Questions and Answers
What is the purpose of using two antibodies in the sandwich ELISA format?
The purpose of using two antibodies in the sandwich ELISA format is to have one antibody coated on the surface of the multi-well plate to facilitate the immobilization of the antigen, while the other antibody is conjugated and facilitates the detection of the antigen.
What are the two antibodies used in the sandwich ELISA format called?
The two antibodies used in the sandwich ELISA format are called matched antibody pairs.
What is the most commonly used format in ELISA?
The most commonly used format in ELISA is the sandwich ELISA (or sandwich immunoassay) format.
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