Tissue Processing in Histology
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Questions and Answers

What is the basic aim of tissue processing?

  • To clear the dehydrating agent from the tissue
  • To provide sufficient rigidity to the tissue for sectioning (correct)
  • To remove water from the tissue
  • To facilitate the transition from dehydration to impregnation

    • What is the purpose of dehydration in tissue processing?

  • To remove water from the tissue (correct)
  • To infiltrate the tissue with wax
  • To clear the dehydrating agent from the tissue
  • To facilitate the transition from dehydration to impregnation

    • What is the role of the clearing substance in tissue processing?

  • To infiltrate the tissue with wax
  • To provide rigidity to the tissue
  • To remove water from the tissue
  • To clear the dehydrating agent and facilitate the transition from dehydration to impregnation (correct)

    • What happens to the water within the tissue during tissue processing?

    <p>It is removed and replaced with a supporting medium</p> Signup and view all the answers

    What is the purpose of infiltration and impregnation in tissue processing?

    <p>To provide adequate rigidity to the tissue for sectioning</p> Signup and view all the answers

    What is the consequence of poor tissue processing?

    <p>Significant effects on section cutting and staining</p> Signup and view all the answers

    What is the effect of increasing the viscosity of the medium during tissue processing?

    <p>It decreases the penetration of the fluid</p> Signup and view all the answers

    What happens to the tissue if it is kept in the dehydrating fluid for too long?

    <p>It becomes hard and brittle</p> Signup and view all the answers

    What is the purpose of using a layer of anhydrous CuSO4 in the final alcohol bath?

    <p>To prevent staining of the tissue</p> Signup and view all the answers

    What is the purpose of agitation during tissue processing?

    <p>To facilitate the contact of tissue with fresh solution</p> Signup and view all the answers

    What is the purpose of dehydration in tissue processing?

    <p>To remove free or unbound water molecules from the tissue</p> Signup and view all the answers

    What is the effect of heat on tissue processing?

    <p>It increases the penetration of the fluid</p> Signup and view all the answers

    What is the purpose of removing the clearing agent from the tissue?

    <p>To prepare the tissue for sectioning</p> Signup and view all the answers

    What is the ideal characteristic of an impregnating medium?

    <p>It is miscible with the clearing agent</p> Signup and view all the answers

    Why is paraffin wax a popular embedding medium?

    <p>It is non-toxic and inexpensive</p> Signup and view all the answers

    What is the purpose of infiltration and impregnation?

    <p>To provide support to the tissue for sectioning</p> Signup and view all the answers

    What factors influence the processing of tissues?

    <p>Both the size and type of tissue</p> Signup and view all the answers

    What is the duration of the clearing process?

    <p>1 hour</p> Signup and view all the answers

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