17 Questions
What is a key characteristic of prokaryotic DNA?
No introns
Where is prokaryotic DNA typically located in the cell?
Freely in the cytoplasm
Which statement is true regarding the number of DNA copies in prokaryotes?
Single copy (haploid)
Which element is present in prokaryotic DNA but absent in eukaryotic DNA?
Plasmids
What distinguishes prokaryotic DNA from eukaryotic DNA in terms of repetitive DNA and introns?
Contains little repetitive DNA and no introns
What is the main difference between conjugative and non-conjugative plasmids?
Conjugative plasmids can be mobilized, while non-conjugative plasmids cannot be mobilized
What is the defining characteristic of a shuttle vector?
Shuttle vectors can propagate in two different host species
In the context of plasmids, what is the role of transposons?
Transposons are capable of moving within DNA to different locations
What distinguishes natural plasmids from artificial plasmids?
Natural plasmids are designed artificially
Which characteristic represents stringent plasmids?
Plasmids that cannot be maintained in the same cell
What is the primary function of plasmids in molecular biology?
To act as vectors for gene therapy and DNA vaccines
What is the function of Insertion sequences (IS) in transposable elements?
Encode only proteins needed for its own transposition
Which type of transposable element contains two IS at both ends and a central piece of DNA encoding for antibiotic resistance or virulence factors?
Composite transposons (Tn)
What do Non-composite transposons lack at their ends?
Insertion sequences (IS)
Which of the following best describes a shuttle vector plasmid?
Circular extrachromosomal DNA capable of moving between prokaryotic and eukaryotic cells
What is the primary difference between prokaryotic and eukaryotic DNA replication?
Prokaryotic DNA replication occurs in the cytoplasm, while eukaryotic DNA replication occurs in the nucleus
Why did the 9-year-old girl not respond to ampicillin treatment despite having previously improved with it?
The bacteria developed resistance due to the presence of Tn3 transposon
Test your knowledge on the structure of transposable elements including Insertion Sequences (IS), Composite Transposons (Tn), and Non-composite transposons. Learn about the characteristics and examples of each type of transposable element.
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