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Questions and Answers

What type of organisms utilize sunlight and carbon dioxide to produce their own food?

  • Extremophiles
  • Phototrophs (correct)
  • Chemotrophs
  • Heterotrophs
  • Which of the following is a life strategy that involves the use of pre-formed organic compounds for energy?

  • Photoheterotroph
  • Chemoheterotroph (correct)
  • Chemoautotroph
  • Photoautotroph
  • What is the primary role of microbes in cancer therapeutics?

  • Produce vaccines
  • Enhance photosynthesis
  • Actively target cancerous cells (correct)
  • Degrade plastics
  • Which group of microbes is known for thriving in extremely high-temperature environments?

    <p>Hyperthermophiles</p> Signup and view all the answers

    Which process does NOT involve the use of restriction enzymes?

    <p>Cellular respiration</p> Signup and view all the answers

    Study Notes

    Global Impact of Microbes

    • Microbes play a crucial role in the environment, impacting various processes like plastic degradation and phytoplankton populations in seas.
    • Microbes are also key components in technology, such as polymerase chain reaction (PCR), restriction enzymes, and CRISPR-Cas systems.
    • Microbes are integral parts of medicine encompassing cancer therapeutics and antibiotics.
    • Microbes are the foundation of all life. Following different energy sources, they are classified as either phototrophs or chemotrophs. They are likewise classified based on their carbon sources: autotrophs and heterotrophs.
    • Different classifications of microbes exist based on their energy and carbon sources, such as photoautotrophs, photoheterotrophs, chemoautotrophs, and chemoheterotrophs.
    • Specific examples of microbes include plants, algae, cyanobacteria, extremophiles, purple/green non-sulfur bacteria, humans, and animals.

    Microbial Impact on the Environment

    • Plastic pollution has substantially increased since 1950.
    • Microbes play a role in plastic degradation.
    • Microbes like those in the microbiome can be platforms for colonizing plastics, affecting their toxic effects, and providing carbon sources.

    Microbial Life Strategies

    • All life begins with microorganisms.
    • Microbes can utilize either light or chemical oxidation as their energy source, classifying them into phototrophs and chemotrophs, respectively.
    • Microbes can obtain carbon from either inorganic sources (CO2), classifying them as autotrophs, or from organic compounds, classifying them as heterotrophs.
    • Microbes utilize these sources of energy and carbon in various ways, like photoautotrophs, photoheterotrophs, chemoautotrophs, and chemoheterotrophs.

    Microbial Adaptations

    • Microorganisms have varying optimal growth temperatures, ranging from psychrophiles, that thrive in cold temperatures, to hyperthermophiles, which thrive in extreme heat conditions.
    • Cell membranes of microbes change their fatty acid composition depending on the temperature to adapt to different conditions
    • Important factors in microbial survival and adaptation are cryoprotectants, cold/heat shock proteins
    • Cell membrane composition has a high content of saturated fat to form a solid at extreme temperatures

    Microbial Distribution

    • Microbes exist in various environments including marine environments, caves, plants, animals, soil, and the atmosphere.

    Marine Environment

    • Marine environments encompass various zones, including neritic and oceanic zones, characterized by different water conditions.
    • Neritic zones are nutrient-rich with moderately high temperatures and pressures.
    • Oceanic zones display higher pressures and lower temperatures and nutrients.
    • Photosynthetic organisms and diverse marine life characterize neritic zones.
    • Chemo- and phototrophs exhibit in oceanic zone.

    Ocean Plankton

    • Oceanic plankton, crucial for oxygen production, account for 50-80% of Earth's oxygen.
    • Specific plankton like Prochlorococcus are responsible for a significant portion (20%) of Earth's oxygen production.
    • Synechococcus and Prochlorococcus are key players in marine carbon fixation.

    Microbes in Technology

    • The field of molecular biology has been transformed by the application of DNA polymerase I from Thermus aquaticus (Taq polymerase).
    • This led to the development of the polymerase chain reaction (PCR).
    • Restriction enzymes are proteins produced by bacteria that cut DNA at specific sites.
    • CRISPR-Cas systems have applications in gene editing and biotechnology, offering resistance to viruses or plasmids containing related sequences, matching the spacers.
    • Applications of restriction enzymes include recombinant gene technology.

    Microbes in Medicine

    • Microbes contribute significantly to diverse diseases, including cancer.
    • Viruses like HPV can lead to various cancers.
    • Microbes are used in the production of recombinant vaccines through expressing antigens in yeast cells. This approach has been applied to hepatitis B, human papillomaviruses (HPVs), and SARS-Cov-2.
    • Recombinant viruses are also used as vaccines

    Antibiotics

    • Broad-spectrum antibiotics inhibit cell wall biosynthesis.
    • Antibiotics are derived from microbes like Streptomyces griseus

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