Transport Proteins and Mechanisms Quiz

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Questions and Answers

What is the primary function of channels in transport proteins?

  • Use ATP to transport substances against their gradient
  • Bind to substances and change their conformation
  • Provide a barrier to prevent ion flow
  • Facilitate the diffusion of specific ions (correct)

Which type of channel is opened by mechanical stimuli?

  • Mechanically-gated channels (correct)
  • Ligand-gated channels
  • Second messenger gated channels
  • Voltage-gated channels

How do pumps differ from carriers in terms of energy use?

  • Both use energy but pumps are specific to ions only
  • Pumps rely on hydrolysis of ATP for transport, while carriers do not (correct)
  • Carriers can use ATP indirectly while pumps cannot
  • Pumps do not require energy, carriers do

What defines a uniport transporter?

<p>Transports a single substance along its gradient (A)</p> Signup and view all the answers

What is the function of a symport transport mechanism?

<p>Simultaneously transports multiple substances in the same direction (C)</p> Signup and view all the answers

What role does the sodium-potassium pump play in cellular function?

<p>Moves sodium and potassium ions against their gradients (A)</p> Signup and view all the answers

Which statement about antiporters is true?

<p>They transport substances in opposite directions across the membrane (D)</p> Signup and view all the answers

What distinguishes ligand-gated channels from other types of channels?

<p>They require the binding of specific chemicals to open (A)</p> Signup and view all the answers

What term describes the RNA that is synthesized and immediately undergoes processing before being released into the cytoplasm?

<p>pre-mRNA (B)</p> Signup and view all the answers

Which process involves the conversion of the linear sequence of codons on mRNA into a polypeptide chain?

<p>Translation (B)</p> Signup and view all the answers

What is the role of tRNA in the translation process?

<p>To decode mRNA codons into amino acids (C)</p> Signup and view all the answers

Which of the following statements accurately describes the concept of normalcy in biological systems?

<p>It is inappropriate to define normal range as a single value (C)</p> Signup and view all the answers

What is the significance of post-translational modification in protein synthesis?

<p>It leads to the formation of functional molecules. (C)</p> Signup and view all the answers

What is the approximate percentage of total body water (% TBW) in a normal adult woman?

<p>55 - 60 (B)</p> Signup and view all the answers

Which of the following statements about total body water (TBW) in infants is true?

<p>Infants have a higher TBW than adult women (B)</p> Signup and view all the answers

Which of these is the only naturally regulated entry route for extracellular fluid (ECF)?

<p>Mouth and gut (D)</p> Signup and view all the answers

How is the distribution of extracellular fluid (ECF) divided?

<p>Two compartments: Interstitial fluid (ISF) and intravascular fluid (IVF) (C)</p> Signup and view all the answers

What is the approximate lean (fat-free) body mass for a normal adult man?

<p>25 kg (A)</p> Signup and view all the answers

What is the primary difference between osmolarity and osmolality?

<p>Osmolarity measures osmotically active particles by volume, whereas osmolality measures by mass. (C)</p> Signup and view all the answers

At a pH of 7.4, what is the concentration of H+ ions in the body fluids?

<p>4 x 10⁻⁸ mol/l (A)</p> Signup and view all the answers

What is a primary function of cell adhesion molecules (CAMs)?

<p>To provide intercellular connections and stability (C)</p> Signup and view all the answers

How does the intracellular compartment's osmolality compare to that of the extracellular compartment?

<p>Intracellular osmolality is lower by a factor of two. (A)</p> Signup and view all the answers

Which type of junction is responsible for the selective permeability in epithelial tissues?

<p>Tight junctions (D)</p> Signup and view all the answers

Which of the following families do not belong to the classifications of CAMs?

<p>Claudins (B)</p> Signup and view all the answers

Which statement correctly describes a factor affecting osmolarity?

<p>Osmolarity can change with varying solute concentrations and temperature. (D)</p> Signup and view all the answers

What effect would a breakdown of the body's homeostatic mechanisms most likely indicate?

<p>Significant fluctuations in internal conditions. (A)</p> Signup and view all the answers

What critical role do CAMs play in inflammation?

<p>Enabling adhesion of neutrophils to endothelial cells (A)</p> Signup and view all the answers

What is the primary function of receptors in the cell membrane?

<p>To provide binding sites for specific molecules (D)</p> Signup and view all the answers

In the context of the cell membrane, what does endocytosis refer to?

<p>Movement of large molecules into the cell (C)</p> Signup and view all the answers

Which types of junctions primarily contribute to the mechanical strength of tissues?

<p>Desmosomes and gap junctions (B)</p> Signup and view all the answers

Which function is not associated with exocytosis?

<p>Importing nutrients into the cell (B)</p> Signup and view all the answers

What is the primary function of microfilaments in the cell?

<p>They enable muscle contraction and cellular movement. (B)</p> Signup and view all the answers

Which molecular motor is primarily responsible for transporting substances toward the cell's nucleus?

<p>Dynein (A)</p> Signup and view all the answers

What do vaults primarily do within the cell?

<p>Facilitate the movement of mRNA and ribosomal subunits. (B)</p> Signup and view all the answers

What structural role do intermediate filaments play in cells?

<p>They provide resistance to external pressure. (D)</p> Signup and view all the answers

What component of the cell is responsible for carrying genetic information?

<p>Chromatin (A)</p> Signup and view all the answers

How do kinesin and dynein differ in their function within the cell?

<p>Kinesin moves cargo away from the nucleus, while dynein moves it towards the nucleus. (B)</p> Signup and view all the answers

What structure forms the mitotic spindle to move chromosomes during cell division?

<p>Microtubules (B)</p> Signup and view all the answers

What function does the nuclear membrane perform regarding molecular transport?

<p>It permits the passage of molecules as large as RNA. (B)</p> Signup and view all the answers

Flashcards

Integral (transmembrane) proteins

Proteins embedded within the cell membrane that facilitate the movement of molecules across the membrane.

Channels

A type of integral protein that forms a channel through the cell membrane, allowing specific ions to passively diffuse across the membrane.

Voltage-gated channels

Channels that can be opened or closed in response to changes in the electrical potential across the cell membrane.

Ligand-gated channels

Channels that are opened or closed by the binding of specific molecules called ligands, such as hormones or neurotransmitters.

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Second messenger gated channels

Channels that are opened or closed by the binding of second messenger molecules, such as cAMP or IP3, within the cell.

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Carriers

A type of integral protein that binds to specific molecules and facilitates their movement across the cell membrane.

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Facilitated diffusion carriers

Carriers that move molecules down their concentration gradient, facilitating their passive diffusion.

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Pumps

Carriers that move molecules against their concentration gradient, requiring energy from ATP hydrolysis.

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Cytoskeleton

The internal skeleton of a cell, made up of microtubules, microfilaments, and proteins. It provides structure, allows for shape changes, and enables movement.

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Vaults

Hollow, octagonal structures that resemble nuclear pores. They're thought to function as cellular transport vehicles, carrying cargo from the nucleus.

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Microtubules

Long, hollow cylinders composed of tubulin protein. They contribute to cell shape, organelle movement, and cell division.

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Microfilaments

Thin, solid fibers made of actin protein. They play roles in muscle contraction, cell movement, and maintaining cell shape.

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Molecular Motors

Proteins responsible for moving substances along microtubules. Examples include kinesin and dynein.

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Mitotic Spindle

A specialized structure made of microtubules, responsible for separating chromosomes during cell division.

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Nuclear Membrane (Envelope)

A double membrane surrounding the nucleus. It's dotted with pores that allow molecules to enter and exit.

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Chromatin

Made up of DNA and histone proteins. It condenses into chromosomes during cell division.

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Cell membrane receptors

Proteins embedded in the cell membrane that bind to specific molecules like hormones and drugs. They act like locks, recognizing and opening for specific keys.

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Cell membrane enzymes

Proteins in the cell membrane that accelerate chemical reactions at the membrane surface, acting like catalysts.

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Cell membrane antigens

Molecules on the cell membrane that identify the cell, like a name tag. They are important for the immune system and organ transplantation.

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Cell Adhesion Molecules (CAMs)

Proteins that connect cells to each other and to the surrounding matrix. They are important for tissue structure and function.

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Tight junction (zonula occludens)

A type of cell junction that seals cells together, preventing leaks between them. They are found in tissues like the intestinal lining and kidneys.

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Endocytosis

The movement of substances into the cell by engulfing them in a membrane vesicle. There are two types: pinocytosis (for liquids) and phagocytosis (for solids)

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Exocytosis

The movement of substances out of the cell by enclosing them in a membrane vesicle and releasing them outside.

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Adherens junctions

A type of cell connection that provides strong adhesion between cells. They are important for maintaining tissue integrity.

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Transcription

The process where DNA is used as a template to create a messenger RNA (mRNA) molecule.

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Post-transcriptional modification

The modification of the newly synthesized RNA (pre-mRNA) into a mature mRNA molecule before it leaves the nucleus.

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Translation

The process where mRNA is used as a template by ribosomes to synthesize protein.

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Post-translational modification

The final stage of protein synthesis, where the newly synthesized polypeptide chain undergoes chemical modifications to become a functional protein.

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Osmolarity

The concentration of osmotically active particles in a solution, expressed as osmoles or milliosmoles per liter of solution. It is affected by the volume of solutes and temperature.

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Osmolality

The concentration of osmotically active particles in a solution, expressed as osmoles or milliosmoles per kilogram of solvent (water). It is NOT affected by volume changes or temperature.

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pH

A measure of the hydrogen ion (H+) concentration in a solution. It is expressed on a logarithmic scale, with lower pH values indicating higher acidity.

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Osmotic Pressure

The difference in osmotic pressure between two solutions, often used to describe the tendency of water to move across a semipermeable membrane from a region of high water concentration to a region of low water concentration.

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Homeostasis

The ability of a cell to maintain a stable internal environment despite changes in the external environment.

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Body water percentage

Body water is the percentage of water in a person's body. It varies depending on age, gender and body fat.

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Extracellular fluid (ECF)

ECF is the fluid surrounding the cells, including interstitial fluid (ISF) and intravascular fluid (IVF).

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Intracellular fluid (ICF)

ICF is the fluid inside the cells. It is the largest fluid compartment in the body.

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ECF exit routes and regulation

The body loses ECF through four routes: skin, lungs, gut, and urinary tracts. But only the kidneys can regulate ECF loss precisely.

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Fluid compartment interactions

The body's fluid compartments are interconnected, with water constantly moving between them.

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Study Notes

Chapter 1: Cell Physiology

  • Cell structure and functions are fundamental to life
  • Body fluid compartments and their composition are crucial aspects of physiology
  • External and internal environments are in dynamic interaction
  • Changes in temperature, pH, and osmotic pressure affect cell activity

Cell Structure and Function

  • Cells are composed of water (70-80%), electrolytes (cations like K+, Na+, Mg++, anions like HCO₃⁻, PO₄³⁻), proteins (10-20%), lipids, and carbohydrates (10%)
  • Organelles are specialized structures within the cell
    • Membranous organelles include cell membrane, endoplasmic reticulum, mitochondria, Golgi apparatus, lysosomes, and peroxisomes.
    • Nonmembranous organelles include ribosomes, centrioles, vaults, microtubules, microfilaments
    • Nuclear membranous organelles include nuclear membrane (envelope), and nuclear nonmembranous include chromatin and nucleolus.
  • Inclusion bodies are temporary structures in cytoplasm (lipid droplets, glycogen granules, secretory granules).

Cell Membrane

  • The cell membrane (plasma membrane) is a dynamic, semi-permeable structure (7.5-10 nm thick)- a lipid bilayer with embedded proteins.
  • The bilayer has hydrophilic (polar) phosphate heads facing the aqueous environments and hydrophobic (nonpolar) fatty acid tails facing each other.
  • Integral proteins are embedded within the membrane or extending through both leaflets
  • Peripheral proteins are associated with the membrane surface, often bound to integral proteins.

Cytoplasmic Membranous Organelles

  • Golgi apparatus, rough endoplasmic reticulum, smooth endoplasmic reticulum, mitochondria, lysosomes are discussed.

Cytoplasmic Non-Membranous Organelles

  • Ribosomes, centrioles, microtubules and microfilaments are discussed

Functions of Cell Membrane

  • The cell membrane exhibits permeability and plays a role in movement of substances into and out of cells.
  • Membrane proteins act as channels and carriers, facilitating ion and molecule transport.
    • Channels permit passive diffusion through the membrane
    • Carriers bind substances and change shape to move them through.
    • Pumps actively move substances against their concentration gradient, requiring energy.

Cell Membrane Protein Types

  • Integral proteins are embedded within the membrane
  • Peripheral proteins are located on the membrane surface

Cell Adhesion Molecules (CAMs)

  • CAMs are proteins attaching cells to each other and the extracellular matrix.
  • Essential for tissue structure and function.

Intercellular Junctions

  • Tight junctions (zonula occludens) firmly bind cells, preventing leakage.
  • Desmosomes (spot-like) strengthen tissues
  • Gap junctions facilitate rapid transfer of ions and molecules between adjacent cells.

Endoplasmic Reticulum

  • Smooth ER synthesis of lipids and steroids, detoxification
  • Rough ER protein synthesis (ribosomes attached)

Golgi Apparatus

  • Involved in modifying, sorting, and packaging proteins and lipids for secretion or use within the cell.

Lysosomes

  • Contain digestive enzymes for waste breakdown and recycling.

Mitochondria

  • Site of energy production (ATP) through cellular respiration.
  • Contain their own DNA (mtDNA).

Nucleus

  • Contains genetic material (DNA) organized into chromosomes.
  • Nuclear envelope separates the nucleus from the cytoplasm.
  • Nucleolus is the site of ribosome synthesis.

Normal Values

  • Various physiological values (e.g., heart rate, body composition) vary based on individual factors (sex, age, body mass)
  • Wide ranges are often considered normal.

Total Body Water (TBW)

  • Percentage of TBW varies depending on age, sex, and body fat.
  • Infants have a high percentage of TBW compared to adults.

Body Fluid Compartments

  • Extracellular fluid (ECF) surrounds the cells.
  • Intracellular fluid (ICF) is inside cells.
  • Interstitial fluid (ISF) is a type of ECF.
  • Plasma is the fluid of blood.

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