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Questions and Answers
What is the main advantage of confocal microscopy over bright-field microscopy?
What is the main advantage of confocal microscopy over bright-field microscopy?
- Ability to work without a computer-driven mirror system
- Better resolution of the object in focus (correct)
- Higher magnification capabilities
- Improved contrast of the specimen components
What is the resolution range of the transmission electron microscope (TEM)?
What is the resolution range of the transmission electron microscope (TEM)?
- 30-40 nm
- Approximately 3 nm (correct)
- 200-300 nm
- Around 1 mm
How does a confocal microscope create an 'optical section' of a specimen?
How does a confocal microscope create an 'optical section' of a specimen?
- By capturing digital images at various depths in the specimen
- By capturing digital images at many spots in a very thin plane of focus (correct)
- By illuminating the entire specimen at once
- By using a series of filters to enhance contrast
What is the main function of the electromagnetic 'lenses' in a transmission electron microscope?
What is the main function of the electromagnetic 'lenses' in a transmission electron microscope?
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What role do cryofracture and freeze etching play in electron microscopy?
What role do cryofracture and freeze etching play in electron microscopy?
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