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Questions and Answers
Which of the following best describes restriction endonucleases?
Which of the following best describes restriction endonucleases?
- Enzymes that recognize specific short stretches of nucleotides and cut DNA at those sites (correct)
- Enzymes that recognize specific short stretches of nucleotides and join DNA at those sites
- Enzymes that recognize specific long stretches of nucleotides and cut DNA at those sites
- Enzymes that recognize specific long stretches of nucleotides and join DNA at those sites
What is competence in the context of molecular biology?
What is competence in the context of molecular biology?
- The ability of a donor cell to take up extracellular DNA from the environment
- The ability of a recipient cell to secrete DNA into the environment
- The ability of a recipient cell to take up extracellular DNA from the environment (correct)
- The ability of a donor cell to secrete DNA into the environment
What is the purpose of blue-white screening in molecular cloning?
What is the purpose of blue-white screening in molecular cloning?
- To identify bacterial colonies that have undergone mutation
- To identify bacterial colonies that have not taken up a recombinant plasmid
- To identify bacterial colonies that have successfully taken up a recombinant plasmid (correct)
- To identify bacterial colonies that have not undergone mutation
Which of the following best describes restriction endonucleases?
Which of the following best describes restriction endonucleases?
What is the purpose of blue-white screening in molecular cloning?
What is the purpose of blue-white screening in molecular cloning?
What is the role of competence in molecular biology?
What is the role of competence in molecular biology?
What are the steps involved in molecular cloning?
What are the steps involved in molecular cloning?
How can cells be made competent for transformation?
How can cells be made competent for transformation?
What is the function of the lacZ reporter gene in blue-white screening?
What is the function of the lacZ reporter gene in blue-white screening?
What is genetic engineering?
What is genetic engineering?
What is the role of competence factor in Streptococcus pneumoniae?
What is the role of competence factor in Streptococcus pneumoniae?
How do Haemophilus influenzae cells become competent?
How do Haemophilus influenzae cells become competent?
What is the purpose of screening for recombinant plasmids in molecular cloning?
What is the purpose of screening for recombinant plasmids in molecular cloning?
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