Molecular DIagnostics Lecture 2

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Questions and Answers

Which polymerase in eukaryotic transcription synthesizes 45s rRNA in the nucleolus?

  • Polymerase I (correct)
  • Mitochondrial polymerase
  • Polymerase II
  • Polymerase III

What is the purpose of the 5' cap in mRNA processing?

  • To guide mRNA translocation to the cytoplasm
  • To prevent elongation
  • To stabilize mRNA (correct)
  • To determine protein variation

Which region of DNA in eukaryotes affects the regulation of gene expression?

  • Promotor regions
  • Suppressor regions
  • Enhancer regions (correct)
  • Structural genes

Which type of RNA accounts for approximately 80% of the total RNA in rapidly growing mammalian cells?

<p>rRNA (B)</p> Signup and view all the answers

Which enzyme is involved in the removal of introns during mRNA processing?

<p>Spliceosome complex (D)</p> Signup and view all the answers

Which type of RNA polymerase synthesizes mRNA in the nucleoplasm?

<p>Polymerase II (D)</p> Signup and view all the answers

Which type of polymerase synthesizes mitochondrial RNA?

<p>Mitochondrial polymerase (D)</p> Signup and view all the answers

Which type of RNA accounts for approximately 15% of the total RNA in rapidly growing mammalian cells?

<p>tRNA (D)</p> Signup and view all the answers

Which type of RNA accounts for approximately 5% of the total RNA in rapidly growing mammalian cells?

<p>mRNA (B)</p> Signup and view all the answers

Which sugar is found in RNA?

<p>Ribose (B)</p> Signup and view all the answers

What is the base that is found in RNA but not in DNA?

<p>Uracil (U) (D)</p> Signup and view all the answers

What is the primary function of RNA polymerase?

<p>To catalyze RNA synthesis (B)</p> Signup and view all the answers

Which component of RNA polymerase recognizes the promoter region of DNA?

<p>Sigma (s) subunit (B)</p> Signup and view all the answers

What is the structure of RNA generally like?

<p>Single-stranded (A)</p> Signup and view all the answers

Which type of RNA makes up the majority of cellular RNA?

<p>Ribosomal RNA (rRNA) (A)</p> Signup and view all the answers

Which subunit of RNA polymerase binds to the promoter region of DNA?

<p>Omega (w) subunit (D)</p> Signup and view all the answers

What is the product of RNA synthesis?

<p>RNA molecule (D)</p> Signup and view all the answers

What type of bonds link the nucleotides in RNA?

<p>3’-5’ phosphodiester bonds (C)</p> Signup and view all the answers

What can RNA molecules do in terms of their 3D structure?

<p>Fold upon themselves to form helical regions (A)</p> Signup and view all the answers

What RNA structure is considered to be a part of the tertiary structures?

<p>pseudoknot (A)</p> Signup and view all the answers

elongation starts with a pyrimidine or purine?

<p>Purine</p> Signup and view all the answers

Inhibitors of RNA synthesis

<p>inhibits Template binding = Actinomycin inhibits polymerase binding = rifampin inhibits eukaryotic pol II and III = alpah-amanitin inhibits reverse transcription = AZT/ DDI</p> Signup and view all the answers

mRNA processing

<h1>Spliceosome = removal of introns/ connect exons 3' poly tail = adenosines added from mRNA 5, cap = 7-methylguanosine to 5' end</h1> Signup and view all the answers

Match the following inhibitors with the appropriate RNA synthesis process they inhibit:

<p>Actinomycin D = Template binding Rifampin = Polymerase binding a Amanitin = Inhibitors of euk. pol II and III AZT (azidothymidine) = Inhibitors of reverse transcription</p> Signup and view all the answers

Match the following types of RNA with the appropriate percentage they represent of the total RNA in rapidly growing mammalian cells:

<p>rRNA = ~ 80 percent tRNA = ~15 percent protein-coding mRNA = ~ 5 percent</p> Signup and view all the answers

Match the following eukaryotic polymerases with their function:

<p>Polymerase I = Synthesizes 45s rRNA in the nucleolus Polymerase II = Synthesizes mRNA, and others recognizes promotor sequence in the nucleoplasm Polymerase III = Synthesizes tRNA, 5sRNA, miRNA in the nucleus Mitochondrial polymerase = Synthesizes mitochondrial RNA</p> Signup and view all the answers

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