Transmission Electron Microscopy

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Questions and Answers

What is the main advantage of confocal microscopy over bright-field microscopy?

  • Ability to work without a computer-driven mirror system
  • Better resolution of the object in focus (correct)
  • Higher magnification capabilities
  • Improved contrast of the specimen components

What is the resolution range of the transmission electron microscope (TEM)?

  • 30-40 nm
  • Approximately 3 nm (correct)
  • 200-300 nm
  • Around 1 mm

How does a confocal microscope create an 'optical section' of a specimen?

  • By capturing digital images at various depths in the specimen
  • By capturing digital images at many spots in a very thin plane of focus (correct)
  • By illuminating the entire specimen at once
  • By using a series of filters to enhance contrast

What is the main function of the electromagnetic 'lenses' in a transmission electron microscope?

<p>To focus the electron beam onto the specimen (A)</p> Signup and view all the answers

What role do cryofracture and freeze etching play in electron microscopy?

<p>They aid in imaging membrane structures by breaking them along hydrophobic regions (D)</p> Signup and view all the answers

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