Podcast
Questions and Answers
Most cultured cells survive best if they are cooled at -10◦C/min.
Most cultured cells survive best if they are cooled at -10◦C/min.
False
Cellular metabolism continues even when all water in the system is converted to ice.
Cellular metabolism continues even when all water in the system is converted to ice.
False
Fast cooling rates cause cell injury due to osmotic changes.
Fast cooling rates cause cell injury due to osmotic changes.
False
The major difficulty for cells at low temperatures is the ice-to-water phase transition.
The major difficulty for cells at low temperatures is the ice-to-water phase transition.
Signup and view all the answers
The best method for cryopreserving cultured cells does not involve using cryoprotectants.
The best method for cryopreserving cultured cells does not involve using cryoprotectants.
Signup and view all the answers
Slow cooling encourages the extracellular migration of water.
Slow cooling encourages the extracellular migration of water.
Signup and view all the answers
DMSO prevents ice crystals from forming in cells during the freezing process.
DMSO prevents ice crystals from forming in cells during the freezing process.
Signup and view all the answers
Ice crystals are beneficial for cell preservation during cryopreservation.
Ice crystals are beneficial for cell preservation during cryopreservation.
Signup and view all the answers
Fast cooling rates in cryopreservation lead to dehydration of cells.
Fast cooling rates in cryopreservation lead to dehydration of cells.
Signup and view all the answers
Optimal cooling rates aim to avoid the formation of intracellular ice.
Optimal cooling rates aim to avoid the formation of intracellular ice.
Signup and view all the answers
DMSO can pass through biological membranes with significant injury evidence.
DMSO can pass through biological membranes with significant injury evidence.
Signup and view all the answers
High ionic concentration is beneficial for cells during cryopreservation.
High ionic concentration is beneficial for cells during cryopreservation.
Signup and view all the answers
Cryopreservation is not necessary for stem cells and other viable tissues because simple cooling or freezing can preserve them effectively for a long time.
Cryopreservation is not necessary for stem cells and other viable tissues because simple cooling or freezing can preserve them effectively for a long time.
Signup and view all the answers
Increasing the cell concentration before freezing can lead to better viability upon thawing.
Increasing the cell concentration before freezing can lead to better viability upon thawing.
Signup and view all the answers
DMSO is preferred over glycerol as a cryoprotectant because it penetrates the cell worse.
DMSO is preferred over glycerol as a cryoprotectant because it penetrates the cell worse.
Signup and view all the answers
The ideal concentration range for DMSO in freezing medium is between 5-20%.
The ideal concentration range for DMSO in freezing medium is between 5-20%.
Signup and view all the answers
Increasing the serum concentration in freezing medium can lead to increased oxidative stress.
Increasing the serum concentration in freezing medium can lead to increased oxidative stress.
Signup and view all the answers
Thawed cells should be reseeded without diluting out the cryoprotectant for better survival rates.
Thawed cells should be reseeded without diluting out the cryoprotectant for better survival rates.
Signup and view all the answers
Most cultured cells survive best if they are cooled at -1 °C/min.
Most cultured cells survive best if they are cooled at -1 °C/min.
Signup and view all the answers
During the Plateau Phase, the temperature curve increases as the liquid transitions into a semi-solid state.
During the Plateau Phase, the temperature curve increases as the liquid transitions into a semi-solid state.
Signup and view all the answers
The Final Cooling Phase is when the frozen solution is warmed to a higher temperature.
The Final Cooling Phase is when the frozen solution is warmed to a higher temperature.
Signup and view all the answers
Storage at -80 °C is sufficient for long-term storage of most cryopreserved cells.
Storage at -80 °C is sufficient for long-term storage of most cryopreserved cells.
Signup and view all the answers
For long-term storage, frozen cells should be kept in cryofreezers that maintain temperatures above -150 °C.
For long-term storage, frozen cells should be kept in cryofreezers that maintain temperatures above -150 °C.
Signup and view all the answers
Frozen cells stored at -196 °C may still exhibit metabolism, apoptosis, differentiation, and growth.
Frozen cells stored at -196 °C may still exhibit metabolism, apoptosis, differentiation, and growth.
Signup and view all the answers
Vitrification involves the solidification of a liquid into a crystal structure.
Vitrification involves the solidification of a liquid into a crystal structure.
Signup and view all the answers
The principle of vitrification method is to encourage ice crystal formation.
The principle of vitrification method is to encourage ice crystal formation.
Signup and view all the answers
Low concentration of CPAs will promote vitrification in cells.
Low concentration of CPAs will promote vitrification in cells.
Signup and view all the answers
High concentration of CPAs leads to decreased medium toxicity.
High concentration of CPAs leads to decreased medium toxicity.
Signup and view all the answers
DMSO, glycerol, ethylene glycol, and sucrose are examples of suitable cryoprotectant agents.
DMSO, glycerol, ethylene glycol, and sucrose are examples of suitable cryoprotectant agents.
Signup and view all the answers
Vitrification is particularly useful for cryopreservation of adult stem cells.
Vitrification is particularly useful for cryopreservation of adult stem cells.
Signup and view all the answers
