Transcription: DNA to mRNA

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Questions and Answers

What is the primary function of RNA polymerase in transcription?

  • To synthesize proteins from mRNA.
  • To replicate the entire DNA strand.
  • To add a protective cap to the DNA strand.
  • To catalyze the synthesis of mRNA from a DNA template. (correct)

Which event signals the beginning of transcription?

  • The detachment of RNA polymerase from the DNA.
  • The binding of mRNA to a ribosome.
  • The binding of RNA polymerase to a promoter region on the DNA. (correct)
  • The pairing of tRNA with mRNA.

Why is the addition of a 5' cap and a poly-A tail important for mRNA?

  • To facilitate the transcription process within the nucleus.
  • To protect the mRNA from degradation and enhance translation efficiency. (correct)
  • To prevent the mRNA from binding to ribosomes.
  • To ensure the mRNA is degraded immediately after synthesis.

If a DNA sequence reads 5'-GATTACA-3', what would be the corresponding mRNA sequence produced during transcription?

<p>5'-GUAAUAG-3' (C)</p>
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What potential consequence can arise from errors during the transcription process?

<p>Mutations affecting protein synthesis. (D)</p>
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Where does the mRNA molecule go after it has been processed?

<p>It moves to the ribosome for translation. (C)</p>
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Which of the following is NOT directly involved in the transcription process?

<p>Ribosomes (B)</p>
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What is the role of the promoter region in transcription?

<p>It serves as the binding site for RNA polymerase to initiate transcription. (A)</p>
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How does mRNA differ structurally from DNA?

<p>mRNA contains uracil, while DNA contains thymine. (B)</p>
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What is the immediate product of transcription?

<p>A primary transcript of mRNA (B)</p>
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Which of the following sequences represents a typical eukaryotic promoter region?

<p>TATA box (A)</p>
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What event occurs during mRNA processing after transcription?

<p>Introns are removed and exons are spliced together. (D)</p>
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What is the ultimate destination of mRNA after it leaves the nucleus?

<p>The ribosome (B)</p>
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How does the genetic code carried by mRNA relate to protein synthesis?

<p>It specifies the sequence of amino acids in the protein. (C)</p>
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Which of the following enzymes is responsible for unwinding the DNA double helix during transcription?

<p>RNA polymerase (A)</p>
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Why is it essential for mRNA to be processed before it leaves the nucleus?

<p>To protect it from degradation and ensure efficient translation. (D)</p>
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What would occur if the promoter region of a gene is mutated?

<p>Transcription of the gene may not occur. (B)</p>
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Which base pairing rule is followed during the transcription of DNA to mRNA?

<p>Adenine pairs with uracil; guanine pairs with cytosine. (B)</p>
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How does the length of the poly-A tail affect mRNA?

<p>It influences mRNA stability and translation efficiency. (B)</p>
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What is the main difference in location between transcription and translation in eukaryotic cells?

<p>Transcription occurs in the nucleus, while translation occurs in the cytoplasm. (D)</p>
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Flashcards

Transcription

The process where a DNA segment is copied into mRNA.

RNA polymerase

Enzyme that binds to DNA and synthesizes mRNA.

Promoter

DNA region where RNA polymerase binds to initiate transcription.

5' cap and poly-A tail

mRNA processing steps before leaving the nucleus.

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mRNA

Carries genetic code from DNA to ribosome for translation.

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Transcription errors

Can occur during transcription and affect protein synthesis.

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Study Notes

  • Transcription is the first step in gene expression.
  • A specific segment of DNA is copied into mRNA by RNA polymerase.
  • RNA polymerase binds to a promoter region on the DNA, initiating the unzipping of the double helix.
  • Complementary RNA nucleotides pair with the exposed DNA bases, forming a single-stranded mRNA molecule.
  • The mRNA strand undergoes processing, including the addition of a 5' cap and a poly-A tail, before it exits the nucleus.
  • mRNA carries the genetic code from the DNA to the ribosome.
  • The genetic code carried to the ribosome is then translated into a protein.
  • Errors during transcription can lead to mutations, which may affect protein synthesis.

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