Recent Lessons

Show all results for ""

Feature Overview

Ace your exams with our all-in-one platform for creating and sharing quizzes and tests.

Quizzes

Create quizzes and tests automatically from your content using AI.

Flashcards

Automatically turn your notes into digital flashcards.

Share, Export & Embed

Share with classmates or export to Excel and your learning management system.

Stats & Reporting

Auto-grading quizzes and tests with detailed stats and reports.

Mobile Apps

The smarter way to study – wherever you are.

Pricing

Search...

6 Questions

0 Views

Throat Culture Procedure

Choose a study mode

Play Quiz

Study Flashcards

Spaced Repetition

Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Why is it important to roll the swab back and forth near one edge of the blood agar plate before streaking?

To ensure the bacteria are evenly distributed across the agar surface

To prevent the swab from touching the agar surface, which could contaminate the plate

To create a concentrated area of bacteria for easier isolation and identification (correct)

To ensure the swab is fully saturated with bacteria before streaking

When performing a catalase test on a colony from a blood agar plate, why is extreme care necessary?

The catalase test is very sensitive and can be easily disrupted by contaminants.

Erythrocytes from the blood agar contain catalase, which can lead to a false-positive result. (correct)

The catalase test is only reliable when performed on pure cultures of bacteria.

Blood agar can be contaminated with other bacteria, which could interfere with the test results.

Which of the following is NOT a reason to incubate the blood agar plate inverted?

To increase the oxygen concentration available for the bacteria to grow (correct)

To ensure that the agar surface remains moist and suitable for bacterial growth

To prevent condensation from dripping onto the agar surface and interfering with colony growth

To reduce the risk of contamination from airborne microorganisms

What is the purpose of using a sterile tongue depressor in this procedure?

To prevent the tongue from touching the swab and potentially contaminating the sample Signup and view all the answers

What is the purpose of characterizing the colonies and hemolytic types?

To identify the specific bacteria present in the throat culture Signup and view all the answers

Why is it important to select well-isolated colonies for further testing?

To increase the likelihood of isolating a single bacterial species for identification Signup and view all the answers

Study Notes

Throat Culture Procedure

Collect a sterile throat swab sample, focusing on the tonsillar area.
Use a sterile tongue depressor to manipulate the tongue and avoid interference.
Streak the swab on a blood agar plate, rolling and streaking on one area of the plate.
Label the plate with the site sampled and the medium type.
Incubate the plate upside down at 35°C for 24-72 hours.
Inspect the plate for bacterial growth (colonies).
Identify the types of colonies based on characteristics.
Choose isolated colonies that match common throat pathogens.
Stain selected colonies using a Gram stain and conduct a catalase test on the same colonies.
Exercise extreme caution when transferring colonies from the blood agar plate to avoid false-positive catalase results due to possible blood cell contamination.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Test your knowledge on the throat culture procedure with this quiz. Covering steps from sample collection to bacterial identification, this quiz ensures you understand the necessary precautions and techniques involved. Perfect for students in microbiology or medical laboratory sciences.