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What is the primary purpose of homologous chromosomes during meiosis?

  • To control the timing of cell division
  • To produce identical daughter cells
  • To prevent mutation during cell replication
  • To promote genetic variation through crossover (correct)
  • Which phase of meiosis is characterized by the formation of chiasmata?

  • Prophase I (correct)
  • Metaphase I
  • Anaphase II
  • Telophase II
  • What is a consequence of a cell moving past the G1 checkpoint without repairing damaged DNA?

  • The damaged DNA will be replicated (correct)
  • The cell will stop dividing indefinitely
  • The cell will undergo programmed cell death
  • The cell will enter a growth phase
  • Which protein complex is essential for a cell to transition from G2 to mitosis?

    <p>Maturation Promoting Factor (MPF)</p> Signup and view all the answers

    How does genetic variation occur during meiosis?

    <p>Through both crossover and independent assortment</p> Signup and view all the answers

    During what process are four genetically distinct daughter cells formed?

    <p>Meiosis II</p> Signup and view all the answers

    What best describes independent assortment in meiosis?

    <p>Chromosomes align randomly at the metaphase plate</p> Signup and view all the answers

    Which of the following statements about sister chromatids is correct?

    <p>Sister chromatids are identical copies of a chromosome</p> Signup and view all the answers

    What explains the presence of 35-45 tRNAs for 61 codons?

    <p>Each tRNA can recognize multiple codons for the same amino acid due to wobble pairing</p> Signup and view all the answers

    Which type of mutation is responsible for sickle cell anemia?

    <p>Missense</p> Signup and view all the answers

    What occurs during the interphase of the cell cycle?

    <p>DNA replication in the S phase</p> Signup and view all the answers

    What is the role of the centromere in cell division?

    <p>Serves as a platform for kinetochore attachment</p> Signup and view all the answers

    What describes the state of chromosomes during anaphase?

    <p>Chromosomes are reeling in towards the poles</p> Signup and view all the answers

    Which of the following processes is NOT associated with cell division?

    <p>RNA splicing</p> Signup and view all the answers

    Which statement best describes sister chromatids?

    <p>Identical copies of a chromosome formed during DNA replication</p> Signup and view all the answers

    During which phase of the cell cycle do cells prepare for mitosis?

    <p>G2 phase</p> Signup and view all the answers

    During anaphase, how do chromosomes behave?

    <p>They walk along the microtubules toward the centrosomes.</p> Signup and view all the answers

    In which phase do chromosomes first visibly condense?

    <p>Prophase</p> Signup and view all the answers

    What role does the Rb protein play in the cell cycle?

    <p>It inhibits cell division.</p> Signup and view all the answers

    What is the main function of Cyclin D and CDK4/6 complexes during the cell cycle?

    <p>To remove the Rb protein and bypass the G1 checkpoint.</p> Signup and view all the answers

    What characteristic defines proto-oncogenes?

    <p>They regulate normal cell division.</p> Signup and view all the answers

    How do oncogenes contribute to cancer development?

    <p>They cause abnormal and rapid cell division.</p> Signup and view all the answers

    What marks the M checkpoint during cell division?

    <p>Ensuring kinetochores are attached at the metaphase plate.</p> Signup and view all the answers

    What is the role of tumor suppressor genes (TSG) like p53?

    <p>To initiate DNA repair and regulate the cell cycle.</p> Signup and view all the answers

    What is the significance of hyperactive Ras protein mutations?

    <p>They lead to uncontrolled cell division even in the absence of growth factors.</p> Signup and view all the answers

    How do cancer cells exhibit loss of anchorage dependence?

    <p>They can metastasize through the circulatory system.</p> Signup and view all the answers

    Study Notes

    Midterm 2 Review - BIO 93 TRIO

    • F24 BioSci Peer Tutors: Victoria Vo, Sophie Cohen, Charlotte Phan, Hannah Song

    Lecture 10: ATP

    • Hydrolysis of ATP is exergonic.
    • Hydrolysis of ATP can be coupled with endergonic reactions to make them spontaneous.
    • Energy is stored in the phosphate bonds of ATP.
    • Repulsion between negatively charged phosphate groups in ATP allows easy energy release when broken.

    Lecture 10: Enzymes

    • Catalytic protein.
    • Greatly affected by pH and temperature. Optimal human enzyme temperature is 37°C.
    • General Structure:
      • Substrate: molecule acted upon by the enzyme
      • Active site: area of enzyme where reaction occurs
      • Enzyme-substrate complex: the combination of enzyme and substrate
      • Enzyme: catalyzes a reaction
      • Products: result of enzyme activity
    • Steps of Enzyme Action:
      • 1. Substrate binds to enzyme at active site.
      • 2. Induced fit.
      • 3. Enzyme performs catalytic activities.
      • 4. Releases the product.
    • Inhibition of Enzymes:
    • Competitive inhibition
      • Inhibitor
      • Enzyme
      • Substrate
    • Noncompetitive inhibition
      • Inhibitor
      • Enzyme
      • Substrate
      • Allosteric site
    • Enzyme-Catalyzed Reaction:
      • Lowered activation energy
      • AG does not change
      • Enzyme is not consumed in reaction
      • Speeds up reaction

    Poll Question (Page 3)

    • Which one of these processes DO NOT require the use of energy?
      • a) Endocytosis and exocytosis
      • b) Activation of TKR (Tyrosine Kinase Receptors)
      • c) Activation of GPCR (G-protein coupled receptors)
      • d) Na+/K+ pump
      • e) Hydrolysis of ATP

    Poll Question (Page 6)

    • Which of the following PAIRS of statements are true?
      • a) Noncompetitive inhibitors bind to the allosteric site.
      • b) Enzyme inhibitors increase the activation energy of a reaction.
      • c) Competitive inhibitors bind to both allosteric and active site.
      • d) Although substrates can bind to the allosteric site, it mostly binds to the active site.
      • e) Inhibitors and activators can bind to the allosteric site or active site, but substrates can only bind to the active site.

    Lecture 11: Cellular Respiration

    • Glycolysis:
      • 1 glucose molecule -> 2 pyruvates -> 2 ATP
    • Intermediate Step (Pyruvate Oxidation):
      • No ATP made.
    • Citric Acid Cycle:
      • active transport via MPC
      • 2 acetyl coA + 6 NADH + 2 FADH
    • Oxidative Phosphorylation:
      • 26-28 ATP

    Poll Question (Page 8)

    • Which steps of cellular respiration include substrate level phosphorylation?
      • a) Glycolysis and Pyruvate Oxidation
      • b) Pyruvate Oxidation and Kreb Cycle
      • c) The ETC and Chemiosmosis
      • d) Glycolysis and the Citric Acid Cycle

    Poll Question (Page 9)

    • At what step of cellular respiration does the body diverge to fermentation if oxygen is not present?
    • a) Before glycolysis
    • b) Before pyruvate oxidation
    • c) Before the Kreb cycle
    • d) Before Oxidative Phosphorylation

    Lecture 11: Fermentation

    • Lactic Acid Fermentation:
      • Muscle cells
      • Lactate exported to liver.
      • Reformed into glucose.
      • 2 ATP per glucose.
      • No CO2 released.
    • Alcohol Fermentation:
      • Bacteria and Yeast
      • Release 2 CO2/glucose.
      • 2 ATP/glucose.

    Lecture 12: Photosynthesis

    • 6CO2 + 6H2O → sunlight → C6H12O6 + 6O2
    • Site of photosynthesis: Chloroplast
    • 2 parts: Light reactions and Calvin cycle
    • Photosynthesis is a REDOX reaction.
      • H2O oxidized → O2
      • CO2 reduced → sugar

    Pt. 1: Light Reaction

    • Light absorption directly correlates to action spectrum
    • Light reactions take place in the thylakoid membrane
    • Photosystems II and I convert light energy into chemical energy
    • Uses light energy with photosystems to excite electrons.
    • Electron transport chain moves electrons into thylakoid space which creates ATP and NADPH.
    • Water is split and oxygen is released.

    Pt. 2: Calvin Cycle

    • Input: CO2, NADPH, ATP.
    • Output: NADP+, ADP + Pi, G3P.
    • 3 phases: Carbon fixation, Reduction, Regeneration.

    Poll Question (Page 15)

    • If ATP synthase on the thylakoid membrane was inhibited, what would happen to the pH of the thylakoid space?
      • a) Increase
      • b) Decrease
      • c) Stay around same
      • d) First increase, then decrease

    Poll Question (Page 16)

    • If rubisco became denatured in Bob's stroma, which of the following stages in the Calvin cycle would be disrupted?
      • a) Carbon reduction
      • b) Carbon fixation
      • c) RuBP regeneration
      • d) G3P to glucose process

    Lecture 13: The Molecular Basis of Inheritance

    • Griffith Experiment:
      • R strain (nonvirulent) → mouse lives
      • S strain (virulent) → mouse dies
      • Heat-killed S strain → mouse lives
      • Mix of heat-killed S strain and living R strain → mouse dies
      • Concluded that a transforming principle moves from dead S to live R
    • Hershey-Chase Experiment:
      • Proteins were stained with radioactive sulfur.
      • Nucleic acids were stained with radioactive phosphorus.
      • Radioactive phosphorus was found in bacteria pellet.
      • DNA is the hereditary material.
    • DNA Structure:
      • of adenine = # of thymine

      • of cytosine = # of guanine

      • A pairs with T; C Pairs with G
      • Purines must pair with pyrimidines
      • A and T have two hydrogen bonds, C and G have three.
      • X-ray crystallography determined structure of DNA
      • DNA is a double helix with two strands and hydrophobic interior (bases) and hydrophilic exterior (phosphate backbone).

    Poll Question (Page 22)

    • In a single strand of DNA, guanine comprises 27%. How much thymine is present?
      • a) 73%
      • b) 36.5%
      • c) 27%
      • d) Impossible to determine

    Poll Question (Page 23)

    • What is the arrow pointed towards in this image?
      • a) Origin of replication
      • b) Replication bubble
      • c) Replication fork
      • d) Answers A and C

    Poll Question (Page 24)

    • Which image displays the correct DNA replication method?
      • a) [Image of DNA replication with each strand separate and mixed]
      • b) [Image of DNA replication with the strands shown as one and splitting]
      • c) [Image of DNA replication with strands splitting and copying and showing mixture]

    Poll Question (Page 25)

    • Which of the following DNA molecules has a site that is most likely to be an origin of replication?
      • Provides possible answers and asks students to identify which one is more likely
      • Look for sequences with repeating base pairs like A-T or G-C

    DNA Replication and Repair (Lecture 14)

    • DNA is semiconservative.
    • DNA replicates in the 5'→ 3' direction.
    • Replication begins at origins of replication.
    • Replication proteins are crucial to DNA replication (helicase, polymerase, SSBP, etc).

    DNA Synthesis (Page 28)

    • DNA polymerase can only add new nucleotides to the 3' hydroxyl group of existing DNA strands
    • Replication is a 5' → 3' process.

    Replication Proteins (Page 29)

    • List of proteins including: helicase, SSB, topoisomerase, primase, DNA polymerase III, DNA polymerase I, and ligase with detailed function of each.

    DNA Proofreading and Repair (Lecture 14)

    • Mismatch repair of DNA
      • Repair enzymes correct errors.
    • Nucleotide excision repair - Repairs damaged DNA
    • Shows possible diagrams to identify what is occurring

    Poll Question (Page 32)

    • During DNA replication in a human cell, helicase malfunctions. What effect would this have on the replication process?
      • a) The replication fork would continue but leading strands would be synthesized at a slower rate.
      • b) Primase would synthesize primers but they would not bind to DNA due to the lack of exposed single strands.
      • c) DNA polymerase III would be unable to synthesize new DNA strands because the parental DNA remains double stranded.

    Poll Question (Page 33)

    • If Peter the Anteater introduces a mismatch base during synthesis, what is the most likely consequence if mismatch repair is functioning normally?
      • a) The mismatch will be recognized and corrected to the correct base pair.
      • b) DNA ligase will detect the mismatch and remove the incorrect nucleotide.
      • c) The mismatch will remain uncorrected, potentially leading to a permanent mutation

    Poll Question (Page 34)

    • A scientist is studying DNA synthesis with a mutation in DNA Polymerase III. What would likely happen to the replication process?
      • a) DNA synthesis on the leading strand will stop, but lagging strand synthesis will continue normally.
      • b) DNA synthesis will start at the origin but is unable to elongate effectively.
      • c) Okazaki fragments would be directly joined together.

    Poll Question (Page 35)

    • What does Drug C most likely inhibit? (Requires information from a given graph).

    Lecture 15: Transcription

    • Protein links genotype and phenotype: Genes produce proteins via transcription and translation.
    • Transcription: production of mRNA from DNA.
    • Involves RNA polymerase, binding to promoter region of a gene.
    • RNA polymerase separates DNA strands and produces a transcribed RNA strand complementary to one strand of the DNA.
    • Translation: formation of a protein sequence based on mRNA, occurs at ribosomes.
    • Base pairs code for amino acids: 3 base pairs code for an amino acid (codon).

    Prokaryotic vs. Eukaryotic Transcription and Translation

    • Prokaryotes have coupled transcription and translation (transcription & translation happen at the same time).
    • Eukaryotes have separated transcription and translation; transcription occurs in the nucleus and translation occurs mainly at ribosomes in the cytoplasm.

    Transcription (Initiation, Elongation, Termination) (Page 40)

    • Summarize each phase, including actions of RNA polymerase.

    RNA Processing (Page 45)

    • Prokaryotes: RNA transcripts act as mRNA right away. - Functions: - Facilitate mRNA export. - Protect RNA from degradation. - Helps attach ribosomes for translation.
    • Eukaryotes: Eukaryotes need modifications to pre-mRNA before functioning as mRNA.
      • Involves RNA splicing by spliceosomes.

    tRNA (Page 46):

    • Function: Each tRNA carries a specific amino acid depending on its anticodon.
      • tRNA and aminoacyl-tRNA synthetase match each tRNA to its specific amino acid.
    • Structure of tRNA - explains the structure with H bonding, for example, important sections like T-loop, anticodon loop

    Translation (page 47 and 48):

    • Summarize steps of translation (Initiation, Elongation, Termination).

    Protein Location (Translation) (Page 49):

    • How SRP and signal peptide work within protein translation, including the roles of signal-cleaving enzyme and the ER membrane pore.

    Point Mutations (Page 50):

    • Types of point mutations (Nucleotide pair substitution, insertions, and deletions).
    • Show possible examples.

    Poll Question (Page 51)

    • Why is there on average 35-45 tRNAs for 61 codons?
      • a) tRNAs are not involved in protein synthesis, so the number does not correlate with amino acid count.
      • b) There are multiple anticodons for each amino acid.
      • c) Each tRNA can recognize multiple codons for the same amino acid because of wobble pairing.
      • d) There's only one codon for each amino acid.

    Poll Question (Page 52)

    • What kind of mutation occurred for sickle cell anemia?
      • a) Nonsense
      • b) Missense
      • c) Silent
      • d) Insertion

    Lecture 17: Cell Division Pt. 1

    • Where do we see cell division?
      • Reproduction
      • Tissue renewal and repair (e.g., wound healing)
      • Growth and development

    Chromosome Structure (Page 55):

    • Structure of chromosomes including:
      • A chromosome is a single DNA molecule made of proteins wrapped around strands of DNA.
      • Sister chromatids are identical copies of a chromosome.
      • Centromeres are platforms for kinetochores (spindle microtubules attachment points)

    Cell Cycle: 2 Main Phases (Page 56):

    • Interphase:
      • G1: Cell physically grows larger, copies organelles, synthesizes necessary macromolecules.
      • S: DNA replication occurs.
      • G2: More cell growth and preparation for mitosis.
    • M Phase: Mitotic phase which includes series of activities leading to forming two daughter cells (including Prophase, Prometaphase, Metaphase, Anaphase, Telophase, and Cytokinesis).

    Cell Cycle- to M Phase (Page 57):

    • Sequence of steps occurs during the phases of mitosis detailed in the diagram.

    Poll Question (Page 58 and 59):

    • Given this diagram, during anaphase are chromosomes “reeled in” or “walk in”?
      • a) Reeled in
      • b) Walked in

    Poll Question (Page 60):

    • In what phase do chromosomes condense? a) Prophase b) Prometaphase c) Metaphase d) G2 phase

    Lecture 18: Cell Division Pt. 2

    • Checkpoints:
      • G1 checkpoint: Cell size, nutrients, growth factors, DNA damage, hormones and signals.
      • G2 checkpoint: DNA damage, DNA replication completeness, Sufficient MPF complexes.
      • M checkpoint: Ensures kinetochores are attached to chromosomes at metaphase plate during prometaphase.

    Proto-oncogenes and Oncogenes (Page 63):

    • Proto-oncogene: Normal gene role in regulating cell division and growth.
      • Encode proteins to stimulate cell division, inhibit cell differentiation, and halt cell death.
    • Oncogene: Mutated version of proto-oncogene, causes abnormal and rapid cell division (cancer).
      • Increased cell division, decreased differentiation, and inhibition of cell death.
    • Explains hallmarks of cancer cells.

    Regulating Genes of Cell Division (page 64):

    • Explains Ras proteins, mutation of ras, and function of tumor suppressor genes.

    Meiosis (Page 65):

    • Briefly describe the process of meiosis in this section including meiosis 1 and 2. - Each homologous chromosome duplicates, forms two sets of chromosomes with two sister chromatids. - Chiasmata formation. Crossover and separation of homologous chromosomes; Formation of two non-identical daughter cells. - Separation of sister chromatids happens

    Genetic Variations (Page 66):

    • Includes Crossover, Independent Assortment of Chromosomes, and Random Fertilization.
      • Explains how and why genetic variations occur during cell reproduction.

    Poll Question (Page 67):

    • Which of the following is the correct definition of a homologous chromosome?
      - a) A chromosome not directly involved in sex determination of offspring - b) A pair of sister chromatids of the same chromosome - c) A pair of chromosomes that have the same length and gene loci - d) The chromosome responsible for determining the sex of the offspring

    Question (Page 68):

    • If a cell bypasses the G1 checkpoint without repairing damaged DNA, what could be the consequence?
      • The cell will replicate damaged DNA and increase risk of mutations.

    Question (Page 69):

    • Which protein complex is crucial for the cell to pass from the G2 checkpoint into mitosis?
      • Cyclin E-Cdk2 complex
      • Maturation Promoting Factor (MPF)

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