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Transmission Electron Microscopy

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Questions and Answers

What is the main advantage of confocal microscopy over bright-field microscopy?

Ability to work without a computer-driven mirror system

Better resolution of the object in focus (correct)

Higher magnification capabilities

Improved contrast of the specimen components

What is the resolution range of the transmission electron microscope (TEM)?

30-40 nm

Approximately 3 nm (correct)

200-300 nm

Around 1 mm

How does a confocal microscope create an 'optical section' of a specimen?

By capturing digital images at various depths in the specimen

By capturing digital images at many spots in a very thin plane of focus (correct)

By illuminating the entire specimen at once

By using a series of filters to enhance contrast

What is the main function of the electromagnetic 'lenses' in a transmission electron microscope?

<p>To focus the electron beam onto the specimen</p> Signup and view all the answers

What role do cryofracture and freeze etching play in electron microscopy?

<p>They aid in imaging membrane structures by breaking them along hydrophobic regions</p> Signup and view all the answers

Transmission Electron Microscopy

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Podcast

Questions and Answers

What is the main advantage of confocal microscopy over bright-field microscopy?

What is the resolution range of the transmission electron microscope (TEM)?

How does a confocal microscope create an 'optical section' of a specimen?

What is the main function of the electromagnetic 'lenses' in a transmission electron microscope?

What role do cryofracture and freeze etching play in electron microscopy?

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