Unlocking the Secrets of Restriction Endonucleases
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Questions and Answers

Which of the following best describes restriction endonucleases?

  • Enzymes that recognize specific short stretches of nucleotides and cut DNA at those sites (correct)
  • Enzymes that recognize specific short stretches of nucleotides and join DNA at those sites
  • Enzymes that recognize specific long stretches of nucleotides and cut DNA at those sites
  • Enzymes that recognize specific long stretches of nucleotides and join DNA at those sites

    • What is competence in the context of molecular biology?

  • The ability of a donor cell to take up extracellular DNA from the environment
  • The ability of a recipient cell to secrete DNA into the environment
  • The ability of a recipient cell to take up extracellular DNA from the environment (correct)
  • The ability of a donor cell to secrete DNA into the environment

    • What is the purpose of blue-white screening in molecular cloning?

  • To identify bacterial colonies that have undergone mutation
  • To identify bacterial colonies that have not taken up a recombinant plasmid
  • To identify bacterial colonies that have successfully taken up a recombinant plasmid (correct)
  • To identify bacterial colonies that have not undergone mutation

    • Which of the following best describes restriction endonucleases?

    <p>Enzymes that recognize specific short stretches of nucleotides</p> Signup and view all the answers

    What is the purpose of blue-white screening in molecular cloning?

    <p>To identify recombinant plasmids</p> Signup and view all the answers

    What is the role of competence in molecular biology?

    <p>Ability of a cell to take up extracellular DNA</p> Signup and view all the answers

    What are the steps involved in molecular cloning?

    <p>Isolation of gene, cutting with restriction enzymes, joining with ligase, transformation, screening</p> Signup and view all the answers

    How can cells be made competent for transformation?

    <p>By chemical means: CaCl2 or by electrical means: electroporation</p> Signup and view all the answers

    What is the function of the lacZ reporter gene in blue-white screening?

    <p>To produce blue colonies</p> Signup and view all the answers

    What is genetic engineering?

    <p>The alteration of genetic material in an organism to change its traits</p> Signup and view all the answers

    What is the role of competence factor in Streptococcus pneumoniae?

    <p>To induce the competence state in recipient cells</p> Signup and view all the answers

    How do Haemophilus influenzae cells become competent?

    <p>When the culture is switched from rich to minimal growth medium</p> Signup and view all the answers

    What is the purpose of screening for recombinant plasmids in molecular cloning?

    <p>To identify cells that have taken up the recombinant plasmid</p> Signup and view all the answers

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