CUESTIONARIO PCR. PARTE 1

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Questions and Answers

¿Ante qué sospecha se suele solicitar la PCR?

  • Infección vírica.
  • Infección linfocítica.
  • Infección bacteriana grave. (correct)
  • Enfermedades inmunológicas

Seleccione Verdadero o Falso según corresponda. En personas con trastornos respiratorios crónicos, concentraciones elevadas de PCR suelen asociarse a brotes de la enfermedad o bien a ineficacia del tratamiento.

False (B)

¿Qué sugiere un aumento progresivo de su concentración sugiere la existencia de una inflamación, aunque no permite conocer la localización ni la causa de esta?

  • Un valor elevado de PCR (correct)
  • Una concentración elevada de PCR
  • En personas con trastornos inflamatorios crónicos
  • Infección bacteriana grave

La proteína C reactiva es un reactante de fase aguda sensible que se sintetiza en el:

<p>Hígado (B)</p> Signup and view all the answers

¿Cuál es la enzima clave utilizada en la PCR para sintetizar nuevas cadenas de ADN?

<p>Taq polimerasa (D)</p> Signup and view all the answers

¿Cuál de los siguientes pasos NO es parte de un ciclo de PCR típico?

<p>Transcripción del ARN (B)</p> Signup and view all the answers

¿Cuál de las siguientes es una aplicación importante de la PCR en la inmunología?

<p>Diagnóstico de infecciones virales como el VIH o hepatitis (A)</p> Signup and view all the answers

¿Qué tipo de muestras es más comúnmente utilizado en la PCR para diagnosticar infecciones respiratorias?

<p>Muestras de saliva (C)</p> Signup and view all the answers

¿Cómo se utiliza la PCR en el diagnóstico de infecciones virales?

<p>Para detectar el material genético del virus y evaluar la carga viral (D)</p> Signup and view all the answers

¿En qué etapa de la PCR ocurre la síntesis o elongación de la nueva cadena de ADN?

<p>Extensión (A)</p> Signup and view all the answers

Flashcards

PCR use in suspicion of infection

PCR (Polymerase Chain Reaction) is used to detect the presence of a pathogen's genetic material, aiding in diagnosing bacterial infections.

PCR and chronic respiratory issues

High PCR levels in patients with chronic respiratory conditions often indicate flare-ups or treatment failure, not necessarily a new infection.

PCR elevation and inflammation

A rising PCR level suggests inflammation, but doesn't pinpoint the location or cause.

PCR synthesis location

The liver is the primary site of protein C reactive (PCR) synthesis.

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Key PCR enzyme

Taq polymerase is the crucial enzyme used to synthesize new DNA strands during PCR.

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PCR step NOT included

Transcription of RNA isn't part of a typical PCR cycle; the process focuses on copying DNA.

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PCR in viral diagnosis

PCR is used to detect viral genetic material for diagnosis in cases like HIV or hepatitis, evaluating the viral load.

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Common PCR sample type for respiratory infections

Saliva samples are commonly used in PCR to detect respiratory infections.

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PCR viral diagnosis method

PCR identifies viral DNA/RNA and quantifies the virus's presence, evaluating its load.

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PCR DNA synthesis stage

The 'extension' stage in PCR is where the new DNA strand is synthesized.

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PCR

A technique to amplify small DNA or RNA sequences

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PCR application

Polymerase Chain Reaction is used in diagnosis, research, and forensic science.

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False PCR result

A PCR test can lead to an incorrect result due to cross-contamination or operator error.

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PCR and bacterial infections

PCR is helpful in identifying bacterial pathogens, providing information needed for treatment.

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Significance of Taq Polymerase

Taq Polymerase is heat-stable, vital for PCR's repeated heating and cooling steps.

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