5 Questions
What is the main advantage of confocal microscopy over bright-field microscopy?
Better resolution of the object in focus
What is the resolution range of the transmission electron microscope (TEM)?
Approximately 3 nm
How does a confocal microscope create an 'optical section' of a specimen?
By capturing digital images at many spots in a very thin plane of focus
What is the main function of the electromagnetic 'lenses' in a transmission electron microscope?
To focus the electron beam onto the specimen
What role do cryofracture and freeze etching play in electron microscopy?
They aid in imaging membrane structures by breaking them along hydrophobic regions
Learn about the principles and techniques of Transmission Electron Microscopy (TEM), including the use of heavy metal ions for contrast and resolution enhancement in tissue preparation. Explore the different regions of an electron micrograph and how they correspond to tissue areas with varying electron interactions.
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