Questions and Answers
What is the TATA box?
Which subunit of GTF TFIID binds to the TATA box?
TATA binding protein (TBP)
What is the function of TFIIB in the transcription process?
Enables the adjacent binding of TFIIB
What happens after TFIIH binds at the transcription start point?
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TFIIH phosphorylates the polypeptide tail of RNA polymerase II, starting the elongation phase of transcription.
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Which component recognizes and binds RNA polymerase II to the TATA box?
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What does the transcription initiation complex consist of?
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What is the role of the TATA binding protein (TBP)?
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Study Notes
TATA Box
- A crucial DNA sequence located in the promoter region involved in the initiation of transcription.
Recognition by GTF
- GTF TFIID identifies and binds to the TATA box via the TATA binding protein (TBP), which is a specific subunit of TFIID.
Role of TFIID
- The binding of TFIID to the TATA box facilitates the subsequent attachment of TFIIB, another general transcription factor important for forming the transcription initiation complex.
Assembly of Transcription Complex
- Following the binding of TFIIB, other GTFs, including TFIIH, and RNA polymerase II converge at the promoter to create a complete transcription initiation complex.
Function of TFIIH
- TFIIH plays a key role by unwinding the DNA double helix at the transcription start site, utilizing ATP hydrolysis energy to expose the template strand for transcription.
RNA Polymerase II Activation
- TFIIH also phosphorylates the C-terminal domain of RNA polymerase II, facilitating its release from the GTFs and transitioning it into the elongation phase of transcription.
TFIID Functionality
- TFIID's role extends beyond recognition; it also binds RNA polymerase II to the TATA box, thereby initiating the transcription process.
TBP's Role
- TATA binding protein (TBP), a component of TFIID, induces bending of the DNA, which may attract additional GTFs essential for transcription initiation.
Transcription Initiation Complex Formation
- The assembly of general transcription factors (GTFs) and RNA polymerase II at the promoter results in the formation of a transcription initiation complex, a vital step in gene expression.
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Description
Test your knowledge on the TATA box and its role in DNA transcription. This quiz covers key definitions and functions related to the TATA box, including its interaction with general transcription factors. Perfect for students studying molecular biology or genetics.
