Tissue Culture & Hybridoma Technology Quiz

Most cultured cells survive best if they are cooled at -10◦C/min.

Cellular metabolism continues even when all water in the system is converted to ice.

Fast cooling rates cause cell injury due to osmotic changes.

The major difficulty for cells at low temperatures is the ice-to-water phase transition.

The best method for cryopreserving cultured cells does not involve using cryoprotectants.

Slow cooling encourages the extracellular migration of water.

DMSO prevents ice crystals from forming in cells during the freezing process.

Ice crystals are beneficial for cell preservation during cryopreservation.

Fast cooling rates in cryopreservation lead to dehydration of cells.

Optimal cooling rates aim to avoid the formation of intracellular ice.

DMSO can pass through biological membranes with significant injury evidence.

High ionic concentration is beneficial for cells during cryopreservation.

Cryopreservation is not necessary for stem cells and other viable tissues because simple cooling or freezing can preserve them effectively for a long time.

Increasing the cell concentration before freezing can lead to better viability upon thawing.

DMSO is preferred over glycerol as a cryoprotectant because it penetrates the cell worse.

The ideal concentration range for DMSO in freezing medium is between 5-20%.

Increasing the serum concentration in freezing medium can lead to increased oxidative stress.

Thawed cells should be reseeded without diluting out the cryoprotectant for better survival rates.

Most cultured cells survive best if they are cooled at -1 °C/min.

During the Plateau Phase, the temperature curve increases as the liquid transitions into a semi-solid state.

The Final Cooling Phase is when the frozen solution is warmed to a higher temperature.

Storage at -80 °C is sufficient for long-term storage of most cryopreserved cells.

For long-term storage, frozen cells should be kept in cryofreezers that maintain temperatures above -150 °C.

Frozen cells stored at -196 °C may still exhibit metabolism, apoptosis, differentiation, and growth.

Vitrification involves the solidification of a liquid into a crystal structure.

The principle of vitrification method is to encourage ice crystal formation.

Low concentration of CPAs will promote vitrification in cells.

High concentration of CPAs leads to decreased medium toxicity.

DMSO, glycerol, ethylene glycol, and sucrose are examples of suitable cryoprotectant agents.

Vitrification is particularly useful for cryopreservation of adult stem cells.