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Test Your Understanding of PCR

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Questions and Answers

Which factor is most important in determining the optimal PCR annealing temperature?

Length of the primer

3' end stability of the primer

Melting temperature (Tm) (correct)

Base composition of the primer

What is the usual (G+C) content for ordinary PCR reactions?

Around 60-70%

Around 30-40%

Around 20-30%

Around 50-60% (correct)

What is the role of the DNA polymerase in PCR?

It stabilizes the 3' end of the primer

It anneals the primers to the DNA template

It determines the optimal annealing temperature

It synthesizes the new DNA strand (correct)

What is the typical length of a primer used in PCR?

Around 25 base pairs long Signup and view all the answers

What is the 3' end stability of a primer?

The ability of the primer to resist degradation Signup and view all the answers

What is the function of Taq polymerase in the PCR process?

It copies the region of the DNA fragment to be amplified Signup and view all the answers

What is the purpose of a thermal cycler in the PCR process?

To heat and cool the reaction tubes to the exact temperature required for each step of the reaction Signup and view all the answers

What are the basic components required for PCR?

DNA template, two primers, Taq polymerase, dNTPs, and divalent cations Signup and view all the answers

What is the purpose of PCR in medical and biological research labs?

To amplify a small amount of DNA fragments for various tasks Signup and view all the answers

What is the role of primers in the PCR process?

To bind to the beginning and end of the DNA fragment to be amplified Signup and view all the answers