in an organism to change its traits Signup and view all the answers

What is the role of competence factor in Streptococcus pneumoniae?

To induce the competence state in recipient cells Signup and view all the answers

How do Haemophilus influenzae cells become competent?

When the culture is switched from rich to minimal growth medium Signup and view all the answers

What is the purpose of screening for recombinant plasmids in molecular cloning?

To identify cells that have taken up the recombinant plasmid Signup and view all the answers

Unlocking the Secrets of Restriction Endonucleases

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Questions and Answers

Which of the following best describes restriction endonucleases?

What is competence in the context of molecular biology?

What is the purpose of blue-white screening in molecular cloning?

Which of the following best describes restriction endonucleases?

What is the purpose of blue-white screening in molecular cloning?

What is the role of competence in molecular biology?

What are the steps involved in molecular cloning?

How can cells be made competent for transformation?

What is the function of the lacZ reporter gene in blue-white screening?

What is genetic engineering?

What is the role of competence factor in Streptococcus pneumoniae?

How do Haemophilus influenzae cells become competent?

What is the purpose of screening for recombinant plasmids in molecular cloning?

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