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Questions and Answers
What is required for eukaryotic transcription initiation in vivo?
What is required for eukaryotic transcription initiation in vivo?
- Chromatin-modifying enzymes
- Mediator protein complex (correct)
- RNA polymerase II
- Transcriptional activators
Which proteins are involved in facilitating the assembly of the transcription initiation machinery onto DNA in eukaryotic cells?
Which proteins are involved in facilitating the assembly of the transcription initiation machinery onto DNA in eukaryotic cells?
- Histone-modifying enzymes
- Mediator protein complex
- RNA polymerase II
- Chromatin remodeling complexes (correct)
How many individual subunits approximately need to assemble at the start point of transcription to initiate transcription in a eukaryotic cell?
How many individual subunits approximately need to assemble at the start point of transcription to initiate transcription in a eukaryotic cell?
- Well over 100 subunits (correct)
- Around 50 subunits
- Exactly 100 subunits
- Less than 100 subunits
What is necessary for RNA polymerase II to begin transcribing?
What is necessary for RNA polymerase II to begin transcribing?
What characterizes the movement of RNA polymerase after transcription initiation?
What characterizes the movement of RNA polymerase after transcription initiation?
What happens after the process of abortive initiation is overcome?
What happens after the process of abortive initiation is overcome?
What happens when the polymerase encounters a terminator signal?
What happens when the polymerase encounters a terminator signal?
What is the role of the σ factor in transcription termination?
What is the role of the σ factor in transcription termination?
How does the transcription bubble change as the polymerase moves down the DNA?
How does the transcription bubble change as the polymerase moves down the DNA?
What characterizes a termination signal in most bacterial genes?
What characterizes a termination signal in most bacterial genes?
What is the role of TFIIH in the transcription initiation complex formation?
What is the role of TFIIH in the transcription initiation complex formation?
What is the significance of the C-terminal domain (CTD) of RNA polymerase during transcription initiation?
What is the significance of the C-terminal domain (CTD) of RNA polymerase during transcription initiation?
Which subunit of TFIIH enables RNA polymerase II to move away from the promoter and enter elongation?
Which subunit of TFIIH enables RNA polymerase II to move away from the promoter and enter elongation?
What is the function of RNA polymerase II at the promoter before entering the elongation phase?
What is the function of RNA polymerase II at the promoter before entering the elongation phase?
How does RNA polymerase II gain access to the template strand at the transcription start point?
How does RNA polymerase II gain access to the template strand at the transcription start point?
Which DNA sequence is considered the most important for most polymerase II promoters for transcription initiation?
Which DNA sequence is considered the most important for most polymerase II promoters for transcription initiation?
What is the function of TFIID in transcription initiation?
What is the function of TFIID in transcription initiation?
Which statement accurately describes the role of TFIIB in transcription initiation?
Which statement accurately describes the role of TFIIB in transcription initiation?
Where are most of the DNA sequences that influence transcription initiation located in relation to the transcription start point?
Where are most of the DNA sequences that influence transcription initiation located in relation to the transcription start point?
What role does the DPE sequence play in transcription initiation?
What role does the DPE sequence play in transcription initiation?
What is the function of the sigma (σ) factor in the RNA polymerase holoenzyme complex?
What is the function of the sigma (σ) factor in the RNA polymerase holoenzyme complex?
What is the role of the transcription bubble during RNA synthesis?
What is the role of the transcription bubble during RNA synthesis?
What happens when the polymerase holoenzyme slides into a promoter sequence?
What happens when the polymerase holoenzyme slides into a promoter sequence?
Why does the RNA polymerase holoenzyme dissociate when it collides with bacterial DNA?
Why does the RNA polymerase holoenzyme dissociate when it collides with bacterial DNA?
What is the main reason why several attempts to start polymerization often do not succeed?
What is the main reason why several attempts to start polymerization often do not succeed?
What happens to most of the general transcription factors once RNA polymerase II begins elongating the RNA transcript?
What happens to most of the general transcription factors once RNA polymerase II begins elongating the RNA transcript?
What additional function does the phosphorylation of the tail of RNA polymerase II serve?
What additional function does the phosphorylation of the tail of RNA polymerase II serve?
How does DNA packaging in eukaryotic cells affect transcription initiation complexity?
How does DNA packaging in eukaryotic cells affect transcription initiation complexity?
What role do activator, mediator, and chromatin-modifying proteins play in eukaryotic transcription initiation?
What role do activator, mediator, and chromatin-modifying proteins play in eukaryotic transcription initiation?
How do processing proteins interact with the nascent RNA molecule during transcription?
How do processing proteins interact with the nascent RNA molecule during transcription?
What is the main difference in the type of nucleotides linked by RNA polymerase compared to DNA polymerase?
What is the main difference in the type of nucleotides linked by RNA polymerase compared to DNA polymerase?
What unique capability does RNA polymerase have in initiating RNA chains compared to DNA polymerases?
What unique capability does RNA polymerase have in initiating RNA chains compared to DNA polymerases?
How does the processivity of RNA polymerase differ from that of DNA polymerases?
How does the processivity of RNA polymerase differ from that of DNA polymerases?
What characterizes the initiation of transcription in gene expression?
What characterizes the initiation of transcription in gene expression?
How does the recognition of start and stop points by RNA polymerase differ between bacteria and eukaryotes?
How does the recognition of start and stop points by RNA polymerase differ between bacteria and eukaryotes?
What is a significant feature of RNA polymerases regarding dissociation from the DNA template during transcription?
What is a significant feature of RNA polymerases regarding dissociation from the DNA template during transcription?
What is one key role of initiation in transcription in regulating gene expression?
What is one key role of initiation in transcription in regulating gene expression?
How does the ability to start an RNA chain without a primer benefit RNA polymerase?
How does the ability to start an RNA chain without a primer benefit RNA polymerase?
'Processivity' in terms of RNA polymerases refers to:
'Processivity' in terms of RNA polymerases refers to:
'Signals Encoded in DNA Tell RNA Polymerase Where to Start and Stop' - This statement highlights:
'Signals Encoded in DNA Tell RNA Polymerase Where to Start and Stop' - This statement highlights:
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