Questions and Answers
Which factor is most important in determining the optimal PCR annealing temperature?
Melting temperature (Tm)
What is the usual (G+C) content for ordinary PCR reactions?
Around 50-60%
What is the role of the DNA polymerase in PCR?
It synthesizes the new DNA strand
What is the typical length of a primer used in PCR?
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What is the 3' end stability of a primer?
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What is the function of Taq polymerase in the PCR process?
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What is the purpose of a thermal cycler in the PCR process?
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What are the basic components required for PCR?
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What is the purpose of PCR in medical and biological research labs?
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What is the role of primers in the PCR process?
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